Longidorus carniolensis sp. n. (Nematoda, Longidoridae) from vineyard soil in Slovenia

Abstract

A new needle nematode, Longidorus carniolensis sp. n., recovered from the soil around the roots of grapevine Vitis vinifera L. from Slovenia, is described and illustrated. Longidorus carniolensisis an amphimictic species, characterised by females with a moderately long (L=5.6–8.2 mm) and plump (a=51–72.4, ave. 66.3) body, assuming a spiral to C-shape when heat relaxed. Head region continuous, anteriorly almost flat, lip region 23–25 µm wide; guiding ring situated posteriorly (42–47 μm, 43–50 μm in males), odontostyle long (ave. 146.6 (136–157) μm); pharyngeal glands with normal location, their nuclei of approximately equal size; tail bluntly conoidal to almost hemispherical. Males abundant, spicules slender and long (122–145 μm), ventromedian supplements 13–17, irregularly spaced, preceded by an adanal pair. Four juvenile stages present, the first stage juvenile with bluntly conoidal tail. Codes for identifying the new species when using the key by Chen et al. (1997) are: A 56, B 4, C 4, D 1, E 4, F 35, G 1, H 1, I 2. The new species is morphologically the most similar to Longidorus poessneckensis Altherr, 1974, Longidorus macrosoma Hooper, 1961, Longidorus caespiticola Hooper, 1961, Longidorus helveticus Lamberti et al., 2001, Longidorus macroteromucronatus Altherr, 1974, Longidorus pius Barsi & Lamberti, 2001, Longidorus raskii Lamberti & Agostinelli, 1993, Longidorus kheirii Pedram et al. 2008, Longidorus silvae Roca, 1993, Longidorus iuglandis Roca et al., 1985, Longidorus vinearum Bravo & Roca, 1995 and Longidorus major Roca & d’Erico, 1987, but differs from these species either by the body and odontostyle length, position of guide ring, head region and tail shape or the shape of the first stage juvenile tail. Sequence data from the D2-D3 region of the 28S rDNA distinguishes this new species from other speciesof the genus Longidorus with known sequences. Relationships of Longidorus carniolensis sp. n. with other Longidorus species based on analysis of this DNA fragment and morphology are discussed.

Description of Longidorus cholevae sp. n. (Nematoda, Dorylaimida) from a riparian habitat in the Rila Mountains, Bulgaria

“…Longidorus carniolensis – by having a shorter odontostyle (106–129 vs 136–157 μm); males with shorter spicules (96–120 vs 122–145 μm); different tail shape in first stage juvenile (elongate conoid vs rounded, conoidal, c’=1.8–2.2 vs c’=1.2–1.5) (Širca et al 2011);…”

Calcaridorylaimus castaneae sp. n. (Nematoda, Dorylaimidae) from Bulgaria with an identification key to the species of the genus

“…Presence of the conspicuous excretory pore observed in Calcaridorylaimus castaneae is unusual for members of Dorylaimida, however, similar structure has been mentioned only for two Longidorus species, namely Longidorus macrosoma Hooper, 1961 and Longidorus carniolensis Širca et al, 2011 ( Aboul-Eid 1969 , Širca et al 2011 ) and for several Mesodorylaimus species origination from Antarctica – Mesodorylaimus chipevi Nedelchev & Peneva, 2000, Mesodorylaimus antarcticus Nedelchev & Peneva, 2000, Mesodorylaimus masleni Nedelchev & Peneva, 2000, Mesodorylaimus imperator Loof, 1975 ( Nedelchev and Peneva 2000 ).…”