Longitudinal Analysis of Genotype Distribution of Influenza A Virus from 2003 to 2005

Mak, Annie; Rahmanian, Ronak; Lei, Victor; Lawrence, David; Krajden, Mel; Brunham, Robert C.; Skowronski, Danuta M.; Li, Yan; Booth, Tim F.; Goh, Swee Han; Petric, Martin

doi:10.1128/jcm.02601-05

Cited by 9 publications

(5 citation statements)

References 19 publications

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“…Two sets of primers were used, resulting in 2 amplicons that overlap by ϳ300 bases. For each isolate, the amplicons were sequenced in both the 3' and 5' directions [36]. Sequences were annotated and compared with recently circulating H3N2 viruses.…”

Section: Methodsmentioning

confidence: 99%

Component‐Specific Effectiveness of Trivalent Influenza Vaccine as Monitored through a Sentinel Surveillance Network in Canada, 2006–2007

Serres²,

et al. 2009

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Section: Methodsmentioning

confidence: 99%

Component‐Specific Effectiveness of Trivalent Influenza Vaccine as Monitored through a Sentinel Surveillance Network in Canada, 2006–2007

Serres²,

et al. 2009

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“…Full length amplification of the NA gene with the H275 and H275Y alleles from pandemic (H1N1) 2009 virus was performed using primers described previously (Mak et al, 2006). These amplified fragments were cloned into the plasmid vector pCRII-TOPO ® and transformed into One Shot ® TOP-10 chemically competent Escherichia coli cells (Invitrogen, Carlsbad, CA, USA).…”

Section: In Vitro Transcribed Rnamentioning

confidence: 99%

Development of a real-time RT-PCR assay for detection of resistance to oseltamivir in influenza A pandemic (H1N1) 2009 virus using single nucleotide polymorphism probes

Wong

Fonseca

et al. 2011

Journal of Virological Methods

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Resistance to oseltamivir in pandemic (H1N1) 2009 influenza A virus is linked to an amino acid change from histidine (H) to tyrosine (Y) at position 275 in the neuraminidase protein (NA). A real-time one step RT-PCR assay using single nucleotide polymorphism (SNP) probes was developed to detect this mutation in respiratory specimens. The limit of detection was 47.6 copies/reaction for wild-type H275 RNA and 52.9 copies/reaction for the mutant H275Y RNA. The assay did not cross-react with other respiratory pathogens. The clinical sensitivity and specificity of the assay was compared to the gold standard Sanger sequencing method using 25 sensitive, 15 resistant and 20 negative samples. The sensitivity and specificity was 88.0% and 100% respectively with the SOIV_Osel_SEN probe designed to detect the H275 allele and 100% for the SOIV_Osel_RES probe detecting the 275Y allele. The sensitivity of the assay using nine admixtures of sensitive and resistant alleles was 88.9% and 77.8% with the SOIV_Osel_SEN probe and SOIV_Osel_RES probe respectively. The presence of mixed sensitive and resistant alleles in patient samples and mixtures of in vitro RNA were detected reproducibly. This assay can be used for screening of original samples for oseltamivir resistance without the need for culture and phenotypic testing.

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“…This would greatly enhance the ability to monitor the antigenic drift and to detect rarer antigenic shifts of the virus virtually in real-time basis. Finally, the identification and monitoring of such novel genotypes may allow to develop models that would predict the activity of influenza in following seasons [Ansaldi et al, , 2005Besselaar et al, 2004;Mak et al, 2006;Redlberger et al, 2007].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of influenza viruses circulating in Northern Italy during two seasons (2005/2006 and 2006/2007) of low influenza activity

Pariani

Amendola

Zappa

et al. 2008

Journal of Medical Virology

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The influenza activity and circulation of influenza viruses in Lombardy (the most populous Italian region) were observed during two consecutive seasons (2005/2006 and 2006/2007) characterized by low influenza activity by the Italian Influenza Surveillance Network. The molecular characteristics of circulating viruses were analyzed to evaluate the introduction of new variants and emergence of vaccine-escape viruses. In both seasons, the epidemic in Lombardy was sustained almost exclusively by influenza A viruses, accounting for 80.5% and 93.6% of total detections, respectively, and the co-circulation of A/H3 viruses belonging to distinct phylogenetic groups was observed. The A/H1N1 viruses isolated during the 2005/2006 season were closely related to A/New Caledonia/20/99, while the hemagglutinin (HA) sequences of the A/H1N1 viruses from the 2006/2007 season exhibited a greater diversity. These viruses were A/Solomon Islands/3/2006-like and showed several variants. All B isolates were similar to B/Malaysia/2506/2004 belonging to the B/Victoria/2/87-lineage. Influenza B virus was the dominant virus in Europe in the 2005/2006 season and accounted for the 20% of total detections in Lombardy. Overall, the viruses studied presented heterogeneity in their HA sequences suggesting the circulation of a miscellaneous set of variants during the two seasons notwithstanding the medium-low activity of influenza. The importance of virological surveillance of influenza viruses is recognized widely and the molecular characterization of the viruses, especially in vaccinated subjects, is of particular importance to evaluate the introduction and circulation of new variants.

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Longitudinal Analysis of Genotype Distribution of Influenza A Virus from 2003 to 2005

Cited by 9 publications

References 19 publications

Component‐Specific Effectiveness of Trivalent Influenza Vaccine as Monitored through a Sentinel Surveillance Network in Canada, 2006–2007

Component‐Specific Effectiveness of Trivalent Influenza Vaccine as Monitored through a Sentinel Surveillance Network in Canada, 2006–2007

Development of a real-time RT-PCR assay for detection of resistance to oseltamivir in influenza A pandemic (H1N1) 2009 virus using single nucleotide polymorphism probes

Molecular characterization of influenza viruses circulating in Northern Italy during two seasons (2005/2006 and 2006/2007) of low influenza activity

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