Longitudinal study of sperm DNA fragmentation as measured by terminal uridine nick end-labelling assay

Sergerie, Martin; Laforest, G.; Boulanger, Kyle; Bissonnette, François; Bleau, G.

doi:10.1093/humrep/deh885

Cited by 56 publications

(35 citation statements)

References 37 publications

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“…We also found that the variability of sDF was lower than that of any standard semen parameter, and lower than what had been previously reported ($30%) for similar periods with the use of both SCSA (36,37) and TUNEL (38). Such a difference could be due to the recruitment criteria adopted in our study that, at variance with the above studies (36)(37)(38), excluded any conditions among those that so far are known to affect sDF (including recent pharmacologic therapies and fever episodes [28,29]).…”

Section: Figurecontrasting

confidence: 62%

“…Such a difference could be due to the recruitment criteria adopted in our study that, at variance with the above studies (36)(37)(38), excluded any conditions among those that so far are known to affect sDF (including recent pharmacologic therapies and fever episodes [28,29]). Indeed, when some of these conditions are excluded, the variability of sDF results decreased ($20% [39]).…”

Section: Figurementioning

confidence: 99%

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DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters

Muratori

Marchiani

Tamburrino

et al. 2015

Fertility and Sterility

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Objective: To evaluate whether sperm DNA fragmentation (sDF), measured in brighter, dimmer, and total populations, predicts natural conception, and to evaluate the intra-individual variability of sDF. Design: Prospective study. Setting: Outpatient clinic and diagnostic laboratory. Patient(s): A total of 348 unselected patients and 86 proven fertile men. Intervention(s): None. Main Outcome Measure(s): sDF was revealed with the use of terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL)/propidium iodide (PI). Receiver operating characteristic (ROC) curves were built before and after matching fertile men to patients for age (76:152) or semen parameters (68:136) or both (49:98). Intra-individual variability of sDF was assessed over 2 years. Result(s): Brighter (area under ROC curve [AUC] 0.718 AE 0.54), dimmer (AUC 0.655 AE 0.63), and total (AUC 0.757 AE 0.54) sDF predict male fertility in unmatched and age-or semen parameters-matched subjects. After matching for both age and semen parameters, only brighter (AUC 0.711 AE 0.83) and total (AUC 0.675 AE 0.92) sDF predict male fertility. At high values of total sDF, brighter predicts natural conception better than total sDF. Intra-individual coefficients of variation of sDF were 9.2 AE 8.6% (n ¼ 25), 12.9 AE 12.7% (n ¼ 53), and 14.0 AE 12.6% (n ¼ 70) over, respectively, 100-day and 1-and 2-year periods, appearing to be the most stable of the evaluated semen parameters. Conclusion(s):The predictive power of total sDF partially depends on age and semen parameters, whereas brighter sDF independently predicts natural conception. Therefore, brighter sDF is a fraction of sDF that adds new information to the routine semen analysis. At high levels of sDF, distinguishing the two sperm populations improves the predictive power of sDF. Overall, our results support the idea that TUNEL/PI can be of clinical usefulness in the male fertility workup. (Fertil Steril Ò 2015;104:582-90. Ó2015 by American Society for Reproductive Medicine.)

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Section: Figurecontrasting

confidence: 62%

Section: Figurementioning

confidence: 99%

DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters

Muratori

Marchiani

Tamburrino

et al. 2015

Fertility and Sterility

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show abstract

“…Therefore, it should have a higher predictive value than other tests that measure "potential" DNA damage. This is further supported by recent studies which use the TUNEL assay to determine sperm DNA fragmentation and demonstrate a highly predicative value for male fertility in natural conception with 96.5% sensitivity and 89.4% specificity and good stability [16,36]. The second one is the SCSA, often referred as being able to reflect only "potential" DNA damage.…”

Section: Introductionmentioning

confidence: 63%

Correlation of sperm DNA damage with IVF and ICSI outcomes: A systematic review and meta-analysis

Wang

Cai

et al. 2006

J Assist Reprod Genet

163

110

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Our meta-analysis indicates that sperm DNA damage, as assessed by the TUNEL assay, significantly decreases only the chance of IVF clinical pregnancy, but not that of either IVF fertilization or ICSI fertilization or ICSI clinical pregnancy. Besides, our results also reveal that sperm DNA damage, when assessed by the SCSA, has no significant effect on the chance of clinical pregnancy after IVF or ICSI treatment.

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“…Standard seminal parameters of sperm concentration, motility, and normal morphology can be highly variable over time within individuals [4,6,82]. In comparison, measures of sperm DNA damage has been shown to have good stability over time within individual with TUNEL [110,111] and SCSA assays [39,127,130]. Earlier studies have reported low coefficients of variation (CV) of 10% [39] and 21 % [127,130] of DFI with the SCSA.…”

Section: Sperm Chromatin Dispersion Testmentioning

confidence: 99%

“…These studies utilized a variety of different assays to analyze sperm DNA integrity but consistently showed that infertile men have a significantly higher level of sperm DNA damage when compared with fertile men. A threshold value of 20% sperm DNA fragmentation with TUNEL assay has been suggested to distinguish between fertile controls and infertile men [110,111]. Additionally, ≥30% or >40% DFI as measured by the SCSA has also been suggested as specific threshold levels, above which the chance of pregnancy approaches zero [40,114].…”

Section: In Vivo Fertilizationmentioning

confidence: 99%

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

Schulte

Ohl

Sigman³

et al. 2009

J Assist Reprod Genet

227

159

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Male factor infertility is the sole cause of infertility in approximately 20% of infertile couples, with an additional 30% to 40% secondary to both male and female factors. Current means of evaluation of male factor infertility remains routine semen analysis including seminal volume, pH, sperm concentration, motility, and morphology. However, approximately 15% of patients with male factor infertility have a normal semen analysis and a definitive diagnosis of male infertility often cannot be made as a result of routine semen analysis. Attention has focused on the role of sperm nuclear DNA integrity in male factor infertility. Here we review the structure of human sperm chromatin, the etiology and mechanisms of sperm DNA damage, current tests available to assess sperm DNA integrity, and effect of sperm DNA integrity on reproductive outcomes.

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Longitudinal study of sperm DNA fragmentation as measured by terminal uridine nick end-labelling assay

Abstract: This longitudinal study shows that sperm DNA fragmentation is a parameter with good stability (repeatability) over time; it can be taken as a baseline both in healthy fertile men and in patients from infertility couples.

Cited by 56 publications

References 37 publications

DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters

DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters

Correlation of sperm DNA damage with IVF and ICSI outcomes: A systematic review and meta-analysis

Sperm DNA damage in male infertility: etiologies, assays, and outcomes

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