Loop mediated isothermal amplification for detection of foodborne parasites: A journey from lab to lab-on-a-chip

Toward waterborne protozoa detection using sensing technologies

“…Nevertheless, serological methods may provide cross reaction, and are not suitable for screening a population at the time of outbreaks or most intestinal FWP. In addition, due to expensive equipment and instruments for molecular methods, and the need for a well-equipped laboratory, most molecular-based approaches are not suitable for investigation of an outbreak (Mohammad Rahimi et al, 2019;Mahdavi Abhari et al, 2023). Therefore, focus has been dramatically increased on biosensors, such as POC tests, which can provide enough sensitivity and specificity, without the need for a well-trained technician or a specific facility.…”

“…In addition, a microfluidic device based on the detection of C. parvum and G. lamblia oocysts/cysts using positive pressure was developed that identified C. parvum and G. lamblia fluorescent labels, while the staining solution was 10 to 100 times more diluted than the recommended concentration in the conventional glass method (Zhu et al, 2004). Microfluidic trapping devices can also be integrated with on-chip molecular methods for further applications (Mahdavi Abhari et al, 2023). Molecular sensing techniques include pre-amplification of the microorganism genomic material, either via fluorescence or electrochemical tools.…”