Lonicera caerulea berry extract suppresses lipopolysaccharide-induced inflammation via Toll-like receptor and oxidative stress-associated mitogen-activated protein kinase signaling

Abstract

The protective effects of Lonicera caerulea berry extract (LCBE) against hepatic inflammation and the underlying mechanisms were investigated in a rat model of lipopolysaccharide (LPS)-induced chronic liver inflammation. Male Sprague-Dawley rats were injected with LPS (200 μg per kg bw) with or without LCBE co-administration (50, 100 and 200 mg per kg bw intragastrically once daily) for 4 weeks. We found that LCBE supplementation inhibited the increase in Toll-like receptor (TLR)2 and TLR4 expression induced by LPS, while preventing glutathione depletion and reactive oxidative species generation and abrogating increases in C-reactive protein and interleukin-6 levels, restoring alanine and aspartate aminotransferase activities, and blocking the phosphorylation of p38 and c-Jun N-terminal kinase mitogen-activated protein kinases (MAPKs). The protective effects of LCBE against liver damage caused by LPS were dose-dependent. These results demonstrate that LCBE suppresses liver inflammation caused by LPS via inhibition of TLR and MAPK signaling and oxidative stress pathways, and suggest that LCBE treatment can potentially prevent chronic liver injury.

Blueberry Malvidin-3-galactoside Suppresses Hepatocellular Carcinoma by Regulating Apoptosis, Proliferation, and Metastasis Pathways In Vivo and In Vitro

“…It is generally believed that ingestion of bioactive compounds from fruits and vegetables can benefit individuals with liver damage. , Berries are rich in bioactive compounds and have thus attracted increased research attention in recent years for their potential chemopreventive effects and therapeutic potential against cancers . Blueberry is recognized worldwide as a valuable fruit because of its abundant bioactive compounds, particularly anthocyanins.…”

Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (Lonicera caerulea L.) in the Course of Experimental Trichinellosis in Mice

“…The excretory-secretory (ES) products of helminths can inhibit activation of NF-κB that regulates LPS-stimulated pro-inflammatory response in macrophages [ 4 ]. Wang et al [ 32 ] reported that LC berry extract may also affect immune cells via inhibition of LPS-induced expression of Toll-like receptors (TLRs) TLR2 and TLR4. In the course of T. spiralis infection TLRs may regulate the response of dendritic cells (DCs) and macrophages, as well as the other immune cells of the innate immune system such as mast cells [ 33 ].…”

Effect of purified anthocyanins or anthocyanin-rich extracts on C-reactive protein levels: a systematic review and meta-analysis of randomised clinical trials

“…In addition, anthocyanins exhibit anti-inflammatory effects by suppressing the synthesis of nitric oxide and reducing the level of TNF-α, IL-1β and IL-6 (11,35) . Meanwhile, the findings of some animal (14)(15)(16)(17)(18) and epidemiological (10,19,20,37) studies showed a meaningful effect of anthocyanins on the reduction of CRP levels. However, the mechanisms through which anthocyanins decrease CRP concentrations are not fully known.…”

“…Part of these protective effects have been attributed to the anti-inflammatory effect of anthocyanins. Findings of some animal (14)(15)(16)(17)(18) and epidemiologic (10,19,20) studies have shown positive effects of anthocyanins on the reduction of CRP concentration. However, results of randomised clinical trials (RCT) regarding the effect of anthocyanins on CRP levels are contradictory.…”