Longer survival of rat limb allograft: Combined immunosuppression of FK-506 and 15-deoxyspergualin

Muramatsu, Keiichi; Doi, Kazuteru; Akino, Tatsuaki; Shigetomi, Mitsunori; Kawai, Shinya

doi:10.3109/17453679708999031

Cited by 30 publications

(12 citation statements)

References 13 publications

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“…9 -11 Vascularized bone allografts under immunosuppression, on the other hand, showed superior biological and biomechanical behavior with higher rates of bone integration. 7,[12][13][14] In this study we report on our experience with a double hand transplantation with special emphasis on the time course of callus formation and bone healing compared with hand replantation.…”

mentioning

confidence: 99%

Bilateral hand transplantation: Bone healing under immunosuppression with tacrolimus, mycophenolate mofetil, and prednisolone

Gabl

Pechlaner

Lutz

et al. 2004

The Journal of Hand Surgery

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mentioning

confidence: 99%

Bilateral hand transplantation: Bone healing under immunosuppression with tacrolimus, mycophenolate mofetil, and prednisolone

Gabl

Pechlaner

Lutz

et al. 2004

The Journal of Hand Surgery

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“…The previously described rat hindlimb replantation model was used. 7,8,18 The donor right limb, including the bone, muscles, femoral vessels, sciatic nerve, and skin, was amputated at midfemoral level. The skin distal to the knee joint was preserved to monitor circulation and skin rejection.…”

Section: Surgical Proceduresmentioning

confidence: 99%

“…In contrast, the skin component was rejected at 62 days posttransplant on average. 7,8 Control of bone allograft rejection requires understanding the rate and extent of graft replacement by recipient cells. Once complete, graft tolerance is likely.…”

mentioning

confidence: 99%

Gradual graft‐cell repopulation with recipient cells following vasularized bone and limb allotransplantation

Muramatsu¹,

Kurokawa²,

Kuriyama³

et al. 2005

Microsurgery

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Little is known about the fate of graft cells following vascularized bone allografting. This study was conducted to define the process of graft-cell repopulation with recipient cells. Sixty-five vascularized tibial bone and 50 limb allotransplantations were performed in rat sex-mismatched pairs. FK 506 was used for immunosuppression. The ratio of donor and recipient cells in the graft was evaluated by semiquantitative polymerase chain reaction, using the Y-chromosome primers. Allografted bones had no rejection episodes. In the vascularized bone allograft model, donor-derived cells were gradually replaced by cells of recipient origin, such that by 24 weeks, they comprised only 10% of total cells. In the limb allograft model, male recipient cells were detected in female grafts not at 1 week but at 48 weeks posttransplantation. The ratio of recipient cells was more than 10% in the femur and tibia. Recipient-derived cells gradually migrated into the grafted bone cells with the passage of time.

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“…18 We administered 1 mg/kg per day FK506 by intramuscular injection to the recipient rats for 7 days from the day of the surgery. 19 …”

Section: Methodsmentioning

confidence: 99%

Plasma microRNAs Are Potential Biomarkers of Acute Rejection After Hindlimb Transplantation in Rats

Oda

Ikeguchi

Yurie

et al. 2016

Transplantation Direct

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BackgroundThe development of effective immunosuppressive regimens has resulted in many cases of successful hand transplantation. Visual skin inspection and histological evaluation are used to assess the rejection of hand transplants, but these methods are largely subjective. In this study, we aimed to determine the potential of microRNAs (miRNAs) as biomarkers for acute rejection in vascularized composite allotransplants.MethodsIn allograft group, 7 male Brown-Norway rats (RT1n) were used as donors and 13 male Lewis rats (RT1l) were used as recipients. In control group, 8 Lewis rats were used as donors and recipients. The hindlimbs of donor rats were transplanted orthotopically to recipient rats. Skin changes were noted daily. Skin biopsies were obtained from 5 recipients and evaluated histologically. Plasma samples were obtained from the other 8 recipients before transplant and 7, 10, and 14 days posttransplant and used to measure miRNA expression.ResultsSkin changes occurred at a mean of 11.0 days posttransplant. Rejection in most skin biopsies taken 7 and 10 days posttransplant was histologically classified as grade 0, whereas that in most biopsies taken 14 days posttransplant was classified as grade 3. We found that expression of miRNA-146a and miRNA-155 was significantly upregulated at 10 and 14 days posttransplant compared with that at 7 days posttransplant. In control group, there were no significant changes in plasma miRNAs expressions.ConclusionsThe upregulation of plasma miRNA-146a and miRNA-155 was detected before the histological evaluation methods could diagnose complete rejection in the rat hindlimb transplantation model. Plasma miRNA-146a and miRNA-155 may be potential biomarkers of acute rejection after vascularized composite allotransplantation.

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Longer survival of rat limb allograft: Combined immunosuppression of FK-506 and 15-deoxyspergualin

Cited by 30 publications

References 13 publications

Bilateral hand transplantation: Bone healing under immunosuppression with tacrolimus, mycophenolate mofetil, and prednisolone

Bilateral hand transplantation: Bone healing under immunosuppression with tacrolimus, mycophenolate mofetil, and prednisolone

Gradual graft‐cell repopulation with recipient cells following vasularized bone and limb allotransplantation

Plasma microRNAs Are Potential Biomarkers of Acute Rejection After Hindlimb Transplantation in Rats

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