Loop‐mediated isothermal amplification (LAMP): An effective molecular point‐of‐care technique for the rapid diagnosis of coronavirus SARS‐CoV‐2

Chaouch, Melek

doi:10.1002/rmv.2215

Cited by 116 publications

(74 citation statements)

References 77 publications

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“…However, these limitations should be weighed against the many advantages. LAMP is a molecular assay that is growing in use as a cost-effective tool for SARS-CoV-2 surveillance and diagnostics [ 26 , [41] , [42] , [43] ]. Not only it is valid as a naked-eye colorimetric assay, as herein demonstrated, but it is also amenable to spectrophotometric quantification [27] .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa

Baba¹,

Bitew²,

Fokam³

et al. 2021

EClinicalMedicine

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Background Management and control of the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus SARS-CoV-2 is critically dependent on quick and reliable identification of the virus in clinical specimens. Detection of viral RNA by a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a simple, reliable and cost-effective assay, deployable in resource-limited settings (RLS). Our objective was to evaluate the intrinsic and extrinsic performances of RT-LAMP in RLS. Methods This is a multicenter prospective observational study of diagnostic accuracy, conducted from October 2020 to February 2021 in four African Countries: Cameroon, Ethiopia, Kenya and Nigeria; and in Italy. We enroled 1657 individuals who were either COVID-19 suspect cases, or asymptomatic and presented for screening. RNA extracted from pharyngeal swabs was tested in parallel by a colorimetric RT-LAMP and by a standard real time polymerase chain reaction (RT-PCR). Findings The sensitivity and specificity of index RT LAMP compared to standard RT-PCR on 1657 prospective specimens from infected individuals was determined. For a subset of 1292 specimens, which underwent exactly the same procedures in different countries, we obtained very high specificity (98%) and positive predictive value (PPV = 99%), while the sensitivity was 87%, with a negative predictive value NPV = 70%, Stratification of RT-PCR data showed superior sensitivity achieved with an RT-PCR cycle threshold (Ct) below 35 (97%), which decreased to 60% above 35. Interpretation In this field trial, RT-LAMP appears to be a reliable assay, comparable to RT-PCR, particularly with medium-high viral loads (Ct < 35). Hence, RT-LAMP can be deployed in RLS for timely management and prevention of COVID-19, without compromising the quality of output.

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Section: Discussionmentioning

confidence: 99%

Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa

Baba¹,

Bitew²,

Fokam³

et al. 2021

EClinicalMedicine

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“…LAMP tests have been proposed as an interesting option for the diagnosis of SARS-CoV-2 infection because they are rapid, sensitive and effective visual nucleic acid amplification methods [ 3 , 11 , 12 ]. Several reports have described the development of this type of assays for SARS-CoV-2 detection [ 13 , 14 , 15 ], and WHO encourages the analysis of their analytical and clinical performance, so as to demonstrate their potential operational utility and sharing of data [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, in areas with widespread transmission, one single discriminatory target might be acceptable. Additionally, other amplification methods, such as isothermal nucleic acid amplification technologies (e.g., reverse transcription loop-mediated isothermal amplification, RT-LAMP), have emerged as useful alternatives in the context of the current COVID-19 pandemic [ 3 , 4 ]. However, validation of the analytical and clinical performance of these assays is encouraged to increase access to reliable SARS-CoV-2 testing.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of RT-qPCR and Loop-Mediated Isothermal Amplification (LAMP) Assays for the Detection of SARS-CoV-2 in Argentina

Fellner

Bonaventura

Basiletti

et al. 2021

Genes

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Our aim was to evaluate the analytical and clinical performance of the SARS-CoV-2 molecular detection kits used in Argentina. Nine real-time reverse-transcription polymerase chain reaction (RT-qPCR) and three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays were evaluated using the World Health Organization (WHO) recommended test as reference method. A secondary standard calibrated for the E, N and RdRp genes against the Pan American Health Organization—World Health Organization—International Standard was used to calculate the limit of detection (LoD). A panel of artificial clinical samples, 32 positive and 30 negative for SARS-CoV-2, were analyzed to estimate the kappa concordance (κ) and the diagnostic performance. Differences among the LoD values for the target genes amplified by each kit were >1 log copies/reaction. The κ for the RT-qPCR kits was greater than 0.9, whereas that for the RT-LAMP assays ranged from 0.75 to 0.93. The clinical performance of RT-qPCR kits showed 100% specificity and high sensitivity, although with variations according to the gene analyzed. The E and N genes provided greater clinical sensitivity, whereas the RdRp gene increased the clinical specificity. The RT-LAMP assays revealed a variable diagnostic performance. The information provided can be useful to choose the most appropriate diagnostic test and may contribute to the establishment of a consensus in the diagnosis of SARS-CoV-2 in Argentina and the region.

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“…The significant limitations of the RT-LAMP method and its use in the diagnosis of SARS-CoV-2 are not the lack of developed tests, but rather the bottlenecks that significantly limit its applicability. Of all the 32 LAMP tests described by researchers worldwide, only seven originate from Europe, and only two have been tested on biological material [131]. How many public scientific institutes have described a LAMP-based SARS-CoV-2 diagnostic kit that is already commonly used?…”

Section: Limitations Of the Lamp Methodsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

Soroкa

Wasowicz

Rymaszewska

2021

Cells

224

100

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In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.

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Loop‐mediated isothermal amplification (LAMP): An effective molecular point‐of‐care technique for the rapid diagnosis of coronavirus SARS‐CoV‐2

Cited by 116 publications

References 77 publications

Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa

Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa

Evaluation of RT-qPCR and Loop-Mediated Isothermal Amplification (LAMP) Assays for the Detection of SARS-CoV-2 in Argentina

Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?

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