Looking for Sensitization Profiles In Different Populations by Recombinant Allergens

Asero, Riccardo

doi:10.1159/000363448

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…In conclusion, this study confirms the high clinical relevance of studying large cohorts of patients by recombinant allergens [17,28], and shows that profilin sensitizes even preschool children [13,27] and can contribute significantly to the development of food allergies due to its ubiquitous distribution in the vegetable kingdom. Given the epidemiological relevance of sensitization to profilin in childhood, the hypothesis that an early administration of pollen-specific immunotherapy may prevent sensitization to this and other annoying panallergens deserves to be investigated.…”

Section: Discussionsupporting

confidence: 81%

Prevalence and Clinical Relevance of IgE Sensitization to Profilin in Childhood: A Multicenter Study

Asero

Tripodi

Dondi

et al. 2015

Int Arch Allergy Immunol

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Background: Little is known about the prevalence and clinical relevance of hypersensitivity to the plant panallergen profilin in children. Objectives: The present study aimed to investigate prevalence, risk factors and clinical relevance of profilin sensitization in a large cohort of Italian children of different ages living in different geographic areas. Methods: Children with pollen allergy enrolled by 16 pediatric outpatient clinics sited in three main geographic areas of Italy were studied. SPT were carried out with commercial pollen extracts and a commercial purified date palm pollen profilin. IgE specific for allergenic pollen molecules, Phl p 12 (grass profilin) and Pru p 3 (peach lipid transfer protein) were tested by ImmunoCAP FEIA. Results: IgE to Phl p 12 (≥0.35 kU/l) was observed in 296 of the 1,271 participants (23%), including 17 of the 108 (16%) preschool children. Profilin SPT was positive (≥3 mm) in 320/1,271 (25%) participants. The two diagnostic methods were concordant in 1,151 (91%, p < 0.0001) cases. Phl p 12 IgE prevalence declined from northern to southern Italy and was directly associated with IgE to Phl p 1 and/or Phl p 5 and Ole e 1. Among children with IgE to Phl p 12, OAS was provoked by kiwi, melon, watermelon, banana, apricot and cucumber. Conclusions: Profilin sensitization is very frequent among pollen-allergic children, occurs at a very young age and contributes to the development of childhood OAS with a typical pattern of offending foods. Pediatricians should always consider IgE sensitization to profilin while examining pollen-allergic children, even if they are at preschool age.

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Section: Discussionsupporting

confidence: 81%

Prevalence and Clinical Relevance of IgE Sensitization to Profilin in Childhood: A Multicenter Study

Asero

Tripodi

Dondi

et al. 2015

Int Arch Allergy Immunol

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“…Also, it can be expected that CDR will improve in vivo diagnostics in the future - scientists expect the SPT to be replaced with CDR ( 60 ). Besides, as the CRD enables identification of genuine and cross-reactive components, this method will help in the individualized approach of ASIT, since ASIT should be applied if the patient demonstrates hypersensitivity to genuine allergens ( 5 , 61 , 62 ).…”

Section: Benefits Of the Use Of Crdmentioning

confidence: 99%

The potential of component-resolved diagnosis in laboratory diagnostics of allergy

Dodig¹,

Čepelak

2018

Biochemia Medica

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The initial laboratory approach in the diagnosis of allergies is to detect the type of allergic reaction, i.e. whether the patient’s allergy is mediated by immunoglobulin E (IgE) or not. For this purpose, the concentration of total serum IgE (tIgE) and specific IgE (sIgE) are determined. Progress in laboratory diagnostics is the use of component-resolved diagnosis (CRD) which implies determination of sIgE against purified native and recombinant allergenic molecules. Component-resolved diagnosis is used in laboratory practice as singleplex and multiplex assays. The choice of allergen for singleplex assay is based on anamnesis, clinical findings of a patient and on skin prick test results. Multiplex-microarray assays simultaneously determine multiple sIgE’s against numerous allergens. The goal of CRD is to distinguish the true allergens from the cross-reactive allergen molecules. Component-resolved diagnosis allows predicting the risk of severe symptoms, as well as anticipating the development of allergies. Thus, determination of sIgE against allergenic components may significantly improve current diagnostics of allergy. Since this method is applied in laboratory practice just a few years, it is necessary to acquire new knowledge and experience, to establish good co-operation between specialist in medical biochemistry and laboratory medicine and the specialist allergologist, so that the method can be applied in a rational manner. Component-resolved diagnosis will significantly improve the diagnostics of IgE-mediated allergy in the future. The aim of this article is to present potentials of CRD in the laboratory diagnostics of allergy mediated by IgE.

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“…Molecule-based allergy diagnosis uses purified natural and recombinant allergens to elucidate the sensitization patterns of a patient at the molecular level [38]. This procedure can (i) increase the diagnostic accuracy, (ii) distinguish genuine sensitization from sensitization due to cross-reactivity, (iii) help to assess the risk and type of allergic reaction, and (iv) facilitate the selection of eligible patients and suitable allergens for allergen-specific immunotherapy [39].…”

Section: Recombinant Allergens In Diagnostic Testsmentioning

confidence: 99%

Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy

Tscheppe

Breiteneder²

2017

Int Arch Allergy Immunol

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The years 1988-1995 witnessed the beginning of allergen cloning and the generation of recombinant allergens, which opened up new avenues for the diagnosis and research of human allergic diseases. Most crystal and solution structures of allergens have been obtained using recombinant allergens. Structural information on allergens allows insights into their evolutionary biology, illustrates clinically observed cross-reactivities, and makes the design of hypoallergenic derivatives for allergy vaccines possible. Recombinant allergens are widely used in molecule-based allergy diagnosis such as protein microarrays or suspension arrays. Recombinant technologies have been used to produce well-characterized, noncontaminated vaccine components with known biological activities including a variety of allergen derivatives with reduced IgE reactivity. Such recombinant hypoallergens as well as wild-type recombinant allergens have been used successfully in several immunotherapy trials for more than a decade to treat birch and grass pollen allergy. As a more recent application, the development of antibody repertoires directed against conformational epitopes during immunotherapy has been monitored by recombinant allergen chimeras. Although much progress has been made, the number and quality of recombinant allergens will undoubtedly increase and keep improving our knowledge in basic scientific investigations, diagnosis, and therapy of human allergic diseases.

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Looking for Sensitization Profiles In Different Populations by Recombinant Allergens

Cited by 4 publications

References 9 publications

Prevalence and Clinical Relevance of IgE Sensitization to Profilin in Childhood: A Multicenter Study

Prevalence and Clinical Relevance of IgE Sensitization to Profilin in Childhood: A Multicenter Study

The potential of component-resolved diagnosis in laboratory diagnostics of allergy

Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy

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