Methods for identifying origin, movement, and foraging
areas of
animals are essential for understanding ecosystem connectivity, nutrient
flows, and other ecological processes. Telemetric methods can provide
detailed spatial coverage but are limited to a minimum body size of
specimen for tagging. In recent years, stable isotopes have been increasingly
used to track animal migration by linking landscape isotope patterns
into movement (isoscapes). However, compared to telemetric methods,
the spatial resolution of bulk stable isotopes is low. Here, we examined
a novel approach by evaluating the use of compound-specific hydrogen
and carbon stable isotopes of fatty acids (δ
2
H
FA
and δ
13
C
FA
) from fish liver,
muscle, brain, and eye tissues for identifying site specificity in
a 254 km
2
sub-alpine river catchment. We analyzed 208 fish
(European bullhead, rainbow trout, and brown trout) collected in 2016
and 2018 at 15 different sites. δ
13
C
FA
values of these fish tissues correlated more among each other than
those of δ
2
H
FA
values. Both δ
2
H
FA
and δ
13
C
FA
values
showed tissue-dependent isotopic fractionation, while fish taxa had
only small effects. The highest site specificity was for δ
13
C
DHA
values, while the δ
2
H isotopic
difference between linoleic acid and alpha-linolenic acid resulted
in the highest site specificity. Using linear discrimination analysis
of FA isotope values, over 90% of fish could be assigned to their
location of origin; however, the accuracy dropped to about 56% when
isotope data from 2016 were used to predict the sites for samples
collected in 2018, suggesting temporal shifts in site specificity
of δ
2
H
FA
and δ
13
C
FA
. However, the predictive power of δ
2
H
FA
and δ
13
C
FA
over this time interval
was still higher than site specificity of bulk tissue isotopes for
a single time point. In summary, compound-specific isotope analysis
of fatty acids may become a highly effective tool for assessing fine
and large-scale movement and foraging areas of animals.