Lonicera caerulea and Vaccinium myrtillus fruit polyphenols protect HaCaT keratinocytes against UVB-induced phototoxic stress and DNA damage

Svobodová, Alena Rajnochová; Zdařilová, Adéla; Vostalová, Jitka

doi:10.1016/j.jdermsci.2009.08.004

Cited by 65 publications

(48 citation statements)

References 43 publications

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“…phenolic compounds [37][38][39]. In this study, treatment of the these cell lines with extracted phenolic compounds altered cell viability, but the pattern of dose dependency was different between the two cell lines ( Table 2).…”

Section: Note: the Content Of Individual Phenolic Compounds In The Flmentioning

confidence: 84%

Edible flowers — antioxidant activity and impact on cell viability

Kuceková¹,

Mlček

Humpolíček³

et al. 2013

Open Life Sciences

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The phenolic compound composition, antioxidant activity and impact on cell viability of edible flower extracts of Allium schoenoprasum; Bellis perennis; Cichorium intybus; Rumex acetosa; Salvia pratensis; Sambucus nigra; Taraxacum officinale; Tragopogon pratensis; Trifolium repens and Viola arvensis was examined for the first time. Total phenolic content of the flowers of these plants fell between 11.72 and 42.74 mg of tannin equivalents/kg of dry matter. Antioxidant activity ranged from 35.56 to 71.62 g of ascorbic acid equivalents/kg of dry matter. Using the Human Hepatocellular Carcinoma cell-line (HepG2) and the Human Immortalized Non-tumorigenic Keratinocyte cell line (HaCaT), we assessed cell viability following a 3 day incubation period in media containing 25, 50, 75 and 100 μg/ml of total phenolic compounds using a colorimetric MTT assay. These three properties could make the herbs useful in treatment of various diseases like cancer. The tested extracts had significant effects on cell viability, but the effects were dependent not only on the phenolic compound concentration and the edible flowers species, but also on the phenolic compound and antioxidant profiles. In addition, responses differed between cell lines.

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Section: Note: the Content Of Individual Phenolic Compounds In The Flmentioning

confidence: 84%

Edible flowers — antioxidant activity and impact on cell viability

Kuceková¹,

Mlček

Humpolíček³

et al. 2013

Open Life Sciences

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“…cancer (Gruia et al, 2008), neurodegenerative diseases (Gazdik et al, 2008), their anti-inflammatory effect has been proved as well (Svobodova et al, 2009). The colour stability of anthocyanin depends on the combination of various factors such as the structure and concentration of anthocyanin, pH, temperature and presence of complexing agents (phenols, metals) or other chemical substances (Jurikova et al, 2012).…”

Section: The Evaluation Of Anthocyanin Content Of Edible Honeyberry (mentioning

confidence: 99%

Antocianinų kiekis sausmedžio (Lonicera kamtschatica) klonuose šaldymo metu, priklausomai nuo antioksidantinės ir maistinės vertės rodiklių

Juríková

̃li

Rop

et al. 2014

Zemdirbyste-Agriculture

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The aim of the study was to determine changes in anthocyanin content of 17 selected Klčov's clones of honeyberry (Lonicera kamtschatica (Sevast.) Pojark) during freezing at −18°C for 6 months and find out the relationship between anthocyanins and dry matter, sugar, organic acids and ascorbic acid content in fresh fruits by cluster analysis. Clones of honeyberry can be divided into three clusters according to dry matter, sugar, organic acids and total anthocyanin content and total antioxidant capacity. The total anthocyanin level measured in fresh samples was 5.96-19.80 mg 100 g -1 of fresh mass (FM). The scavenging effect of acidified 80% ethanol extracts on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical ranged from 30.03% to 41.13%. Anthocyanins displayed a negative weak correlation with total antioxidant capacity (R = −0.585).

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“…Furthermore, Sargassum species in general are characterized by their high polyphenol content relative to Ulva species and Porphyra species (García-Casal et al, 2009). Polyphenols are well-known for their antioxidant properties and protect HaCaT keratinocytes against UVBinduced phototoxic stress and DNA damage (Svobodová et al, 2009;Wu et al, 2009). Recently, it has been reported that apo-9 0 -fucoxanthinone isolated from S. muticum showed anti-inflammatory effects (Chae et al, 2013;Yang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

The ethyl acetate fraction ofSargassum muticumattenuates ultraviolet B radiation-induced apoptotic cell death via regulation of MAPK- and caspase-dependent signaling pathways in human HaCaT keratinocytes

Piao

Kim

Zheng

et al. 2014

Pharmaceutical Biology

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Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju, Republic of Korea AbstractContext: Our previous work demonstrated that an ethyl acetate extract derived from Sargassum muticum (Yendo) Fenshol (SME) protected human HaCaT keratinocytes against ultraviolet B (UVB)-induced oxidative stress by increasing antioxidant activity in the cells, thereby inhibiting apoptosis. Objective: The aim of the current study was to further elucidate the anti-apoptotic mechanism of SME against UVB-induced cell damage. Materials and methods: The expression levels of several apoptotic-associated and mitogenactivated kinase (MAPK) signaling proteins were determined by western blot analysis of UVB-irradiated HaCaT cells with or without prior SME treatment. In addition, the loss of mitochondrial membrane potential (D m ) was detected using flow cytometry or confocal microscopy and the mitochondria membrane-permeate dye, JC-1. Apoptosis was assessed by quantifying DNA fragmentation and apoptotic body formation. Furthermore, cell viability was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Results: SME absorbed electromagnetic radiation in the UVB range (280-320 nm) of the UV/visible light spectrum. SME also increased Bcl-2 and Mcl-1 expression in UVB-irradiated cells and decreased the Bax expression. Moreover, SME inhibited the UVB-induced disruption of mitochondrial membrane potential and prevented UVB-mediated increases in activated caspase-9 and caspase-3 (an apoptotic initiator and executor, respectively) levels. Notably, treatment with a pan-caspase inhibitor enhanced the anti-apoptotic effects of SME in UVBirradiated cells. Finally, SME reduced the UVB-mediated phosphorylation of p38 MAPK and JNK, and prevented the UVB-mediated dephosphorylation of Erk1/2 and Akt. Discussion and conclusion: The present results indicate that SME safeguards HaCaT keratinocytes from UVB-mediated apoptosis by inhibiting a caspase-dependent signaling pathway.

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Lonicera caerulea and Vaccinium myrtillus fruit polyphenols protect HaCaT keratinocytes against UVB-induced phototoxic stress and DNA damage

Cited by 65 publications

References 43 publications

Edible flowers — antioxidant activity and impact on cell viability

Edible flowers — antioxidant activity and impact on cell viability

Antocianinų kiekis sausmedžio (Lonicera kamtschatica) klonuose šaldymo metu, priklausomai nuo antioksidantinės ir maistinės vertės rodiklių

The ethyl acetate fraction ofSargassum muticumattenuates ultraviolet B radiation-induced apoptotic cell death via regulation of MAPK- and caspase-dependent signaling pathways in human HaCaT keratinocytes

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