Longitalea arenae gen. nov., sp. nov. and Longitalea luteola sp. nov., two new members of the family Chitinophagaceae isolated from desert soil

Li, Shuai; Dong, Lei; Han, Jia-Rui; Shi, Guo-Yuan; Lu, Cuihua; Xu, Lu; Lian, Wen-Hui; Alkhalifah, Dalal Hussien M.; Hozzein, Wael N.; Li, Wen‐Jun

doi:10.1007/s00203-022-03119-x

Cited by 7 publications

(8 citation statements)

References 42 publications

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“…Growth of strains on R2A medium at various temperatures (4, 10, 15, 20, 25, 30, 37, 45 and 50 °C), pH values (pH 4, 5, 6, 7, 8, 9, 10, 11 and 12), and NaCl concentrations (0, 0.5, 1, 1.5, 2, 2.5, 3, 5, 7, and 10 %, w/v) was examined after 1 week of incubation [37]. The pH values were adjusted according to the method described by Li et al [36]. H 2 S production, hydrolysis of starch, CM-cellulose, gelatin, casein and Tweens (20, 40, 60 and 80) were tested as previously described [38,39].…”

Section: Physiologymentioning

confidence: 99%

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Desertivirga arenae gen. nov., sp. nov. and Desertivirga brevis sp. nov., isolated from desert soil, and reclassification of Pedobacter xinjiangensis as Desertivirga xinjiangensis comb. nov. and Pedobacter mongoliensis as Paradesertivirga mongoliensis gen.nov., comb. nov.

Li,

Liu,

Huang

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two novel bacterial strains, designated as SYSU D00823T and SYSU D00873T, were isolated from sandy soil of the Gurbantunggut Desert in Xinjiang, north-west China. SYSU D00823T and SYSU D00873T shared 99.0 % 16S rRNA gene sequence identity, and were both most closely related to Pedobacter xinjiangensis 12157T with 96.1 % and 96.0 % similarities, respectively. Phylogenetic and phylogenomic analyses revealed that the two isolates and P. xinjiangensis 12157T formed a separate distinct cluster in a stable subclade with the nearby species Pedobacter mongoliensis 1-32T, as well as the genera Pararcticibacter and Arcticibacter. Furthermore, P. mongoliensis 1-32T formed a separate deep-branching lineage and did not form a cluster with members of the genus Pedobacter. The average nucleotide identity and digital DNA–DNA hybridization values between SYSU D00823T and SYSU D00873T and related species were well below the thresholds for species delineation (<81.0 % and <24.0 %, respectively). The genomes of SYSU D00823T and SYSU D00873T were 6.19 and 6.43 Mbp in size with 40.4 % and 40.5 % DNA G+C contents, respectively. The predominant fatty acids (>10 %) of SYSU D00823T and SYSU D00873T were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). Menaquinone-7 was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, glycosphingolipid, aminoglycolipid/glycolipid, aminophospholipid and three or four unidentified polar lipids. These data indicated that strains SYSU D00823T and SYSU D00873T should be assigned to two novel species of a new genus within the family Sphingobacteriaceae, for which the names Desertivirga arenae gen. nov., sp. nov. and Desertivirga brevis sp. nov. are proposed. The type strains are SYSU D00823T (=CGMCC 1.18630T=MCCC 1K04973T=KCTC 82278T) and SYSU D00873T (=CGMCC 1.18629T=MCCC 1K04974T=KCTC 82281T), respectively. Accordingly, the reclassification of P. xinjiangensis as Desertivirga xinjiangensis comb. nov., and P. mongoliensis as Paradesertivirga mongoliensis gen. nov., comb. nov. are also proposed.

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Section: Physiologymentioning

confidence: 99%

“…The pH values were adjusted according to the method described by Li et al . [36]. H 2 S production, hydrolysis of starch, CM-cellulose, gelatin, casein and Tweens (20, 40, 60 and 80) were tested as previously described [38, 39].…”

Section: Physiologymentioning

confidence: 99%

Desertivirga arenae gen. nov., sp. nov. and Desertivirga brevis sp. nov., isolated from desert soil, and reclassification of Pedobacter xinjiangensis as Desertivirga xinjiangensis comb. nov. and Pedobacter mongoliensis as Paradesertivirga mongoliensis gen.nov., comb. nov.

Li,

Liu,

Huang

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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“…Anaerobic growth was determined using R2A broth at 30 °C for 7 days under anaerobic conditions as described by Li et al . [35]. The temperature range for growth was determined on R2A medium at 4, 15, 20, 25, 30, 37, 45 and 50 °C.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Cell motility was tested by the development of turbidity in semisolid R2A medium supplemented with 0.5 % agar (w/v). Anaerobic growth was determined using R2A broth at 30 °C for 7 days under anaerobic conditions as described by Li et al [35]. The temperature range for growth was determined on R2A medium at 4, 15, 20, 25, 30, 37, 45 and 50 °C.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Rufibacter roseolus sp. nov. and Rufibacter aurantiacus sp. nov., isolated from desert soil

Li,

Dong,

Lian

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two novel bacterial strains, designated as SYSU D00344T and SYSU D00433T, were isolated from soil of Gurbantunggut Desert in Xinjiang, north-west PR China. Cells of both strains were Gram-stain-negative, aerobic, short-rod-shaped, catalase-positive and non-motile. Oxidase activities of SYSU D00344T and SYSU D00433T were negative and positive, respectively. Optimal growth occurred at 30 °C, with 0–0.5 % (w/v) NaCl and at pH 7.0. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that they represented members of the genus Rufibacter and were closely related to Rufibacter hautae NBS58-1T. The results of phylogenomic analysis indicated that the two strains formed two independent and robust branches distinct from all reference type strains. The analyses of average nucleotide identity (ANI), digital DNA–DNA hybridisation (dDDH) values and 16S rRNA gene similarities between the two strains and their relatives further demonstrated that SYSU D00344T and SYSU D00433T represented two different novel genospecies. The polar lipids consisted of phosphatidylethanolamine, one unidentified glycolipid, two unidentified aminophospholipids, and two or four unidentified lipids. MK-7 was the only respiratory quinone. The major fatty acids (>10 %) for both strains were identified as iso-C15 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), as well as summed feature 4 (anteiso-C17 : 1B and/or iso-C17 : 1I) for SYSU D00344T and C16 : 1ω5c for SYSU D00433T. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genotypic characteristics, we propose Rufibacter roseolus sp. nov. and Rufibacter aurantiacus sp. nov. as two novel species in the genus Rufibacter . The type strains are SYSU D00344T (=CGMCC 1.8625T=MCCC 1K04971T=KCTC 82274T) and SYSU D00433T (=CGMCC 1.8617T=MCCC 1K04982T=KCTC 82277T), respectively.

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“…At the time of writing, the family Chitinophagaceae consists of 49 validly and corrected published genera (https://lpsn.dsmz.de/family/chitinophagaceae), with Chitinophaga as the type genus. These genera are wildly distributed in various habitats such as soil [2][3][4], air [5], sediment [6], and some other niches [7][8][9]. Members of the family Chitinophagaceae are usually characterized as Gram-stain-negative, thin and rod-shaped, aerobic or facultatively anaerobic, non-motile or swarming motile [10].…”

Section: Introductionmentioning

confidence: 99%

Aridibaculum aurantiacum gen. nov., sp. nov., isolated from the Kumtag Desert soil

Dong

Shi

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel orange-coloured bacterium, designated strain SYSU D00508T, was isolated from a sandy soil sampled from the Kumtag Desert in China. Strain SYSU D00508T was aerobic, Gram-stain-negative, oxidase-positive, catalase-positive and non-motile. Growth occurred at 4–45°C (optimum 28–30°C), pH 6.0–9.0 (optimum pH 7.0–8.0) and with 0–2.5 % NaCl (w/v, optimum 0–1.0 %). The major polar lipids consisted of phosphatidylethanolamine (PE), unidentified aminolipids (AL1-3) and unidentified polar lipids (L1-5) were also detected. The major respiratory quinone was MK-7 and the major fatty acids (>10 %) were iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G. The genomic DNA G+C content was 42.6 %. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU D00508T belonged to the family Chitinophagaceae and showed 93.9 % ( Segetibacter koreensis DSM18137T), 92.9 % ( Segetibacter aerophilus NBRC 106135T), 93.0 % ( Terrimonas soli JCM 32095T) and 92.8 % ( Parasegetibacter terrae JCM 19942T) similarities. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain SYSU D00508T is proposed to represent a novel species of a new genus, named Aridibaculum aurantiacum gen. nov., sp. nov., within the family Chitinophagaceae . The type strain is SYSU D00508T (=KCTC 82286T=CGMCC 1.18648T=MCCC 1K05005T).

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Longitalea arenae gen. nov., sp. nov. and Longitalea luteola sp. nov., two new members of the family Chitinophagaceae isolated from desert soil

Cited by 7 publications

References 42 publications

Desertivirga arenae gen. nov., sp. nov. and Desertivirga brevis sp. nov., isolated from desert soil, and reclassification of Pedobacter xinjiangensis as Desertivirga xinjiangensis comb. nov. and Pedobacter mongoliensis as Paradesertivirga mongoliensis gen.nov., comb. nov.

Desertivirga arenae gen. nov., sp. nov. and Desertivirga brevis sp. nov., isolated from desert soil, and reclassification of Pedobacter xinjiangensis as Desertivirga xinjiangensis comb. nov. and Pedobacter mongoliensis as Paradesertivirga mongoliensis gen.nov., comb. nov.

Rufibacter roseolus sp. nov. and Rufibacter aurantiacus sp. nov., isolated from desert soil

Aridibaculum aurantiacum gen. nov., sp. nov., isolated from the Kumtag Desert soil

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