Longitudinal axons are guided by Slit/Robo signals from the floor plate

Mastick, Grant S.; Wt, Farmer; Al, Altick; Hf, Nural; Jp, Dugan; Kidd, Thomas; Charron, Frédéric

doi:10.4161/cam.4.3.11219

Cited by 18 publications

(18 citation statements)

References 29 publications

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“…Slit-mediated repulsion specifies three lateral positions (medial, intermediate, and lateral) for distinct longitudinal axon tracts based on differential expression of Robo receptors (Evans and Bashaw, 2010; Rajagopalan et al, 2000; Simpson et al, 2000; Spitzweck et al, 2010). Related functions of Slit-Robo signaling for CNS longitudinal tract formation have also been observed in vertebrates (Farmer et al, 2008; Long et al, 2004; Lopez-Bendito et al, 2007; Mastick et al, 2010). Interestingly, sensory afferent input to the Drosophila embryonic CNS utilizes this same Slit-Robo code to regulate the projection of different sensory axon classes to distinct CNS lateral positions (Zlatic et al, 2003), restricting both the pre- and postsynaptic components of this first synapse for sensory circuits to a limited region.…”

Section: Introductionmentioning

confidence: 74%

A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS

Sweeney

Ayoob

et al. 2011

Neuron

145

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SUMMARY Longitudinal axon fascicles within the Drosophila embryonic CNS provide connections between body segments and are required for coordinated neural signaling along the anterior-posterior axis. We show here that establishment of select CNS longitudinal tracts and formation of precise mechanosensory afferent innervation to the same CNS region are coordinately regulated by the secreted semaphorins Sema-2a and Sema-2b. Both Sema-2a and Sema-2b utilize the same neuronal receptor, plexin B (PlexB), but serve distinct guidance functions. Localized Sema-2b attraction promotes the initial assembly of a subset of CNS longitudinal projections and subsequent targeting of chordotonal sensory afferent axons to these same longitudinal connectives, while broader Sema-2a repulsion serves to prevent aberrant innervation. In the absence of Sema-2b or PlexB, chordotonal afferent connectivity within the CNS is severely disrupted, resulting in specific larval behavioral deficits. These results reveal that distinct semaphorin-mediated guidance functions converge at PlexB and are critical for functional neural circuit assembly.

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Section: Introductionmentioning

confidence: 74%

A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS

Sweeney

Ayoob

et al. 2011

Neuron

145

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“…A similar mechanism has been suggested for Slit1 in the developing thalamus (Bagri et al, 2002). Recent studies also indicate a role for Slit-Robo signaling in dorsoventral positioning of longitudinal dopaminergic axons in mice (Farmer et al, 2008; Kastenhuber et al, 2009; Mastick et al, 2010) and TPOC axons in the zebrafish forebrain (Devine and Key, 2008). In the latter model, a Robo2-mediated Slit1a signal appears to promote axon spreading at the caudal diencephalon, followed by additional signals mediated by Robo1 and Robo3 that prevent further spreading in this region and in the midbrain.…”

Section: Discussionmentioning

confidence: 99%

Slit‐Robo signals regulate pioneer axon pathfinding of the tract of the postoptic commissure in the mammalian forebrain

Ricaño-Cornejo

Altick

García-Peña

et al. 2011

J of Neuroscience Research

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During early vertebrate forebrain development, pioneer axons establish a symmetrical scaffold descending longitudinally through the rostral forebrain, thus forming the tract of the postoptic commissure (TPOC). In mouse embryos, this tract begins to appear at embryonic day 9.5 (E9.5) as a bundle of axons tightly constrained at a specific dorsoventral level. We have characterized the participation of the Slit chemorepellants and their Robo receptors in the control of TPOC axon projection. In E9.5–E11.5 mouse embryos, Robo1 and Robo2 are expressed in the nucleus origin of the TPOC (nTPOC), and Slit expression domains flank the TPOC trajectory. These findings suggested that these proteins are important factors in the dorsoventral positioning of the TPOC axons. Consistently with this role, Slit2 inhibited TPOC axon growth in collagen gel cultures, and interfering with Robo function in cultured embryos induced projection errors in TPOC axons. Moreover, absence of both Slit1 and Slit2 or Robo1 and Robo2 in mutant mouse embryos revealed aberrant TPOC trajectories, resulting in abnormal spreading of the tract and misprojections into both ventral and dorsal tissues. These results reveal that Slit-Robo signaling regulates the dorsoventral position of this pioneer tract in the developing forebrain.

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“…Within this region, LOC769250 is a predicted gene with no clear function, and ROBO1 and ROBO2 belong to the ROBO family, which are members of the immunoglobulin superfamily [23]. The encoded products of both genes are integral membrane protein receptors for the SLIT-family, and have similar functions in axon guidance and migration of neuronal precursor cells [24-27]. Many molecules, such as Semaphorin 7A, Neurokinin A and Plexin-B2, participate in nervous system and immune system processes [28-32].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study of antibody response to Newcastle disease virus in chicken

Luo

et al. 2013

BMC Genet

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BackgroundSince the first outbreak in Indonesia in 1926, Newcastle disease has become one of the most common and contagious bird diseases throughout the world. To date, enhancing host antibody response by vaccination remains the most efficient strategy to control outbreaks of Newcastle disease. Antibody response plays an important role in host resistance to Newcastle disease, and selection for antibody response can effectively improve disease resistance in chickens. However, the molecular basis of the variation in antibody response to Newcastle disease virus (NDV) is not clear. The aim of this study was to detect genes modulating antibody response to NDV by a genome-wide association study (GWAS) in chickens.ResultsTo identify genes or chromosomal regions associated with antibody response to NDV after immunization, a GWAS was performed using 39,833 SNP markers in a chicken F2 resource population derived from a cross between two broiler lines that differed in their resistance. Two SNP effects reached 5% Bonferroni genome-wide significance (P<1.26×10-6). These two SNPs, rs15354805 and rs15355555, were both on chicken (Gallus gallus) chromosome 1 and spanned approximately 600 Kb, from 100.4 Mb to 101.0 Mb. Rs15354805 is in intron 7 of the chicken Roundabout, axon guidance receptor, homolog 2 (ROBO2) gene, and rs15355555 is located about 243 Kb upstream of ROBO2. Rs15354805 explained 5% of the phenotypic variation in antibody response to NDV, post immunization, in chickens. Rs15355555 had a similar effect as rs15354805 because of its linkage disequilibrium with rs15354805 (r2=0.98).ConclusionThe region at about 100 Mb from the proximal end of chicken chromosome 1, including the ROBO1 and ROBO2 genes, has a strong effect on the antibody response to the NDV in chickens. This study paves the way for further research on the host immune response to NDV.

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Longitudinal axons are guided by Slit/Robo signals from the floor plate

Cited by 18 publications

References 29 publications

A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS

A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS

Slit‐Robo signals regulate pioneer axon pathfinding of the tract of the postoptic commissure in the mammalian forebrain

Genome-wide association study of antibody response to Newcastle disease virus in chicken

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