Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces

Olsen, Timothy R.; Casco, Megan; Herbst, Austin; Evans, Grace; Rothermel, Taylor M.; Pruett, Lauren; Reid, Jared J.; Barry, Kelly; Jaeggli, Michael; Simionescu, Dan T.; Visconti, Richard P.; Alexis, Frank

doi:10.3390/bioengineering3040029

Cited by 8 publications

(3 citation statements)

References 50 publications

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“…However, dense collagenic tissue is necessary to obtain sufficient mechanical properties. They finally improved their process by adding a cyclic longitudinal stretching force using rod magnets (cyclic longitudinal translation at 1 Hz with a 10% magnitude [65]. This postprocess aimed to stimulate ECM deposition by cells during a third maturation step (three to seven days) and consequently enhance mechanical properties.…”

Section: Cell Aggregate Assembly Approachesmentioning

confidence: 99%

Current biofabrication methods for vascular tissue engineering and an introduction to biological textiles

Kawecki

L’Heureux

2023

Biofabrication

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Cardiovascular diseases are the leading cause of mortality in the world and encompass several important pathologies, including atherosclerosis. In the cases of severe vessel occlusion, surgical intervention using bypass grafts may be required. Synthetic vascular grafts provide poor patency for small diameter applications (< 6 mm) but are widely used for hemodialysis access and, with success, larger vessel repairs. In very small vessels, such as coronary arteries, synthetics outcomes are unacceptable, leading to the exclusive use of autologous (native) vessels despite their limited availability and, sometimes, quality. Consequently, there is a clear clinical need for a small-diameter vascular graft that can provide outcomes similar to native vessels. Many tissue-engineering approaches have been developed to offer native-like tissues with the appropriate mechanical and biological properties in order to overcome the limitations of synthetic and autologous grafts. This review overviews current scaffold-based and scaffold-free approaches developed to biofabricate tissue-engineered vascular grafts (TEVGs) with an introduction to the biological textile approaches. Indeed, these assembly methods show a reduced production time compared to processes that require long bioreactor-based maturation steps. Another advantage of the textile-inspired approaches is that they can provide better directional and regional control of the TEVG mechanical properties.

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Section: Cell Aggregate Assembly Approachesmentioning

confidence: 99%

Current biofabrication methods for vascular tissue engineering and an introduction to biological textiles

Kawecki

L’Heureux

2023

Biofabrication

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“…All arteries experience longitudinal (axial) stresses varying from 40% to 65% in vivo [ 159 ]. Physiological alterations can alter these longitudinal stresses because arterial tethering exposed by surrounding tissues maintains them.…”

Section: Mechanical Forces Acting On the Vascular Graftsmentioning

confidence: 99%

An Investigation of the Constructional Design Components Affecting the Mechanical Response and Cellular Activity of Electrospun Vascular Grafts

et al. 2022

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Cardiovascular disease is anticipated to remain the leading cause of death globally. Due to the current problems connected with using autologous arteries for bypass surgery, researchers are developing tissue-engineered vascular grafts (TEVGs). The major goal of vascular tissue engineering is to construct prostheses that closely resemble native blood vessels in terms of morphological, mechanical, and biological features so that these scaffolds can satisfy the functional requirements of the native tissue. In this setting, morphology and cellular investigation are usually prioritized, while mechanical qualities are generally addressed superficially. However, producing grafts with good mechanical properties similar to native vessels is crucial for enhancing the clinical performance of vascular grafts, exposing physiological forces, and preventing graft failure caused by intimal hyperplasia, thrombosis, aneurysm, blood leakage, and occlusion. The scaffold’s design and composition play a significant role in determining its mechanical characteristics, including suturability, compliance, tensile strength, burst pressure, and blood permeability. Electrospun prostheses offer various models that can be customized to resemble the extracellular matrix. This review aims to provide a comprehensive and comparative review of recent studies on the mechanical properties of fibrous vascular grafts, emphasizing the influence of structural parameters on mechanical behavior. Additionally, this review provides an overview of permeability and cell growth in electrospun membranes for vascular grafts. This work intends to shed light on the design parameters required to maintain the mechanical stability of vascular grafts placed in the body to produce a temporary backbone and to be biodegraded when necessary, allowing an autologous vessel to take its place.

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“…Other similar magnetic beads‐based methods are pursued in academic environments. Relevant to this discussion are the Janus'‐like microbeads which are internalized and were demonstrated to assist the formation of vascular‐relevant structures . While these methods are definitely useful for in vitro models and experimentation, their translation to clinical practice is more questionable, particularly due to the limited size of the constructs and the residual magnetic material.…”

Section: Recent Developments In Cardiovascular Scaffold‐free 3d Bioprmentioning

confidence: 99%

Progress in scaffold‐free bioprinting for cardiovascular medicine

Moldovan

2018

J Cellular Molecular Medi

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Biofabrication of tissue analogues is aspiring to become a disruptive technology capable to solve standing biomedical problems, from generation of improved tissue models for drug testing to alleviation of the shortage of organs for transplantation. Arguably, the most powerful tool of this revolution is bioprinting, understood as the assembling of cells with biomaterials in three‐dimensional structures. It is less appreciated, however, that bioprinting is not a uniform methodology, but comprises a variety of approaches. These can be broadly classified in two categories, based on the use or not of supporting biomaterials (known as “scaffolds,” usually printable hydrogels also called “bioinks”). Importantly, several limitations of scaffold‐dependent bioprinting can be avoided by the “scaffold‐free” methods. In this overview, we comparatively present these approaches and highlight the rapidly evolving scaffold‐free bioprinting, as applied to cardiovascular tissue engineering.

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Longitudinal Stretching for Maturation of Vascular Tissues Using Magnetic Forces

Cited by 8 publications

References 50 publications

Current biofabrication methods for vascular tissue engineering and an introduction to biological textiles

Current biofabrication methods for vascular tissue engineering and an introduction to biological textiles

An Investigation of the Constructional Design Components Affecting the Mechanical Response and Cellular Activity of Electrospun Vascular Grafts

Progress in scaffold‐free bioprinting for cardiovascular medicine

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