Longevity Effect of Liuwei Dihuang in Both Caenorhabditis Elegans and Aged Mice

Abstract

Liuwei Dihuang (LWDH), a famous traditional Chinese medicine, is widely used in the clinical treatment of aging-related diseases in China. However, its pharmacological mechanisms are not clear. In the present study, we evaluated the lifespan extension effect of LWDH in C. elegans and mice and revealed its underlying mechanisms. The results showed that LWDH significantly extended the lifespan of C. elegans in a dose-dependent manner. LWDH also conferred protection to nematodes against oxidative stress and reduced their fat storage. Genetics analysis and microarray data showed that the longevity effect of LWDH was attributed to the regulation of the innate immune response, proteolysis, lipid metabolism, and the oxidation-reduction process and was dependent on daf-16. Among the six herbs in the formula, Radix Rehmanniae Preparata and Fructus Macrocarpii contributed most to the longevity effect of this medicine, while the other four components had a synergistic effect on the longevity effect of the prescription. The lack of any single herb reduced the efficacy of the complete formula. LWDH also extended the lifespan and reduced both the weight and oxidant stress status in aged mice. Taken together, these results suggested that LWDH might function in a multi-target manner to extend the lifespan in both C. elegans and aged mice, and the best effect was achieved with the complete formula.

Exploring the Pharmacological Mechanism of Liuwei Dihuang Decoction for Diabetic Retinopathy: A Systematic Biological Strategy-Based Research

“…LDD also reduces H 2 O 2 -induced phosphorylation of p38 MAPK and p44/42 MAPKs (Erk1/2) [ 72 ] (see https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609941/figure/F3/ ). In experiments in which LDD prolonged the lifespan of C. elegans and mice, microarray data showed that the longevity effect of LDD was attributed to the regulation of innate immune response, proteolysis, lipid metabolism, and oxidative stress [ 73 ]. Vascular remodeling plays an important role in atherosclerosis in the retina.…”

A robust and miniaturized screening platform to study natural products affecting metabolism and survival in Caenorhabditis elegans

“…For preparation of a synchronized worm population N2 worms were chunked three days, SS104 worms were chunked four days before synchronization. Synchronization was performed by bleaching technique 39,94,95 . Briefly, worms were washed of the plates with ddH 2 O and treated with bleaching solution for approximately 5-10 min.…”

“…Briefly, 5-18 age-synchronized L1 nematodes were transferred by pipetting to 96-well microtiter plates with 6 mg/mL air dried OP50 in 120 µl S-complete medium, where they grow for 24 h at 25 °C until all worms reach the L3 stage. 5-Fluorodeoxyuridine (FUdR; 0.12 mM final; Sigma-Aldrich, F0503) is added in order to sterilize the worms and to keep the population synchronized 38,95 . The following day (day 0), test samples dissolved in DMSO were added to the sterilized adult worm culture.…”

Antioxidant and Lifespan-Extending Effects of a Rejuvenating Thai Traditional Polyherbal Remedy (Phy-Blica-O) in Caenorhabditis elegans

“…51 Several studies have demonstrated that their lifespan extension abilities and protective effects against age-related diseases of tonifying polyherbal mixtures are partially related to their antioxidant effects. [14][15][16][17] This tonifying agent uses as a whole-body tonic 21 and significantly extends the lifespan of C. elegans under the normal growth condition. The extract slightly reduced the accumulation of lipofuscin and enhanced the level of SOD production of C. elegans.…”