Lonicera confusa has an anatomical mechanism to respond to calcium-rich environment

Wang, Geng; Li, Maoteng; Fang-xu, Zhong; Fu, Chunhua; Sun, Jing; Yu, Liang

doi:10.1007/s11104-010-0549-1

Cited by 23 publications

(17 citation statements)

References 59 publications

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“…Therefore, it is likely that calcicole species have a tightly controlled or reduced uptake of Ca to survive in calcareous soils (Jefferies and Willis 1964, Raza et al 2000, Valentinuzzi et al 2015. In addition, they may have the capacity for compartmentation and/or physiological inactivation of Ca, such as producing Ca oxalate (Fink 1991, Webb 1999 or Ca sulfate (He et al 2014, Reid et al 2016, excreting Ca salts via stomata, or storing Ca 2+ in leaf glands and trichomes (Silva et al 1996, Charisios et al 2003, Wu et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Effects of calcium and its interaction with phosphorus on the nutrient status and growth of three Lupinus species

Ding

Clode

Clements

et al. 2018

Physiologia Plantarum

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Phosphorus (P)-deficiency symptoms are known for Lupinus species grown in calcareous soil, but we do not know if this is due to a high calcium (Ca) availability or a low P availability in the soil. To address this problem, we explored both the effects of Ca and its interactions with P on nutrient status and growth of three Lupinus species. Two Ca-sensitive genotypes (L. angustifolius L. P26723 and L. cosentinii Guss. P27225) and two Ca-tolerant genotypes (L. angustifolius L. cv Mandelup and L. pilosus Murr. P27440) were grown hydroponically at two P (0.1 and 10 μM) and three Ca (0.1, 0.6 and 6 mM) levels. Leaf symptoms and biomass were recorded, whole leaf and root nutrient concentrations were analysed, and leaf cellular P and Ca concentrations were determined. Phosphorus-deficiency symptoms were only observed in the Ca-sensitive genotypes. Among all the genotypes in this study, the Ca-tolerant L. pilosus showed an ability to maintain stable leaf Ca and P concentrations whereas the Ca-tolerant L. angustifolius cv Mandelup did not maintain a stable whole leaf Ca concentration, but maintained a low cytosolic Ca concentration through effective Ca compartmentation. However, the two Ca-sensitive genotypes, L. angustifolius P26723 and L. cosentinii, did not exhibit an ability to maintain a stable whole leaf Ca concentration or effectively compartmentalise Ca. Therefore, having the capacity to maintain a stable whole leaf Ca concentration or effectively compartmentalising Ca in leaves are likely critical for Lupinus species to be Ca tolerant.

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Section: Introductionmentioning

confidence: 99%

Effects of calcium and its interaction with phosphorus on the nutrient status and growth of three Lupinus species

Ding

Clode

Clements

et al. 2018

Physiologia Plantarum

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“…Moreover, for Lonicera confusa, which inhabits karst areas, excess Ca can not only be deposited within plants via storage in leaf glands and trichomes, but can also be excreted via stomata (Wu et al, 2010). In this study, significant positive selection signals were detected in the Primulina Ca 2þ -permeable channel TPC1, and a few sites were identified to be under positive selection.…”

Section: Discussionmentioning

confidence: 63%

Adaptive molecular evolution of the two-pore channel 1 geneTPC1in the karst-adapted genusPrimulina(Gesneriaceae)

Tao

Feng

et al. 2016

Ann Bot

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Background and Aims Limestone karst areas possess high floral diversity and endemism. The genus Primulina, which contributes to the unique calcicole flora, has high species richness and exhibit specific soil-based habitat associations that are mainly distributed on calcareous karst soils. The adaptive molecular evolutionary mechanism of the genus to karst calcium-rich environments is still not well understood. The Ca 2þ -permeable channel TPC1 was used in this study to test whether its gene is involved in the local adaptation of Primulina to karst high-calcium soil environments.Methods Specific amplification and sequencing primers were designed and used to amplify the full-length coding sequences of TPC1 from cDNA of 76 Primulina species. The sequence alignment without recombination and the corresponding reconstructed phylogeny tree were used in molecular evolutionary analyses at the nucleic acid level and amino acid level, respectively. Finally, the identified sites under positive selection were labelled on the predicted secondary structure of TPC1.Key Results Seventy-six full-length coding sequences of Primulina TPC1 were obtained. The length of the sequences varied between 2220 and 2286 bp and the insertion/deletion was located at the 5 0 end of the sequences. No signal of substitution saturation was detected in the sequences, while significant recombination breakpoints were detected. The molecular evolutionary analyses showed that TPC1 was dominated by purifying selection and the selective pressures were not significantly different among species lineages. However, significant signals of positive selection were detected at both TPC1 codon level and amino acid level, and five sites under positive selective pressure were identified by at least three different methods.Conclusions The Ca 2þ -permeable channel TPC1 may be involved in the local adaptation of Primulina to karst Ca 2þ -rich environments. Different species lineages suffered similar selective pressure associated with calcium in karst environments, and episodic diversifying selection at a few sites may play a major role in the molecular evolution of Primulina TPC1.

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“…We found that Ca-tolerant Lupinus species tended to have tight control over Ca uptake and/or translocation from roots to leaves, or better Ca compartmentation at the cellular level (Ding et al 2018a). These abilities likely play an important role in the tolerance of some Lupinus species to calcareous soils, as suggested for other calcicole species (He et al 2014;Jefferies and Willis 1964;Raza et al 2000;Valentinuzzi et al 2015;Webb 1999;Wu et al 2011).…”

Section: Introductionmentioning

confidence: 75%

Is pH the key reason why some Lupinus species are sensitive to calcareous soil?

2018

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Aims: Previous studies have shown that pH, rather than calcium (Ca), is the main reason why some Lupinus species are sensitive to nutrient solutions mimicking calcareous soils; however, a hydroponic system is quite different from soil systems, and plants may respond differently to these two growing conditions. Thus, studies with Lupinus species grown in calcareous soils are needed. Methods: Two calcicole and two calcifuge species were grown in river sand with different Ca forms and amounts, pH levels, and [bicarbonate (HCO3-)] (which is produced by calcium carbonate (CaCO3)). Leaf symptoms, leaf area, gas exchange, biomass, and root morphology were recorded; whole leaf and root nutrient concentrations were analysed. Results: We observed leaf chlorosis of the youngest leaves under high pH (adjusted by KOH) and high pH + high Ca (representing high [HCO3-], high pH and high Ca) treatments for all Lupinus species. However, after two weeks, leaf chlorosis of all Lupinus species under high pH started to disappear, with calcicole species fully, and calcifuge species only partly recovering. Leaf chlorosis symptoms of calcicole species under high pH + high Ca partly disappeared as well, while those of calcifuge species did not disappear at all. Conclusions: High pH (resulting from either KOH or HCO3-) inhibited root growth, and subsequently uptake of some nutrients and shoot growth of Lupinus species. However, the strong buffering capacity of HCO3is the key factor determining if Lupinus species can survive in calcareous soils. Among all studied Lupinus species, L. pilosus was the most tolerant to high [HCO3-] and/or high pH, followed by L. cosentinii and L. angustifolius, while L. hispanicus was the most sensitive.

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Lonicera confusa has an anatomical mechanism to respond to calcium-rich environment

Cited by 23 publications

References 59 publications

Effects of calcium and its interaction with phosphorus on the nutrient status and growth of three Lupinus species

Effects of calcium and its interaction with phosphorus on the nutrient status and growth of three Lupinus species

Adaptive molecular evolution of the two-pore channel 1 geneTPC1in the karst-adapted genusPrimulina(Gesneriaceae)

Is pH the key reason why some Lupinus species are sensitive to calcareous soil?

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