Longitudinal Determination of Serum Placental Protein 13 during Development of Preeclampsia

Abstract

Objective: To determine maternal serum placental protein 13 (PP13) in normal pregnancy and preeclampsia. Methods: A prospective, longitudinal study with 41 normal pregnant women, 18 cases with preterm delivery or cervix insufficiency and 4 with developing late-onset preeclampsia. Six hundred and sixty-six maternal blood samples were obtained every 2–4 weeks starting at 5–8 weeks gestation (10–12 samples/patient) and tested for serum PP13 by ELISA. Results: In normal pregnant women delivering at term, median maternal serum PP13 levels were growing from 166 to 202 pg/ml and 382 pg/ml in the first, second and third trimester, respectively. Preeclamptic women had significantly reduced PP13 levels in the first trimester (multiples of median of 0.14 at 7–8 weeks; p = 0.005 compared to normal). PP13 in the third trimester was significantly higher compared to normal at 35–36 weeks with PP13 multiples of median of 1.79. Conclusion: This preliminary study indicates that low levels of PP13 in early pregnancy identify at-risk pregnancies, whereas high levels precede the syndrome in late pregnancy and suggest syncytiotrophoblast necrosis.

The relationship between maternal blood group and preeclampsia

“…13 Compared with O group, A, B, and AB groups are associated with an increased risk of thrombotic events, although this relationship is debated. ABO blood groups may differ in the occurrence of known vascular risk factors for preeclampsia, such as endothelial dysfunction 14 , insulin resistance, 15 and hypercholesterolemia. 16 ABO blood group is a key determinant of coagulation factor VIII and von Willebrand factor plasma concentrations.…”

Prediction of Preeclampsia – A Workshop Report

“…The existence of SNPs in the LGALS13 gene and of shorter PP13 isoforms, which are not recognized by the current PP13 ELISA test, might explain the lower values of PP13 in the first trimester of pregnant women who subsequently develop preeclampsia [6].…”

“…Renate Hillermann reported the identification of single nucleotide polymorphisms (SNPs) in the LGALS13 gene, which encodes placental protein 13 (PP13), a galectin expressed predominantly by the placenta [5][6]. Recent studies have demonstrated that patients who subsequently developed preeclampsia had lower maternal serum PP13 concentrations in the first trimester than women who had a normal pregnancy outcome [2,6].…”

“…Recent studies have demonstrated that patients who subsequently developed preeclampsia had lower maternal serum PP13 concentrations in the first trimester than women who had a normal pregnancy outcome [2,6].…”

Präeklampsie, Pathogenese und Vorhersage

“…Allerdings gelang die auf dem Verhältnis zwischen anti-und proangiogenetischen Faktoren basierende Vorhersage erst einige Wochen vor dem Auftreten von klinischen Symptomen. Die Aufmerksamkeit hat sich deshalb vermehrt auf die Suche nach Biomarkern im Zusammenhang mit Störungen bei der Implantation gerichtet, um durch eine frühe-re Vorhersage die Zeitspanne für eine effektive medikamentöse Prophylaxe zu erweitern [15,16].…”