Long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples

Sánchez-Martínez, M.L.; Aguilar-Caballos, M.P.; Gómez‐Hens, A.

doi:10.1016/j.talanta.2008.11.014

Cited by 17 publications

(3 citation statements)

References 14 publications

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“…Another novel colorimetric aptasensor was also developed for the sensitive detection of TC in honey (Wang, Liu, et al, 2015). Sánchez-Martínezet al developed a simple and rapid long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples, the LOD was 0.3 μg/kg (Sánchez-Martínez, Aguilar-Caballos, & Gómez-Hens, 2009). Another new efficient sensing instrumental for the detection has been observed based on aptasensor.…”

Section: Other Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

136

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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Section: Other Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

136

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“…A lot of researchers had employed various assay methods for determining some aminoglycosides including gentamicin in different delivery systems. These include: microbiological [5], polarographic [6], chemiluminescence [7], capillary electrophoresis [8], immunoassay [9], thin layer chromatographic [10], high performance liquid chromatographic [11], spectrofluorimetric [12], and spectrophotometric [13,14] methods.…”

Section: Introductionmentioning

confidence: 99%

Oral Gentamicin Preparation Using Solidified Lipid Particulate Delivery System

Adedokun

Jackson

Uchegbu

et al. 2017

Int J Pharm Pharm Sci

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Objective: Despite the broad pharmacological activity of gentamicin against a number of bacteria, it's very inadequate oral bioavailability due to poor intestinal membrane permeability has limited its formulation into oral dosage delivery system. This work was thus aimed at formulation and evaluation of gentamicin-loaded microemulsions based on preparation of lipid matrix for sustained release delivery. Methods:Oral gentamicin suspensions were prepared by emulsification method using Tween 80 as a mobile surfactant in the lipid matrix dispersion. The resultant oral suspensions were evaluated for mean particle size and morphology using a photomicrograph, encapsulation efficiency/entrapment, EE (%), dispersibility, pH and absolute drug content. Release study as a function of inhibition zone diameter (IZD) and in vitro release study was also carried out. The in vitro release study was performed in both simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 7.2) respectively. The release data were analyzed mathematically according to zero order, first order and Higuchi equations. Results:The prepared suspensions were cream-white in colour, easily dispersed and well homogenized. Batch D, which had least amount of excipients incorporated into the lipid matrix showed clumped irregular-shaped and less free-flowing particles. The particle size was significantly influenced by lipid matrix combination ratio in the presence of a surfactant (p<0.05). The mean particle size diameters of the samples were 15.44 mm, 10.64 mm, 4.12 mm, and 2.70 mm for batches A, B, C and D respectively. The values of EE obtained varied between 47% and 59% with Batch B exhibiting the highest value. The Higuchi model gave the best release kinetics result followed by zero order kinetics. Conclusion:Oral gentamicin prepared exhibited antibacterial properties against Klebsiella spp., Escherchia coli, Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. The results suggest that a lipid matrix system could be useful as a sustained release oral delivery system of a poorly absorbable drug such as gentamicin.

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“…The chemical structures of the studied drugs are shown in Table I. A wide variety of analytical methods was reported for determination of these drugs in pure forms, pharmaceutical formulations and biological fluids. These methods include spectrophotometric (3)(4)(5)(6)(7)(8)(9)(10)(11), spectrofluorimetric (12)(13)(14)(15)(16)(17)(18), chemiluminescence (19)(20)(21)(22)(23), thin-layer chromatographic (24)(25)(26), capillary electrophoresis (27)(28)(29), high-performance liquid chromatographic (30)(31)(32)(33)(34), polarographic (35,36), near infrared (37) immunoassay (38,39) and microbiological methods (40)(41)(42). In comparison with the previously published spectrofluorimetric methods that determined these drugs in micrograms per milliliter or nanograms per milliliter levels, the proposed method determines the concerned drugs in picograms per milliliter level.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Spectrofluorimetric Method for Determination of Certain Aminoglycosides in Pharmaceutical Formulations and Human Plasma

et al. 2013

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A simple, reliable, highly sensitive and selective spectrofluorimetric method has been developed for determination of certain aminoglycosides namely amikacin sulfate, tobramycin, neomycin sulfate, gentamicin sulfate, kanamycin sulfate and streptomycin sulfate. The method is based on the formation of a charge transfer complexes between these drugs and safranin in buffer solution of pH 8. The formed complexes were quantitatively extracted with chloroform under the optimized experimental conditions. These complexes showed an excitation maxima at 519-524 nm and emission maxima at 545-570 nm. The calibration plots were constructed over the range of 4-60 pg mL(-1) for amikacin, 4-50 pg mL(-1) for gentamicin, neomycin and kanamycin, 4-40 pg mL(-1) for streptomycin and 5-50 pg mL(-1) for tobramycin. The proposed method was successfully applied to the analysis of the cited drugs in dosage forms. The proposed method was validated according to ICH and USP guidelines with respect to specificity, linearity, accuracy, precision and robustness. The high sensitivity of the proposed method allowed determination of amikacin and gentamicin in spiked and real human plasma.

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Long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples

Cited by 17 publications

References 14 publications

Current advances in immunoassays for the detection of antibiotics residues: a review

Current advances in immunoassays for the detection of antibiotics residues: a review

Oral Gentamicin Preparation Using Solidified Lipid Particulate Delivery System

Highly Sensitive Spectrofluorimetric Method for Determination of Certain Aminoglycosides in Pharmaceutical Formulations and Human Plasma

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