Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNA<sup>Phe</sup>as a putative primer tRNA

Ono, M.; Ohishi, Hideyuki

doi:10.1093/nar/11.20.7169

Cited by 47 publications

(46 citation statements)

References 39 publications

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“…Consistent with these findings, our data indicate that early RT is inhibited by MOV10, suggesting that MOV10 could have affected the incorporation of the primer tRNA. Phenylalanine tRNA (tRNAPhe) was identified as the primer tRNA for IAP in mouse and hamster cells (18,19). However, we found that MOV10 did not interfere with tRNAPhe incorporation.…”

Section: Discussionmentioning

confidence: 91%

“…Decreased RT by MOV10 could also result from decreased incorporation of IAP RNA, which serves as the template for RT, or tRNAPhe, the tRNA that primes IAP RT (18,19). To determine if levels of IAP RNA or tRNAPhe in VLPs were decreased by MOV10, VLPs were isolated from cells expressing pFL with or without exogenous MOV10.…”

Section: Mov10 Inhibits Iap Rtp In a Dose-dependent Mannermentioning

confidence: 99%

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Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles

Luo

Jäger

et al. 2012

J Virol

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Moloney leukemia virus type 10 protein (MOV10) is an RNA helicase that is induced by type I interferon. It inhibits HIV replication at several steps of its replicative cycle. Of interest, MOV10 is a component of mRNA processing (P) bodies, which inhibit retrotransposition (RTP) of intracisternal A particles (IAP). In this report, we studied the effects of MOV10 on IAP RTP and its dependence on P bodies. Indeed, MOV10 inhibited IAP RTP. It decreased significantly not only the products of reverse transcriptase but also its endogenous activity. MOV10 also associated with IAP RNA. Furthermore, although it was found in IAP virus-like particles, it did not affect their incorporation of IAP RNA, primer tRNAPhe (phenylalanine tRNA), or IAP Gag. Concerning P bodies, the exogenously expressed MOV10 had no effect on their size and number, and the inhibition of IAP RTP persisted despite the depletion of their RCK subunit. Thus, by interfering with reverse transcription, MOV10 inhibits IAP RTP, and this inhibition is independent of P bodies.

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Section: Discussionmentioning

confidence: 91%

Section: Mov10 Inhibits Iap Rtp In a Dose-dependent Mannermentioning

confidence: 99%

Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles

Luo

Jäger

et al. 2012

J Virol

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“…However, one circle, spcB12, did possess a short region of homology ( Figure 3d) to a 14 bp sequence located 42 bp from the 5'-end of the LTR of an intracisternal A particle (IAP) from Syrian hamster (30). The Syrian hamster sequence is also identical to a sequence located in the same position in the LTR of the Mus musculus IAP rc mos (30).…”

Section: Bll 1 a G A T C T G C C T G C C T C T G A C T C C Cmentioning

confidence: 99%

“…The Syrian hamster sequence is also identical to a sequence located in the same position in the LTR of the Mus musculus IAP rc mos (30). IAP genes are known to compose a major class of copia like elements in rodents.…”

Section: Bll 1 a G A T C T G C C T G C C T C T G A C T C C Cmentioning

confidence: 99%

Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells

Stanfield

Helinski

1986

Nucl Acids Res

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Seven cloned small circular DNA molecules from CHO cells were sequenced and examined for the presence of homologies to each other and to a number of other functional sequences present in transposable elements, retroviruses, mammalian repeat sequences, and introns. The sequences of the CHO cell circular DNA molecules did not reveal common structural features that could explain their presence in the circular DNA population. A gene bank was constructed for CHO chromosomal DNA and sequences homologous to two of the seven small circular DNA molecules were isolated and sequenced. The nucleotide sequences present at the junction of circular and chromosomal DNA suggest that a recombination process involving homologous pairing may have been involved in the generation of one, but not the other, of the two circular DNA molecules.

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“…cles (IAPs) (7)(8)(9)(10)(11) in mice, have not been shown to produce infectious virions and hence are usually called retrovirus-like elements.…”

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confidence: 99%

Identification of a retrovirus-like repetitive element in human DNA.

Mager¹,

Henthorn²

1984

Proc. Natl. Acad. Sci. U.S.A.

126

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We describe a 5-to 6-kilobase-pair repetitive family in human DNA. One member of this family is linked to the ,-globin gene cluster and is close to the 3' breakpoints of three different naturally occurring deletions involving this gene cluster. Sequence analysis indicates that this element includes terminal direct repeats of 415 base pairs that exhibit the features of long terminal repeats (LTRs) of retroviruses. A potential histidine tRNA primer binding site occurs just 3' to the 5' direct repeat. This retrovirus-like element interrupts a member of the Kpn I family of repeated DNA and is bracketed by a 5-base-pair directly repeated sequence. When attempts are made to clone the element in bacteriophage, homologous recombination between the LTR-like sequences is very frequently observed. Copy number estimates by two methods indicate that the element is repeated 800-1000 times in the human genome. We term this Homo sapiens family of retroviruslike elements having a histidine tRNA primer binding site the hsRTVL-H family.Endogenous retroviral sequences have been the subject of considerable research over many years (1, 2), and their possible functions have been the cause of much speculation. Retroviral proviruses have been found in the normal DNA of a variety of species, but those found in chickens and mice are by far the best characterized (1, 3). Some endogenous proviruses can produce virus but others, such as the multicopy VL30 elements (4-6) and intracisternal A-type particles (IAPs) (7-11) in mice, have not been shown to produce infectious virions and hence are usually called retrovirus-like elements.There have been many attempts to identify endogenous proviruses in human DNA (12). The approach used most recently to search for such sequences is that of DNA hybridization of known retroviral sequences to normal human DNA. Using this method, several laboratories have detected and cloned human retrovirus-like DNA (13-16). The best characterized of these elements are homologous to Moloney leukemia virus, contain long terminal repeats (LTRs), and are present in multiple copies, some of which are transcribed (17-19).We report here the identification of a different human multicopy endogenous retrovirus-like element. Unlike the sequences previously described, this element was not detected by nucleic acid hybridization to viral probes. MATERIALS AND METHODSSources. Human DNA was isolated from cultured normal human embryonic fibroblasts (563 from Robert DeMars). The Kpn I family probes, pCa4A-10 and pCa4A-12, represent two halves of a long Kpn I family member and were kindly provided by Maxine Singer.Phage Cloning. The Xba I library was constructed by isolating the 9-to 18-kbp fragments of a complete Xba I digest of human DNA by preparative agarose gel electrophoresis and ligating these fragments into Xba I arms of DNA from X phage Charon 35 (23). The ligated DNA was then packaged in vitro (24). The size-selected HindIII, EcoRI, and Sst I libraries mentioned in the text were constructed by digesting human DNA to c...

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Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNA^Pheas a putative primer tRNA

Cited by 47 publications

References 39 publications

Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles

Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles

Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells

Identification of a retrovirus-like repetitive element in human DNA.

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Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNAPheas a putative primer tRNA

Cited by 47 publications

References 39 publications

Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles

Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles

Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells

Identification of a retrovirus-like repetitive element in human DNA.

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Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNA^Pheas a putative primer tRNA