Loop-mediated isothermal amplification (LAMP) coupled with bioluminescence for the detection of Listeria monocytogenes at low levels on food contact surfaces

Mikš-Krajnik, Marta; Lim, Hazel Sin Yue; Zheng, Qianwang; Turner, Matthew; Yuk, Hyun‐Gyun

doi:10.1016/j.foodcont.2015.07.035

Cited by 16 publications

(5 citation statements)

References 16 publications

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“…Some different alternatives have been recently developed for this purpose [37,38], but to the best of our knowledge, this is the first study applying RPA combined with LF for food contact surface analysis. This type of methodology has been previously applied in food samples [33,39], but no prior studies have been identified reporting its application to food-contact surfaces.…”

Section: Discussionmentioning

confidence: 99%

Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces

Azinheiro

Carvalho

Prado

et al. 2020

Foods

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The continuous contamination of foods with L. monocytogenes, highlights the need for additional controls in the food industry. The verification of food processing plants is key to avoid cross-contaminations, and to assure the safety of the food products. In this study, a new methodology for the detection of L. monocytogenes on food contact surfaces was developed and evaluated. It combines Recombinase Polymerase Amplification (RPA) with the lateral flow (LF) naked-eye detection. Different approaches for the recovery of the bacteria from the surface, the enrichment step and downstream analysis by RPA-LF were tested and optimized. The results were compared with a standard culture-based technique and qPCR analysis. Sampling procedure with sponges was more efficient for the recovery of the bacteria than a regular swab. A 24 h enrichment in ONE broth was needed for the most sensitive detection of the pathogen. By RPA-LF, it was possible to detect 1.1 pg/µL of pure L. monocytogenes DNA, and the complete methodology reached a LoD50 of 4.2 CFU/cm2 and LoD95 of 18.2 CFU/cm2. These results are comparable with the culture-based methodology and qPCR. The developed approach allows for a next-day detection without complex equipment and a naked-eye visualization of the results.

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Section: Discussionmentioning

confidence: 99%

Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces

Azinheiro

Carvalho

Prado

et al. 2020

Foods

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“…In addition, Listeria monocytogenes has strong tolerance and can grow and proliferate within extreme conditions. A large number of studies have characterized Listeria monocytogenes (Mikš‐Krajnik, Lim, & Zheng, 2016; Tang et al, 2011). The solid‐state ECL biosensor established in the present study can effectively screen the presence of Listeria monocytogenes within 4 hr.…”

Section: Discussionmentioning

confidence: 99%

Ultrasensitive detection of Listeria monocytogenes using solid‐state electrochemiluminescence biosensing based on the quenching effect of ferrocene on ruthenium pyridine

Chen

et al. 2020

Journal of Food Safety

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A solid‐state electrochemiluminescence (ECL) biosensing switch system based on the specific recognition of an aptamer and the destruction of pyridine ruthenium by ferrocene was successfully developed for the detection of Listeria monocytogenes detections. The switching system consisted of two principal parts, the ECL luminescent substrate and the ECL intensity switch. The ECL luminescent substrate was fabricated by attaching an aptamer of Listeria monocytogenes modified with DNA labeled with bipyridine ruthenium complementary to one end of the aptamer, and ferrocene‐labeled DNA complementary to the other end of the aptamer, to an electrode, and so act as an intensity switch. The identification reaction consisted of the specific binding of the aptamer with Listeria monocytogenes causing the labeled ferrocene to be removed from the luminescent substrate, the structural change resulting in a significant increase in the intensity of ECL due to the decreased quenching effect of ferrocene toward the ECL luminescent substrate. Accordingly, the change in ECL intensity indirectly reflected the concentration of Listeria monocytogenes in the sample. The results demonstrated that the system produced a good linear relationship over the concentration range of 1.4 × 101–1.4 × 106 CFU/ml. The present study established that this was a feasible approach for the detection of Listeria monocytogenes in milk and pork samples.

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“…91 Interestingly, LAMP was also used to detect Listeria monocytogenes on food contacting surfaces with an impressive reported low LOD of 1 CFU/100 cm 2 . 92 Besides RCA and LAMP, other isothermal DNA amplication techniques have also been used in microuidic systems to assist bacterial cell detection or DNA detection. 88 2.2.2 Nanoparticles.…”

Section: Signal Transduction and Amplicationmentioning

confidence: 99%

Rapid and ultra-sensitive detection of foodborne pathogens by using miniaturized microfluidic devices: a review

2016

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Loop-mediated isothermal amplification (LAMP) coupled with bioluminescence for the detection of Listeria monocytogenes at low levels on food contact surfaces

Cited by 16 publications

References 16 publications

Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces

Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces

Ultrasensitive detection of Listeria monocytogenes using solid‐state electrochemiluminescence biosensing based on the quenching effect of ferrocene on ruthenium pyridine

Rapid and ultra-sensitive detection of foodborne pathogens by using miniaturized microfluidic devices: a review

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