Longissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition

Poleti, Mirele Daiana; Regitano, L. C. A.; Souza, Gustavo H.M.F.; César, A. S. M.; Simas, Rosineide C.; Silva-Vignato, Bárbara; Oliveira, Guilherme Bueno de; Andrade, Sónia Cristina da Silva; Cameron, Luiz Cláudio; Coutinho, Luiz Lehmann

doi:10.1016/j.jprot.2018.02.028

Cited by 62 publications

(54 citation statements)

References 93 publications

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“…Protein expression can be measured using mass spectrometry. This can reveal novel biomarkers, such as tenderness and hypertrophy biomarkers (Picard et al, 2015), and provide a more reliable quantification of biological pathways (Poleti et al, 2018), especially regarding ECM deposition and posttranslational modification (Chen et al, 2019), as compared to RNA measurements. Several reviews were published regarding proteomics and farm animals skeletal muscle research, discussing biomarkers and metabolic pathways, which are related to myogenesis, hypertrophy and meat traits, including flavor, tenderness, color, water holding capacity and pH (Picard et al, 2010(Picard et al, , 2011Bendixen et al, 2011;Veiseth-Kent et al, 2018).…”

Section: Gene Expressionmentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

199

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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Section: Gene Expressionmentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

199

134

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“…Studies on the quality of beef have, therefore, continued to increase. Among the many factors affecting the quality of beef, intramuscular fat (IMF) plays an important role, affecting the tenderness, juiciness, and flavor of beef, especially for the production of "snowflake beef" [1][2][3][4]. At the same time, the price of marbled beef is also very high, with significant economic value.…”

Section: Introductionmentioning

confidence: 99%

Neudesin Neurotrophic Factor Promotes Bovine Preadipocyte Differentiation and Inhibits Myoblast Myogenesis

Wang

et al. 2019

Animals

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Neudesin neurotrophic factor (NENF) is a secreted protein that is essential in multiple biological processes, including neural functions, adipogenesis, and tumorigenesis. In our previous study, NENF was significantly inhibited in the bovine adipocytes-myoblasts co-culture system. However, studies on NENF regulation of bovine muscle development and involvement in the cross-talk between adipose tissue and skeletal muscle have not been reported. Hence, the aim of this study was to clarify the functional roles of NENF in bovine preadipocytes and myoblasts. Real-time quantitative PCR (RT-qPCR) was performed to examine the spatial expression patterns of NENF in different tissues, and the results showed that NENF was highly expressed in the muscle of four-day-old and 24-month-old Qinchuan cattle. Compared with four-day-old Qinchuan cattle, the expression level of NENF was significantly up-regulated in 24-month-old bovine adipose tissue. To explore the roles of NENF in bovine myoblast and preadipocyte differentiation, small interfering RNA (siRNA) targeting the NENF gene were transfected into bovine preadipocytes and myoblasts to knock down the expression of the NENF gene. The results showed that the knockdown of NENF in differentiating adipocytes attenuated lipid accumulation, decreased the mRNA expression of adipogenic key marker genes PPARγ, CEBPα, CEBPβ, FASN, and SCD1, and decreased the protein expression of PPARγ, CEBPα, and FASN. The formation of myotubes was significantly accelerated, and the mRNA expression levels of myogenic marker genes MYOD1, MYF5, MYF6, MEF2A, MEF2C, and CKM, and the protein expression levels of MYOD1, MYF6, MEF2A, and CKM were up-regulated in myoblasts where NENF was knocked down. In short, the knockdown of NENF inhibited preadipocyte differentiation and promoted myoblast myogenesis.

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“…A number of studies have investigated the differences in proteomic profiles in muscle tissue of livestock species 23 particularly in relation to meat quality characteristics such as muscle differentiation and growth, carcass composition, fat deposition [26][27][28] , and pre slaughter stress 29 . In addition, a study from our own group by Keady et al (2013) has shown differential muscle proteome expression across bovine breeds varying in genetic merit for carcass weight 24 .…”

mentioning

confidence: 99%

Label-free quantitative proteomic analysis of M. longissimus dorsi from cattle during dietary restriction and subsequent compensatory growth

Mullins

Keogh

Kenny

et al. 2020

Sci Rep

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compensatory growth (cG) is a naturally occurring physiological process whereby an animal has the ability to undergo enhanced growth following a period of restricted feeding. this studies objective was to identify key proteins involved in the expression of cG. forty Holstein friesian bulls were equally assigned to one of four groups. R1 and R2 groups were subjected to restricted feed allowance for 125 days (Period 1). A1 and A2 animals had ad libitum access to feed in Period 1. Following Period 1, all animals from R1 and A1 were slaughtered. Remaining animals (R2 and A2) were slaughtered following ad libitum access to feed for successive 55 days (Period 2). M. longissimus dorsi samples were collected at slaughter from all animals. proteins were isolated from samples and subjected to label-free mass spectrometry proteomic quantification. Proteins which were differentially abundant during CG (n = 39) were involved in cellular binding processes, oxidative phosphorylation and mitochondrial function. there was also evidence for up regulation of three pathways involved in nucleotide biosynthesis. Genetic variants in or regulating genes pertaining to proteins identified in this study may hold potential for use as DNA based biomarkers for genomic selection of animals with a greater ability to undergo CG. Compensatory growth (CG) or catch up growth can be defined as a physiological process through which an animal's growth rate accelerates upon re-alimentation following a period of under nutrition, enabling an animal to reach its genetically predetermined growth potential 1. In beef production systems, feed accounts for up to 80% of the total direct costs incurred 2. Therefore, any method by which these costs can be reduced, without impacting on overall animal performance, would positively influence on farm profitability. The exploitation of the CG phenomenon is a common practice in beef production systems, but particularly in pastoral systems such as in Ireland and has proven useful in reducing over winter animal feed costs 3. This naturally occurring biological process is also utilised in worldwide beef production systems in 'stocker' or 'backgrounding' programs 4. Additionally during CG, animal's exhibit enhanced feed efficiency, making it a highly desired trait 5,6. Due to the usefulness of this trait a number of studies have investigated the physiological 5-10 , as well as the molecular control 11-18 of CG in cattle. Skeletal muscle has a great economic value accounting for up to 50% of the total body weight of an animal and is in the order of 25% of basal metabolic expenditure 19,20. This tissue also plays a major role in energy homeostasis. Therefore skeletal muscle (M.longissimus dorsi) was selected as the target tissue for the current study. Transcriptional profiles have shown that both dietary restriction and subsequent CG are associated with greater expression of genes and pathways involved in metabolism, cellular function, energy production and cellular reorganization 13-15. However, although transcriptomic dat...

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Longissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition

Cited by 62 publications

References 93 publications

Tissue Engineering for Clean Meat Production

Tissue Engineering for Clean Meat Production

Neudesin Neurotrophic Factor Promotes Bovine Preadipocyte Differentiation and Inhibits Myoblast Myogenesis

Label-free quantitative proteomic analysis of M. longissimus dorsi from cattle during dietary restriction and subsequent compensatory growth

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