Longterm survival of transplanted human corneal epithelial cells and corneal stem cells

Egarth, Maria; Hellkvist, Josefine; Claesson, Margareta; Hanson, Charles; Stenevi, Ulf

doi:10.1111/j.1395-3907.2005.00485.x

Cited by 21 publications

(14 citation statements)

References 20 publications

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“…Sampling the recipients of a gendermismatched (male-to-female) allo-SCT enables easy separation of donor-and recipient-type cells by means of sexchromosome-specific fluorescence in situ hybridization (FISH), a method previously employed on ocular tissue by Wollensak & Green (1999), Stenevi et al (2002) and Egardt et al (2005). The aim of this study was to identify and characterize cells of donor origin on the ocular surfaces of allografted patients.…”

Section: Introductionmentioning

confidence: 99%

Donor‐derived myofibroblasts in the ocular surface after allogeneic haematopoietic stem cell transplantation

Hallberg

Wernstedt

Hanson

et al. 2006

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To identify and characterize cells of donor origin in the ocular surface of female recipients who have undergone allogeneic haematopoietic stem cell transplantation (allo-SCT) from a male donor. Methods: Cytological impressions from the eyes of nine allografted patients (17 eyes) were analysed. Donor cells were identified using sex-chromosomespecific fluorescence in situ hybridization (FISH). Cells were characterized by immunohistochemistry (IHC) using the CK3 and CK19 epithelial markers, the panleucocytic marker CD45 and the myofibroblast marker a-SMA. Results: No epithelial cells of donor origin were observed in the corneal or conjunctival samples. Cells of donor origin were found in the corneal samples, although these were often too degraded to allow characterization by IHC. In the conjunctiva, a median of 86% of the total number of cells were of recipient origin, including a subgroup (2%) of giant cells exhibiting polyploidy (range 4-18 n), found in the limbal region. Donor cells were detected in the conjunctiva of all nine patients at a median ratio of 9%, of which two-thirds were CD45+ + ⁄ a-SMA+. Conclusions: We observed superficially located myofibroblasts of donor origin in all allografted patients, but not in samples from healthy controls. Whether myofibroblasts are implicated in ocular graft-versus-host disease requires further studies.

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Section: Introductionmentioning

confidence: 99%

Donor‐derived myofibroblasts in the ocular surface after allogeneic haematopoietic stem cell transplantation

Hallberg

Wernstedt

Hanson

et al. 2006

Acta Ophthalmologica Scandinavica

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“…The best possible epithelial structure in donor corneas will in all likelihood reduce patient discomfort and postoperative time in hospital. Moreover, several studies have shown that after penetrating keratoplasty, donor epithelial cells reside in the transplant for a long time (Kaye 1980;Kinoshita et al 1981), sometimes for more than a year after transplantation (Egarth et al 2005). This means that the donor epithelium interacts with the recipient's epithelium after penetrating keratoplasty and this may influence the clinical outcome.…”

Section: Discussionmentioning

confidence: 99%

Donor corneas for transplantation: a scanning electron microscopic study of the epithelium

Slettedal¹,

Lyberg²,

Ramstad³

et al. 2006

Acta Ophthalmologica Scandinavica

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. Purpose: Donor corneas are processed in eye banks and used for transplantation as a standard routine. The maximum time limit post‐mortem for harvesting donor tissue varies greatly between eye banks. This study aimed to examine the corneal epithelium for structural changes post‐mortem. Methods: A total of 51 corneas harvested between 14 and 163 hours post‐mortem were examined using scanning electron and light microscopy. Results: Cell loss occurred through desquamation of flat superficial cells during the first days. In corneas with a post‐mortem time of more than 2−3 days, large superficial cell sheets and deeper cells detached, starting centrally. Deep peripheral cells remained. The loss of the superficial cells revealed the 3‐dimensional structure of the epithelium and the membrane characteristics of deeper cells. Conclusion: The longer the time post‐mortem, the greater the epithelial cell loss. However, a rim of peripheral cells remained, even after 7 days. The superficial cell layer showed signs of strong lateral attachment and broke up in a sheet‐like fashion. The intercellular adhesion between deeper cells and adhesion between the basal cells and the basement membrane appeared to be weak post‐mortem. The cell membrane structures of the remaining cells were surprisingly well retained. The clinical implication of the study is discussed.

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“…In contrast to earlier studies [1,2,3,4,6,7,8], this approach allowed identification of the distribution of donor cells in the cornea. Moreover, this method is not limited to sex-mismatched cases.…”

Section: Introductionmentioning

confidence: 90%

The Survival of Donor-Derived Cells in a Successfully Grafted Corneal Button 10 Years after Penetrating Keratoplasty for Lattice Dystrophy

et al. 2009

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Aims: To investigate the survival of donor-derived cells in a successfully grafted corneal button 10 years after penetrating keratoplasty for lattice dystrophy. Methods: In 1996, a 48-year-old male with lattice corneal dystrophy underwent penetrating keratoplasty 3 times in the right eye within a 3-month interval. Nine years and 7 months later, the patient underwent a fourth penetrating keratoplasty. After surgery, the previous graft was analyzed to determine the origin of the cells. The epithelium and endothelium were removed, and then the button was dissected into 5 stromal blocks measuring 2 × 1.8 mm. Tissues underwent forensic genotyping using 16 markers (amelogenin for sex chromosomes and 15 autosomal short tandem repeats). Patient buccal tissue DNA was used as a control. Results: The epithelium and buccal tissue contained identical DNA (i.e. recipient DNA). Similarly, the most peripheral stromal tissue contained only recipient DNA. In contrast, the most central stromal tissue only contained DNA of nonrecipient origin (presumably donor), while the stromal tissue between the periphery and center contained both recipient and nonrecipient DNA. Conclusions: The corneal stroma was infiltrated by surrounding recipient-derived keratocytes from the periphery. Therefore, more donor-derived cells had survived in the central stroma.

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Longterm survival of transplanted human corneal epithelial cells and corneal stem cells

Cited by 21 publications

References 20 publications

Donor‐derived myofibroblasts in the ocular surface after allogeneic haematopoietic stem cell transplantation

Donor‐derived myofibroblasts in the ocular surface after allogeneic haematopoietic stem cell transplantation

Donor corneas for transplantation: a scanning electron microscopic study of the epithelium

The Survival of Donor-Derived Cells in a Successfully Grafted Corneal Button 10 Years after Penetrating Keratoplasty for Lattice Dystrophy

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