Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp. and Salmonella enterica in sheep

Yang, Rongchang; Jacobson, Caroline; Gardner, G.E.; Carmichael, Ian; Campbell, Angus; Ryan, Una

doi:10.1016/j.tvjl.2014.08.001

Cited by 15 publications

(28 citation statements)

References 39 publications

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“…Newport was identified as the predominant serovar in our study regardless of the sample type, whereas S. Typhimurium and S. Kentucky were isolated at lower rates. This finding is in contrast to previous reports indicating S. Typhimurium to be either the predominant (Kidanemariam et al, 2010;Molla et al, 2006;Moussa et al, 2012;Stipetic et al, 2016;Tadesse, 2015;Wani et al, 2013;Yang et al, 2014) or the second-most dominant (Lewerin et al, 2011;Tadesse and Tessema, 2014) serovar isolated. There are only a few reports that mention S. Newport as the second-most dominant serovar and one of the lesser isolated serovars in sheep FC (Dargatz et al, 2015;Stipetic et al, 2016;USDA, 2013).…”

Section: Discussioncontrasting

confidence: 99%

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Çetin

Temelli

Eyigör

2020

Food Sci Anim Resour

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This study aimed to determine the current prevalence, serovar distribution and antimicrobial resistance rate and patterns of nontyphoid Salmonella (NTS) in slaughter sheep and their edible offal. While filling the gap of up to date related information in Turkey, data presented is also of significance since contamination of ovine meat, its products and offal with this pathogen is threat to public health due to their considerably high consumption rates in our country. Current NTS carriage in 200 apparently healthy slaughter sheep by ISO 6579:2002, 6579:2002/A1:2007 standard bacteriology (ISO) was 5% (10/200) (4 fecal content -2%, 3 mesenterial lymph node -1.5%, 3 kidney -1.5%) out of 1,400 samples (0.7%), with no isolation from carcass, liver, gallbladder, spleen. Realtime PCR was in substantial agreement to ISO in confirming Salmonella-suspect isolates (Relative Trueness: 93.6%). S. Newport (40%) was the predominant serovar, followed by the second prevalent serovars as S. Typhimurium and S. Kentucky (20%), and by S. Umbilo and S. Corvallis (10%). Four and 6 out of 10 NTS isolates were susceptible (40%) and resistant (60%) to 18 antimicrobials, respectively. S. Typhimurium isolates were multidrug resistant (MDR) to tigecycline and sulphamethoxazole/trimethoprim, with one also resistant to cefepime. S. Corvallis was MDR to ampicillin, ciprofloxacin, norfloxacin and pefloxacin. The predominance of S. Newport and first isolation of S. Corvallis in sheep in the world; first time isolations of Newport, Kentucky, Corvallis, Umbilo serovars from sheep in Turkey; and high antimicrobial resistance rates obtained in majority of the isolates highlights study findings.

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Section: Discussioncontrasting

confidence: 99%

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Çetin

Temelli

Eyigör

2020

Food Sci Anim Resour

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“…Primers and probes targeting the Campylobacter spp. purine biosynthesis gene ( purA ) and S. enterica outer membrane protein ( ompF ) were as previously described . Primers and probes for E. coli O157/O145 were designed using software supplied by Biosearch Technologies (Petaluma, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, we developed and validated a quantitative multiplex PCR (qPCR) for detecting and enumerating Salmonella and Campylobacter in sheep faecal samples . In the present study, we expanded this multiplex qPCR to include E. coli O157/O147 and then used this qPCR assay to assess the prevalence and shedding of E. coli O157/O145, Salmonella and Campylobacter in sheep in sale yards and abattoir effluent in Western Australia.…”

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confidence: 99%

Prevalence and pathogen load of Campylobacter spp., Salmonella enterica and Escherichia coliO157/O145 serogroup in sheep faeces collected at sale yards and in abattoir effluent in Western Australia

et al. 2017

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The overall prevalences of Campylobacter, S. enterica and E. coli O157/O145 were generally low (<6%), but point prevalences ranged considerably in healthy sheep (up to 26% for E. coli O157/O145). Further work to determine risk factors for shedding of bacterial organisms in meat sheep in the pre-slaughter period (on-farm, sale yards and lairage at abattoirs) could further reduce the risk of contamination of meat products.

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“…Ainda sobre os problemas reprodutivos, alguns autores já afirmaram que as taxas de eliminação de Campylobacter spp. podem tornar-se elevadas devido ao estresse ou a infecções múltiplas, que reduzem a imunidade dos animais (Stanley & Jones 2003, Yang et al 2014. Jones et al (1999) observaram que quantidades mais elevadas de eliminação da bactéria coincidiam com a parição, desmame e troca de pasto, como também as ovelhas que não estavam eliminando Campylobacter spp.…”

Section: Resultsunclassified

Ocorrência de ovinos portadores da infecção por Campylobacter spp. no estado de Pernambuco

et al. 2018

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RESUMO: Objetivou-se com este estudo determinar a ocorrência e os fatores de risco associados à infecção por Campylobacter spp. em criações de ovinos no estado de Pernambuco, Brasil. Foram coletadas 421 amostras fecais de ovinos procedentes de 20 rebanhos para o isolamento de Campylobacter spp. As espécies Campylobacter fetus subsp. fetus e Campylobacter jejuni foram identificadas por Reação em Cadeia da Polimerase (PCR). Para análise dos fatores de risco foi realizada uma análise univariada e posteriormente regressão logística a partir de questionário com perguntas objetivas sobre o manejo higiênico-sanitário e reprodutivo. A ocorrência para Campylobacter spp. foi de 4,5% (19/421; I.C. 2,8% - 7,1%). Das 19 amostras positivas no cultivo, oito (1,9%; I.C. 0,9% - 3,9%) foram classificadas como C. fetus subsp. fetus e sete (1,7%; I.C. 0,7% - 3,6%) como C. jejuni, com co-infecção em quatro amostras (0,95%). O número de focos identificados foi de 35,0% (7/20) das criações de ovinos que apresentavam pelo menos um animal positivo. Na análise de regressão logística não foi identificada nenhuma das variáveis como fator de risco. Este é o primeiro registro da infecção por Campylobacter spp. em rebanhos ovinos no Nordeste do Brasil, concluindo-se que a infecção ocorre nesses rebanhos. Dessa forma, se faz necessário à implementação de medidas de controle e prevenção, para impedir a propagação do agente entre as criações, evitando prejuízos para ovinocultura e riscos para saúde pública, uma vez que a campilobacteriose é considerada uma zoonose emergente.

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Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp. and Salmonella enterica in sheep

Abstract: Longitudinal prevalence, faecal shedding and molecular characterisation of campylobacter spp. and salmonella enterica in sheep, The Veterinary Journal (2014), http://dx.doi.org

Cited by 15 publications

References 39 publications

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Prevalence and pathogen load of Campylobacter spp., Salmonella enterica and Escherichia coliO157/O145 serogroup in sheep faeces collected at sale yards and in abattoir effluent in Western Australia

Ocorrência de ovinos portadores da infecção por Campylobacter spp. no estado de Pernambuco

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