Lornoxicam quantification in rabbit plasma by reverse phase HPLC: Optimization and application to pharmacokinetic study

Abstract

The aim of present study was to develop a simple, rapid, selective, sensitive and robust reverse phase high‐performance liquid chromatography method for quantification of Lornoxicam and internal standard meloxicam in rabbit plasma at the wavelength of 370 nm. Protein was precipitated from rabbit plasma sample by addition of acetonitrile as a vehicle. An isocratic elution of samples was performed on capcell pak C8 column with the mobile phase consisting 5 mM phosphate buffer (pH 4.8): methanol (40:60 v/v) delivered at flow rate 1.0 mL min−1. The method was quantitatively evaluated in terms of System suitability, linearity, precision, recovery, selectivity, robustness and stability studies. The validated reverse phase high‐performance liquid chromatography method was successfully applied for the bioavailability studies of Lornoxicam. The pharmacokinetic parameters were calculated for all the investigated drugs in rabbit plasma after single‐dose administrations of pure drug and formulation of Lornoxicam. Thus, developed method is simple, convenient and suitable for the analysis of Lornoxicam in bulk drug and pharmaceutical formulations.

A Validated Simple, Rapid and Accurate RP-HPLC Approach for Measuring Vardenafil Hydrochloride Trihydrate in Bulk Drugs and Medicinal Formulations

“…The comprehensive evaluation of system suitability parameters is presented in Table 1, showcasing the robustness and reliability of the developed analytical system. [40][41][42][43][44][45] The remarkably low values of percent RSD for these parameters serve as compelling evidence of the system's exceptional reliability and consistency. Specifically, the mean peak area of 330575±0.4622, indicates excellent reproducibility, underscoring the system's ability to consistently measure the analyte's response.…”

Meloxicam quantification in rabbit plasma by RP-HPLC: optimization and application to pharmacokinetic study

“…Subsequently, rabbit blood plasma of respective groups was tested in HPLC until 48 h for the pharmacokinetic approach. Experimental pharmacokinetic parameters, test/reference ratio (T/R), confidence intervals (90 CIs), and their significant statistical numbers for MLC following oral administration of 15 mg of MELFLAM tablets ranged from 80 to 125% in accordance with the FDA Bioequivalence Guideline for AUC o-t , AUC 0-∞ , and C max [39,40].…”

Thiosemicarbazide functionalized carbon quantum dots as a fluorescent probe for the determination of some oxicams: application to dosage forms and biological fluids

“…It differs from other oxicams in that, it has a short elimination half-life (3 to 5 hours), which can be benecial in terms of tolerability. 40 Variable methods were reported for determination of LRX including spectrophotometry, 33,41 spectrouorimetry, 35 HPLC, [42][43][44] and electrochemical methods. 45,46 The previously reported methods require expensive instrumentation, tedious and timeconsuming procedures.…”