Looking inside phytoplasma-infected sieve elements: A combined microscopy approach using Arabidopsis thaliana as a model plant

Pagliari, Laura; Martini, Marta; Loschi, Alberto; Musetti, Rita

doi:10.1016/j.micron.2016.07.007

Cited by 21 publications

(29 citation statements)

References 64 publications

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“…P. asteris’, 16SrI-B subgroup), called Chrysanthemum yellows (CY) phytoplasma ( Lee et al , 2004 ). As extensively described by Pagliari et al (2016) , the fourth and fifth instars of the insect vector Euscelidius variegatus ( Bosco et al , 1997 , 2007 ) were transferred to CY-infected daisy plants ( Chrysanthemum carinatum Schousboe), used as the source of inoculum, for a 7 d acquisition-feeding period. Thirty days after nymph transfer, 45-day-old A. thaliana plants were individually exposed to three infective insects.…”

Section: Methodsmentioning

confidence: 99%

“…Although their impact on the host is of major interest for agricultural yield and basic sciences, virtually nothing is known about the basics of the interaction between phytoplasmas and SEs. Phytoplasmas greatly affect the ultrastructural organization of SEs, probably by establishing connections to acquire host-derived nutrients ( Buxa et al , 2015 ; Musetti et al , 2016 ; Pagliari et al , 2016 ). However, it is unclear which of the ultrastructural modifications in SEs are related to resource acquisition by phytoplasmas and which are to be regarded as protective measures of the host plant against phytoplasma spread ( Musetti et al , 2016 ; Pagliari et al , 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…Phytoplasmas greatly affect the ultrastructural organization of SEs, probably by establishing connections to acquire host-derived nutrients ( Buxa et al , 2015 ; Musetti et al , 2016 ; Pagliari et al , 2016 ). However, it is unclear which of the ultrastructural modifications in SEs are related to resource acquisition by phytoplasmas and which are to be regarded as protective measures of the host plant against phytoplasma spread ( Musetti et al , 2016 ; Pagliari et al , 2016 ). In infected Vicia faba plants, phytoplasmas also trigger structural modifications of forisomes in SEs ( Musetti et al , 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Filamentous sieve element proteins are able to limit phloem mass flow, but not phytoplasma spread

Pagliari

Buoso

Santi

et al. 2017

Journal of Experimental Botany

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Filamentous sieve element proteins are able to limit phloem mass flow, but not phytoplasma spread

Pagliari

Buoso

Santi

et al. 2017

Journal of Experimental Botany

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“…Despite the fact that microbial effectors recognized by the PRR machinery are conserved 31 , nothing is known about phytoplasma effectors that are able to trigger RIN4-correlated defense response or, more specifically, about the role of RIN4 in Arabidopsisphytoplasma interaction. Since Arabidopsis is not a natural host of CY phytoplasma, but is used only for its convenience as a model plant 32,33 , Arabidopsis may not have developed any recognition system via AtRIN4 against CY-phytoplasma effectors in the course of evolution.…”

Section: Free Atseor2 Enhances the Expression Of Several Defence Genesmentioning

confidence: 99%

What Slows Down Phytoplasma Proliferation? Speculations on the Involvement of AtSEOR2 Protein in Plant Defence Signalling

Pagliari

Buoso

Santi

et al. 2018

Plant Signaling & Behavior

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Considering the crude methods used to control phytoplasma diseases, a deeper knowledge on the defence mechanisms recruited by the plant to face phytoplasma invasion is required. Recently, we demonstrated that Arabidopsis mutants lacking AtSEOR1 gene showed a low phytoplasma titre. In wild type plants AtSEOR1 and AtSEOR2 are tied in filamentous proteins. Knockout of the AtSEOR1 gene may pave the way for an involvement of free AtSEOR2 proteins in defence mechanisms. Among the proteins conferring resistance against pathogenic bacteria, AtRPM1-interacting protein has been found to interact with AtSEOR2 in a high-quality, matrix-based yeast-two hybrid assay. For this reason, we investigated the expression levels of Arabidopsis AtRIN4, and the associated AtRPM1 and AtRPS2 genes in healthy and Chrysanthemum yellows-infected wild-type and Atseor1ko lines.

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“…White leaf disease in sugarcane is caused by phytoplasma parasites that destroy the plant's chlorophyll, which is responsible for photosynthesis and the green color of the leaves. Phytoplasma are bacterial parasites classified as prokaryotes of the mollicutes class (Pagliari et al, 2016). They have no cell wall and are of an uncertain shape of 400-900 nm.…”

Section: Introductionmentioning

confidence: 99%

Treating White Leaf Disease in Sugarcane Cuttings Using Solution Plasma

Sroykaew¹,

Leksakul²,

Boonyawan³

et al. 2018

CMUJNS

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Sugarcane, an important agricultural crop, is susceptible to white leaf disease, which can severely damage the crop and income. The disease is caused by phytoplasma, a prokaryote in the class mollicutes. This study investigated the efficacy of treating the disease by sterilization with solution plasma technology, including wire-to-wire, plasma jet, and gliding arc systems. Nested PCR and a scanning electron microscope were used to examine the phytoplasma disease and evaluate the effectiveness of the various solution plasma treatments. The most effective treatments, in terms of balancing plant growth and disease reduction, were the wire-to-wire system, with infected specimens immersed during discharge for 2 min with discharge repeated every 2 min, for 120 min (Exp 13); and the plasma jet system, with infected specimens immersed during discharge for 30 min and then continuously immersed for 180 min (Exp 18). The wire-to-wire treatment yielded 50% disease-free sugarcane specimens with 80% growth; the plasma jet system yielded fewer disease-free sugarcane specimens (40%), but more growth (90%). Additionally, SEM results showed that the solution plasma treatments as a group reduced disease incidence by about one third compared to the controls.

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Looking inside phytoplasma-infected sieve elements: A combined microscopy approach using Arabidopsis thaliana as a model plant

Cited by 21 publications

References 64 publications

Filamentous sieve element proteins are able to limit phloem mass flow, but not phytoplasma spread

Filamentous sieve element proteins are able to limit phloem mass flow, but not phytoplasma spread

What Slows Down Phytoplasma Proliferation? Speculations on the Involvement of AtSEOR2 Protein in Plant Defence Signalling

Treating White Leaf Disease in Sugarcane Cuttings Using Solution Plasma

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