Longitudinal assessment of oxytocin efficacy on bone and bone marrow fat masses in a rabbit osteoporosis model through 3.0-T magnetic resonance spectroscopy and micro-CT

Qiu, Yue; Yao, Jing; Wu, Xing; Zhou, Baowen; Shao, Hongda; Ting, Hua; Xiong, Zhi Hua; Tang, Guang Yu

doi:10.1007/s00198-014-2933-9

Cited by 16 publications

(17 citation statements)

References 42 publications

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“…This size may affect the result stability of various examinations. Third, a histopathologic quantitative analysis of marrow adipose tissue was not performed in our study because FF value was considered to be a reliable index to represent the marrow adipose tissue in the human and rabbit (7,9,18). Fourth, decalcification may affect the CD34 immunochemical staining results because this process may inhibit CD34 protein expression to some extent.…”

Section: Discussionmentioning

confidence: 99%

“…The peak amplitudes of the water (4.65 parts per million [ppm]) and lipid (1.30 ppm) signals were measured and applied to calculate the bone marrow fat fraction (FF) (Fig 2). FF is defined as percentage of lipid signal intensity with respect to the total signal intensity (water and lipid) by using the following equation (17,18):…”

Section: Quantitative Dce Mr Examinationmentioning

confidence: 99%

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Reduction of Longitudinal Vertebral Blood Perfusion and Its Likely Causes: A Quantitative Dynamic Contrast-enhanced MR Imaging Study of a Rat Osteoporosis Model

Zhu¹,

Zhang²,

Wu³

et al. 2017

Radiology

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Purpose To determine the longitudinal relationships among lumbar vertebral blood perfusion, bone mass, and marrow adipose tissue in a rat osteoporosis model after ovariectomy by using quantitative dynamic contrast agent-enhanced (DCE) magnetic resonance (MR) imaging, microcomputed tomography (micro-CT), and proton MR spectroscopy. Materials and Methods In this animal review committee-approved study, lumbar vertebrae were evaluated through MR spectroscopy, quantitative DCE MR imaging, micro-CT, and histopathologic analysis, and blood was examined at 0, 2, 6, 10, 14, 18, and 24 weeks after ovariectomy consisting of exposure of the ovaries but no excision (n = 35) or sham operation, defined as exposure of the ovaries but no excision (n = 35). Differences in the parameters of these examinations between two groups at the same time point were analyzed by an independent-sample t test. Results Significantly reduced volume transfer constant (K) and volume of extravascular extracellular space per unit volume of tissue (week 2 and 10, respectively; P = .036 and P = .014, respectively), decreased bone mineral density (week 2; P = .014), and increased fat fraction (week 6; P = .036) in the ovariectomy group were observed, compared with those in the sham group. Vascular endothelial growth factor and microvessel density values of the ovariectomy group decreased significantly compared with those of the sham group from weeks 18 (P = .005) and 14 (P = .018), respectively. Transmission electron microscopy revealed tighter gaps among vascular endothelial cells and more marrow fibrosis in the ovariectomy group. Conclusion Quantitative DCE MR imaging can directly reflect marrow perfusion. K is a promising parameter to demonstrate early reduced marrow perfusion. Enhanced vasoconstriction and tightened gaps among vascular endothelial cells may be the likely causes in the initial stage of osteoporosis. Increased marrow adipose tissue, decreased microvessel density, and increased marrow fibrosis may aggravate marrow ischemia in the late stage of osteoporosis. RSNA, 2016 Online supplemental material is available for this article.

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Section: Discussionmentioning

confidence: 99%

Section: Quantitative Dce Mr Examinationmentioning

confidence: 99%

Reduction of Longitudinal Vertebral Blood Perfusion and Its Likely Causes: A Quantitative Dynamic Contrast-enhanced MR Imaging Study of a Rat Osteoporosis Model

Zhu¹,

Zhang²,

Wu³

et al. 2017

Radiology

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“…The OVX-vehicle group was subjected to OVXonly. The OVX-oxytocin group was treated with OVX and subcutaneous injection of 1 mg/kg of oxytocin daily for 10 days after OVX or up to the experimental points [15, 18]. To prevent infection, rabbits all received antibiotics (penicillin 4 × 105 U·kg − 1, i.m.b.…”

Section: Methodsmentioning

confidence: 99%

Bone microarchitectural parameters can detect oxytocin induced changes prior to bone density on mitigating bone deterioration in rabbit osteoporosis model using micro-CT

Qiu

Tang

Serrano‐Sosa

et al. 2019

BMC Musculoskelet Disord

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BackgroundThis study is aimed to determine the efficacy of X-Ray Microtomography (micro-CT) in predicting oxytocin (OT) treatment response in rabbit osteoporosis(OP) model.MethodsSixty-five rabbits were randomly divided into three groups: control group, ovariectomy (OVX) -vehicle and OVX-oxytocin group. The controls underwent sham surgery. OVX-vehicle and OVX-oxytocin groups were subjected to bilateral OVX. The rabbits in OVX-oxytocin group were injected with oxytocin. In the 0th, 4th, 8th, 10th and 12th weeks post OVX operation, bone mineral density (BMD) and bone micro-architectural parameters were measured in three groups.ResultsBone mineral density (BMD), bone volume fraction (BV/TV), Trabecular Number (Tb.N), and Trabecular Thickness (Tb.Th) decreased, while Trabecular Spacing (Tb.Sp) and Structure Model Index (SMI) increased overtime in all the three groups. In OVX-oxytocin group, the bone deterioration tendency is slowing down compared with that of the OVX-vehicle group. The BMD of the OVX-oxytocin group was significantly lower than those in the OVX-vehicle group at 12th week (P = 0.017). BV/TV and Tb.Sp in OVX-oxytocin group changed significantly from 8th week (P = 0.043) and 12th week (P = 0.014), which is earlier than that of BMD and other bone micro-architectural parameters.ConclusionBV/TV and Tb.Sp changed prior to BMD and other bone micro-architectural parameters with oxytocin intervention, which indicate that they are more sensitive markers for predicting early osteoporosis and treatment monitoring when using micro-CT to evaluate osteoporosis rabbit model.

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“…One of the key findings in the present study was that early concurrent OT treatment resulted in full recovery of glucocorticoid-induced marrow adiposity but partial recovery of BMD. Interestingly, a recent publication with similar methods presented by Qiu et al (20) indicated that OT treatment from 2 weeks after ovariectomy resulted in partial recovery of marrow fat accumulation by decreasing marrow adipocyte size and density in ovariectomized rabbits. However, Castaneda et al (21) reported that osteoporosis can be induced experimentally in rabbits through isolated glucocorticoid or by a combination of ovariectomy and medium dose corticosteroid for 4 weeks.…”

Section: Discussionmentioning

confidence: 59%

MR spectroscopy for assessing the effects of oxytocin on marrow adipogenesis induced by glucocorticoid in rabbits

Jiang

Sun

et al. 2015

Acta Radiol

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Longitudinal assessment of oxytocin efficacy on bone and bone marrow fat masses in a rabbit osteoporosis model through 3.0-T magnetic resonance spectroscopy and micro-CT

Abstract: Early in vivo OT treatment slowed down bone deterioration and reduced bone marrow adiposity accumulation in a rabbit OP model, which is consistent with pathologic findings. OT treatment is a promising preventive OP therapy.

Cited by 16 publications

References 42 publications

Reduction of Longitudinal Vertebral Blood Perfusion and Its Likely Causes: A Quantitative Dynamic Contrast-enhanced MR Imaging Study of a Rat Osteoporosis Model

Reduction of Longitudinal Vertebral Blood Perfusion and Its Likely Causes: A Quantitative Dynamic Contrast-enhanced MR Imaging Study of a Rat Osteoporosis Model

Bone microarchitectural parameters can detect oxytocin induced changes prior to bone density on mitigating bone deterioration in rabbit osteoporosis model using micro-CT

MR spectroscopy for assessing the effects of oxytocin on marrow adipogenesis induced by glucocorticoid in rabbits

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