Longitudinal assessment of rabbit renal fibrosis induced by unilateral ureteral obstruction using two‐dimensional susceptibility weighted imaging

Zhang, Jinggang; Zhou, Xing; Zha, Tingting; Tian, Xiaojuan; Du, Yanan; Chen, Jie; Xing, Wei

doi:10.1002/jmri.25915

Cited by 15 publications

(12 citation statements)

References 33 publications

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“…The ability of SWI in noninvasive measurement of renal fibrosis has been shown in a rabbit model of UUO. 150,151 By incorporating sophisticated mathematical analysis, quantitative susceptibility mapping can be used to extract susceptibility maps from susceptibility-weighted images. Xie et al demonstrated a good sensitivity of high-resolution quantitative susceptibility mapping in detecting renal fibrosis in fixed kidneys of mice deficient for angiotensin receptor type-1.…”

Section: Other Mri Techniquesmentioning

confidence: 99%

Noninvasive assessment of renal fibrosis by magnetic resonance imaging and ultrasound techniques

Jiang

Ferguson

Lerman

2019

Translational Research

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Renal fibrosis is a useful biomarker for diagnosis and guidance of therapeutic interventions of chronic kidney disease (CKD), a worldwide disease that affects more than 10% of the population and is one of the major causes of death. Currently, tissue biopsy is the gold standard for assessment of renal fibrosis. However, it is invasive, and prone to sampling error and observer variability, and may also result in complications. Recent advances in diagnostic imaging techniques, including magnetic resonance imaging (MRI) and ultrasonography, have shown promise for noninvasive assessment of renal fibrosis. These imaging techniques measure renal fibrosis by evaluating its impacts on the functional, mechanical, and molecular properties of the kidney, such as water mobility by diffusion MRI, tissue hypoxia by blood oxygenation level dependent MRI, renal stiffness by MR and ultrasound elastography, and macromolecule content by magnetization transfer imaging. Other MR techniques, such as T 1 /T 2 mapping and susceptibility-weighted imaging have also been explored for measuring renal fibrosis. Promising findings have been reported in both preclinical and clinical studies using these techniques. Nevertheless, limited specificity, sensitivity, and practicality in these techniques may hinder their immediate application in clinical routine. In this review, we will introduce methodologies of these techniques, outline their applications in fibrosis imaging, and discuss their limitations and pitfalls.

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Section: Other Mri Techniquesmentioning

confidence: 99%

Noninvasive assessment of renal fibrosis by magnetic resonance imaging and ultrasound techniques

Jiang

Ferguson

Lerman

2019

Translational Research

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“…Obstructive nephropathy may lead to Acute Kidney Injury (AKI) or Chronic Kidney Disease (CKD) [3,4,5]. CKD involves the progressive decrease in renal function and the alteration of renal structure lasting more than three months [6]. CKD is characterized by the loss of epithelial renal cells and their replacement with extracellular matrix (ECM) in the glomeruli and tubulointerstitium [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…CKD involves the progressive decrease in renal function and the alteration of renal structure lasting more than three months [6]. CKD is characterized by the loss of epithelial renal cells and their replacement with extracellular matrix (ECM) in the glomeruli and tubulointerstitium [5,6]. Patients with CKD are usually diagnosed after ECM accumulation has initiated [7] and show evidence of the effect of Reactive Oxygen Species (ROS)-induced damage in proteins, lipids and DNA [8].…”

Section: Introductionmentioning

confidence: 99%

Unilateral Ureteral Obstruction as a Model to Investigate Fibrosis-Attenuating Treatments

et al. 2019

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Renal fibrosis is the common pathway for most forms of progressive renal disease. The Unilateral Ureteral Obstruction (UUO) model is used to cause renal fibrosis, where the primary feature of UUO is tubular injury as a result of obstructed urine flow. Furthermore, experimental UUO in rodents is believed to mimic human chronic obstructive nephropathy in an accelerated manner. Renal fibrosis is the common pathway for most forms of progressive renal disease. Removing the obstruction may not be sufficient to reverse fibrosis, so an accompanying treatment may be of benefit. In this review, we have done a revision on treatments shown to ameliorate fibrosis in the context of the UUO experimental model. The treatments inhibit the production of fibrotic and inflammatory proteins such as Transforming Growth Factor β1 (TGF-β1), Tumor Necrosis Factor α (TNF-α), collagen and fibronectin, Heat Shock Protein 47 (HSP47), suppress the proliferation of fibroblasts, prevent epithelial-to-mesenchymal transition, reduce oxidative stress, inhibit the action of the Nuclear Factor κB (NF-κB), reduce the phosphorylation of mothers against decapentaplegic homolog (SMAD) family members 2 and 3 (Smad2/3) or Mitogen-Activated Protein Kinases (MAPKs), inhibit the activation of the renin-angiotensin system. Summaries of the UUO experimental methods and alterations observed in the UUO experiments are included.

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“…After anesthesia, each rabbit was placed in the right lateral position. Consistent with previous literature, the operation was performed on the left kidney since the left kidney of rabbit is not hidden by the liver and ribs, making the surgery relatively easy. The left waist was incised by scalpel to 7 cm.…”

Section: Methodsmentioning

confidence: 99%

Acute renal impairment characterization using diffusion magnetic resonance imaging: Validation by histology

Zhu

et al. 2019

NMR in Biomedicine

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Diffusion magnetic resonance imaging has been demonstrated to be a simple, noninvasive and accurate method for the detection of renal microstructure and microcirculation, which are closely linked to renal function. Moreover, serum endothelin‐1 (ET‐1) was also reported as a good indicator of early renal injury. The aim of this study was to evaluate the feasibility and capability of diffusion MRI and ET‐1 to detect acute kidney injury by an operation simulating high‐pressure renal pelvic perfusion, which is commonly used during ureteroscopic lithotripsy. Histological findings were used as a reference. Fourteen New Zealand rabbits in an experimental group and 14 in a control group were used in this study. Diffusion tensor imaging and intravoxel incoherent motion diffusion‐weighted imaging were acquired by a 3.0 T MRI scanner. Significant corticomedullary differences were found in the values of the apparent diffusion coefficient (ADC), pure tissue diffusion, volume fraction of pseudo‐diffusion (fp) and fractional anisotropy (FA) (P < 0.05 for all) in both preoperation and postoperation experimental groups. Compared with the control group, the values of cortical fpmean, medullary ADCmean and FAmean decreased significantly (P < 0.05) after the operation in the experimental group. Also, the change rate of medullary ADCmean in the experimental group was more pronounced than that in the control group (P = 0.018). No significant change was found in serum ET‐1 concentration after surgery in either the experimental (P = 0.80) or control (P = 0.17) groups. In the experimental group, histological changes were observed in the medulla, while no visible change was found in the cortex. This study demonstrated the feasibility of diffusion MRI to detect the changes of renal microstructure and microcirculation in acute kidney injury, with the potential to evaluate renal function. Moreover, the sensitivity of diffusion MRI to acute kidney injury appears to be superior to that of serum ET‐1.

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Longitudinal assessment of rabbit renal fibrosis induced by unilateral ureteral obstruction using two‐dimensional susceptibility weighted imaging

Abstract: 2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2018;47:1572-1577.

Cited by 15 publications

References 33 publications

Noninvasive assessment of renal fibrosis by magnetic resonance imaging and ultrasound techniques

Noninvasive assessment of renal fibrosis by magnetic resonance imaging and ultrasound techniques

Unilateral Ureteral Obstruction as a Model to Investigate Fibrosis-Attenuating Treatments

Acute renal impairment characterization using diffusion magnetic resonance imaging: Validation by histology

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