Longitudinal Tracking of Human Fetal Cells Labeled with Super Paramagnetic Iron Oxide Nanoparticles in the Brain of Mice with Motor Neuron Disease

Bigini, Paolo; Diana, Valentina; Barbera, Sara; Fumagalli, Elena; Micotti, Edoardo; Sitia, Leopoldo; Paladini, Alessandra; Bisighini, Cinzia; Grada, L. De; Coloca, Laura; Colombo, Laura; Manca, Pina; Bossolasco, Patrizia; Malvestiti, Francesca; Fiordaliso, Fabio; Forloni, Gianluigi; Morbidelli, Massimo; Salmona, Mario; Giardino, Daniela; Mennini, Tiziana; Moscatelli, Davide; Silani, Vincenzo; Cova, Lidia

doi:10.1371/journal.pone.0032326

Cited by 31 publications

(50 citation statements)

References 36 publications

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“…[12][13][14]20,22,24,31,32,65,[66][67][68][69][70][71]74 In addition to dextran, seven other types of SPION coatings were identified, such as starch, phosphatidylcholine, DEAE-diphosphate starch and poly-diallyl-dimethylamine in the Riegler et al 72 study; carboxylated iron oxide core was seen in the Detante et al 64 study, silica in the study by Wang et al 63 monomer citrate in the study by Stroh et al 23 and DPPA in the study by Bjorklund et al 19 There was one study 62 that used both dextran and chitosan.…”

Section: Resultsmentioning

confidence: 99%

“…However, in ALS the situation is more dire, because on average patients die 4 years after being diagnosed. 28 Functional improvement was seen in preclinical [29][30][31][32][33] and clinical studies 34 after cell therapy.…”

Section: Parkinson's Diseasementioning

confidence: 99%

“…Regarding the analysis of the SPION cell labeling process by the 32 selected articles, the majority (19) used mesenchymal stem cells (MSC), six articles 12,19,23,33,66,74 used embryonic stem cells, five articles 20,25,30,70,76 used neural stem cells, one article 65 used hematopoietic stem cells, and the other article 31 used amniotic fluid-derived stem cells ( Table 2). The use of a transfection agent and a culture medium to optimize the stem cell labeling process with SPION is usual.…”

Section: In Vitro Experiments For Stem Cell Labelingmentioning

confidence: 99%

“…Only five studies [30][31][32][33][34] (Table 2) were selected that used cells from different sources, such as: stem cells derived from human skeletal muscle -SkmSCs, 30 bone marrow derived human MSC, 34 amniotic fluid derived from human stem cells, 32 human embrionary stem cells, 33 and neural rat stem cells. 31 To analyze the culture mediums, two studies 32,34 used basic DMEM, and one study 30 supplemented the medium with epidermal growth factor (EGF; 20 ng/mL), basic fibroblast growth factor (bFGF; 10 ng/mL), neural stem cell growth N2 and Noggin (100 ng/mL), and Kim et al 33 used only DMEM supplemented with stem cell N2.…”

Section: Multiple Sclerosis and Alsmentioning

confidence: 99%

“…31 To analyze the culture mediums, two studies 32,34 used basic DMEM, and one study 30 supplemented the medium with epidermal growth factor (EGF; 20 ng/mL), basic fibroblast growth factor (bFGF; 10 ng/mL), neural stem cell growth N2 and Noggin (100 ng/mL), and Kim et al 33 used only DMEM supplemented with stem cell N2.…”

Section: Multiple Sclerosis and Alsmentioning

confidence: 99%

See 4 more Smart Citations

Therapeutics with SPION-labeled stem cells for the main diseases related to brain aging: a systematic review

Alvarim¹,

Nucci²,

Mamani³

et al. 2014

IJN

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The increase in clinical trials assessing the efficacy of cell therapy for structural and functional regeneration of the nervous system in diseases related to the aging brain is well known. However, the results are inconclusive as to the best cell type to be used or the best methodology for the homing of these stem cells. This systematic review analyzed published data on SPION (superparamagnetic iron oxide nanoparticle)-labeled stem cells as a therapy for brain diseases, such as ischemic stroke, Parkinson’s disease, amyotrophic lateral sclerosis, and dementia. This review highlights the therapeutic role of stem cells in reversing the aging process and the pathophysiology of brain aging, as well as emphasizing nanotechnology as an important tool to monitor stem cell migration in affected regions of the brain.

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Section: Resultsmentioning

confidence: 99%

Section: Parkinson's Diseasementioning

confidence: 99%

Section: In Vitro Experiments For Stem Cell Labelingmentioning

confidence: 99%

Section: Multiple Sclerosis and Alsmentioning

confidence: 99%

Section: Multiple Sclerosis and Alsmentioning

confidence: 99%

See 3 more Smart Citations

Therapeutics with SPION-labeled stem cells for the main diseases related to brain aging: a systematic review

Alvarim¹,

Nucci²,

Mamani³

et al. 2014

IJN

View full text Add to dashboard Cite

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Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity

Valdiglesias

Kılıç

Costa

et al. 2014

Environ and Mol Mutagen

155

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Iron oxide nanoparticles (ION) with superparamagnetic properties hold great promise for use in various biomedical applications; specific examples include use as contrast agents for magnetic resonance imaging, in targeted drug delivery, and for induced hyperthermia cancer treatments. Increasing potential applications raise concerns over their potential effects on human health. Nevertheless, very little is currently known about the toxicity associated with exposure to these nanoparticles at different levels of biological organization. This article provides an overview of recent studies evaluating ION cytotoxicity, genotoxicity, developmental toxicity and neurotoxicity. Although the results of these studies are sometimes controversial, they generally indicate that surface coatings and particle size seem to be crucial for the observed ION-induced effects, as they are critical determinants of cellular responses and intensity of effects, and influence potential mechanisms of toxicity. The studies also suggest that some ION are safe for certain biomedical applications, while other uses need to be considered more carefully. Overall, the available studies provide insufficient evidence to fully assess the potential risks for human health related to ION exposure. Additional research in this area is required including studies on potential long-term effects.

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Magnetic‐directed patterning of cell spheroids

Whatley

Zhang

et al. 2013

J Biomedical Materials Res

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We have described an approach to fabricate three-dimensional (3D) cell-based structures using functionalized super paramagnetic iron oxide nanoparticles (SPIONs) as patterning agents to guide the assembly of endothelial cell spheroids into 3D patterns using the magnetic forces generated by a prefabricated magnetic template. SPIONs were first uptaken by endothelial cells before they were assembled into uniform-sized spheroids through a home-made robotic spheroid maker. To guide the magnetic spheroids, a unique magnetic template was fabricated using computer-aided design and cut from a magnetic sheet. The spheroids were then guided to the prefabricated magnetic template through the attractive magnetic forces between the SPIONs inside the endothelial cells and the magnetic template. Fusion of endothelial cell spheroids over time while adhered to the magnetic template allowed for the formation of 3D cell-based structures. Subsequent removal of the prefabricated magnetic template left 3-D endothelial cell sheets, which may be stacked to fabricate complicated 3D multicellular tissue structures. To enhance the cytocompatibility, SPIONs were silica-coated before use. At low concentrations, the SPIONs did not adversely affect cell viability, proliferation, and phenotype stability. Light and confocal microscopy showed that endothelial cell spheroids could be reproducibly created with high uniformity. The endothelial cells were able to remain viable and maintain the 3D structure in vitro. We have proved the concept to use SPIONs as a patterning agent to direct the attachment and self assembly of SPION-loaded endothelial cell spheroids on a prefabricated magnetic template for the formation of 3D cell based structures. A magnetic-directed technique allows quick patterning of cell spheroids in accordance with desirable magnetic patterns, therefore, holding promise for scalable fabrication of complicated 3D multicellular tissue structures. By varying the cell types and the prefabricated magnetic patterns, this magnetic-directed patterning strategy may find use in bioprinting and multicellular tissue graft fabrication.

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Longitudinal Tracking of Human Fetal Cells Labeled with Super Paramagnetic Iron Oxide Nanoparticles in the Brain of Mice with Motor Neuron Disease

Cited by 31 publications

References 36 publications

Therapeutics with SPION-labeled stem cells for the main diseases related to brain aging: a systematic review

Therapeutics with SPION-labeled stem cells for the main diseases related to brain aging: a systematic review

Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity

Magnetic‐directed patterning of cell spheroids

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