Longitudinal Analysis of Feline Leukemia Virus-Specific Cytotoxic T Lymphocytes: Correlation with Recovery from Infection

Flynn, J.N.; Dunham, Stephen; Watson, Vivien C; Jarrett, Oswald

doi:10.1128/jvi.76.5.2306-2315.2002

Cited by 58 publications

(60 citation statements)

References 41 publications

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“…The finding of previously covert viral DNA in some cats which ostensibly totally resisted FeLV infection was not restricted to FeLV-61E-A, as other investigators found a similar phenomenon in cats challenged with the FeLV-A/Glasgow-1 Flynn et al, 2002;Tandon et al, 2005;Cattori et al, 2006;Gomes-Keller et al, 2006a;Gomes-Keller et al, 2006b;. It remained unknown, however, whether the viral DNA we detected by our qPCR assay represented intact, replication-competent provirus or replication-defective viral DNA sequences.…”

Section: Introductionmentioning

confidence: 39%

“…Application of qPCR to detect FeLV DNA in conjunction with p27 capsid capture ELISA has resulted in diagnostic sensitivity of 100% Flynn et al, 2002;Tandon et al, 2005;Torres et al, 2005;Cattori et al, 2006;Gomes-Keller et al, 2006a;Gomes-Keller et al, 2006b;Hofmann-Lehmann et al, 2006). In addition we, and others, found animals in which viral DNA was detected in the absence of detectable antigenemia, demonstrating that qPCR sensitivity was greater than p27 ELISA and that viral DNA burden was an accurate predictor of FeLV infection outcome.…”

Section: Discussionmentioning

confidence: 83%

“…We, and other investigators, developed a quantitative real-time FeLV DNA PCR assay (qPCR) as means to both diagnose FeLV infection and seek further insight into the determinants of the virus-host relationship Flynn et al, 2002;Tandon et al, 2005;Torres et al, 2005). When we applied DNA qPCR, together with the antigen capture ELISA, in vaccinated and unvaccinated cats challenged with FeLV-61E-A, we identified a spectrum of host-virus relationships in FeLV-exposed cats which did not develop persistent antigenemia (Torres et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

Torres¹,

O’Halloran²,

Larson³

et al. 2008

Veterinary Immunology and Immunopathology

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We previously defined four categories of FeLV infection, designated as abortive, regressive, latent, and progressive. To determine if detectable viral DNA is transcriptionally active in the absence of antigenemia, we developed and validated a real-time viral RNA qPCR assay. This assay proved to be highly sensitive, specific, reproducible, and allowed reliable quantitation. We then applied this methodology, together with real-time DNA qPCR and p27 capsid antigen capture ELISA, to examine cats challenged with FeLV. We found that circulating viral RNA and DNA levels were highly correlated and the assays were almost in perfect agreement. This indicates that the vast majority of viral DNA is transcriptionally active, even in the absence of antigenemia. The real-time qPCR assays are more sensitive than the most commonly used FeLV diagnostic assay, the p27 capsid antigen capture ELISA. Application of qPCR assays may add greater depth in understanding of FeLV-host relationships.

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Section: Introductionmentioning

confidence: 39%

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

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Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

Torres¹,

O’Halloran²,

Larson³

et al. 2008

Veterinary Immunology and Immunopathology

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“…Se o animal não desenvolve imunidade adequada, o vírus é disseminado sistemicamente por linfócitos e monócitos. A antigenemia/viremia é transiente, pois é detectável por cerca de três a seis semanas, ocorrendo completa eliminação do vírus após esse período (LUTZ et al, 1980;FLYNN et al, 2000;FLYNN et al, 2002); infecção latente -O animal apresenta antigenemia/ viremia por mais de três semanas e ocorre infecção de células da medula óssea (ROJKO et al, 1979;HARTMANN, 2006). Dentro de meses, o animal desenvolve uma resposta imune capaz de eliminar a antigenemia/viremia, mas o DNA proviral está presente nas células precursoras da medula.…”

Section: Patogenia Do Felv Com O Emprego De Técnicas Convencionais Deunclassified

“…O teste detecta animais positivos a partir da quarta semana após infecção (JARRET et al, 1982;FLYNN et al, 2002) e é recomendado como teste de triagem (LEVY et al, 2008;LUTZ et al, 2009).…”

Section: Introductionunclassified

Vírus da leucemia felina: análise da classificação da infecção, das técnicas de diagnóstico e da eficácia da vacinação com o emprego de técnicas sensíveis de detecção viral

Figueiredo

Júnior

2011

Cienc. Rural

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Feline Leukaemia Virus

Jarrett

Neil

2012

Encyclopedia of Life Sciences

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Feline leukaemia virus (FeLV) is a gammaretrovirus that causes a variety of fatal diseases including leukaemia and lymphoma in its host, the domestic cat, and occasionally in large cat species. Many cats become persistently infected and develop disease after an incubation period of several years, during which virus can be transmitted to other cats. Disease is often associated with the generation of FeLV variants with increased pathogenic potential that can arise by mutation of envelope sequences, duplication of transcriptional enhancer elements or recombination with host proto‐oncogene sequences. FeLV can also produce variants by recombination with endogenous proviruses related to FeLV that are found in the domestic cat and its close relatives. The prevalence of FeLV infection has been reduced in many cat populations by isolation of infected carriers and more recently by vaccines that augment the natural ability of most cats to control infection. Key Concepts: The outcome of infection by FeLV depends on the infectious dose, age and immunocompetence of the host. Many cats achieve lifelong control, if not elimination of infection. Cats that become persistently viraemic are at risk of a variety of FeLV‐related diseases after a latent period of up to several years. The common infectious form, FeLV‐A can acquire altered entry receptor specificity by envelope gene mutation (to FeLV‐C) or recombination with endogenous FeLV‐related proviruses (to FeLV‐B). FeLV envelope variants may induce fatal diseases of rapid onset including nonregenerative anaemia (FeLV‐C) or wasting and immunodeficiency (FeLV‐T). The oncogenic potential of FeLV can evolve by duplication of transcriptional enhancer control elements in the long‐terminal repeat or by transduction of host proto‐oncogene sequences. Although the immune parameters of vaccine protection remain unclear, FeLV demonstrates the feasibility of effective immunisation against the gamma retroviruses. Commercial FeLV vaccines in recent or current use include inactivated FeLV, infected cell extracts, recombinant envelope protein and live recombinant poxvirus vaccines.

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Longitudinal Analysis of Feline Leukemia Virus-Specific Cytotoxic T Lymphocytes: Correlation with Recovery from Infection

Cited by 58 publications

References 41 publications

Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

Vírus da leucemia felina: análise da classificação da infecção, das técnicas de diagnóstico e da eficácia da vacinação com o emprego de técnicas sensíveis de detecção viral

Feline Leukaemia Virus

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