Los brotes de legionelosis en España

Olivas, J. Blanquer

doi:10.1016/s0300-2896(15)30543-3

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…We sequenced the genomes of 156 clinical and environmental samples belonging to L. pneumophila using the Illumina MiSeq platform. These strains were isolated from clinical and environmental sources sampled during the investigation of sporadic cases in Sweden and France, as well as from outbreaks in Madrid (1996) [ 57 ] and Murcia (2001) [ 58 ], Spain. Most strains corresponded to serogroup 1, but sequence types varied (Table S1).…”

Section: Resultsmentioning

confidence: 99%

Rapid adaptations of Legionella pneumophila to the human host

et al. 2023

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Legionella pneumophila are host-adapted bacteria that infect and reproduce primarily in amoeboid protists. Using similar infection mechanisms, they infect human macrophages, and cause Legionnaires’ disease, an atypical pneumonia, and the milder Pontiac fever. We hypothesized that, despite the similarities in infection mechanisms, the hosts are different enough that there exist high-selective value mutations that would dramatically increase the fitness of Legionella inside the human host. By comparing a large number of isolates from independent infections, we identified two genes, mutated in three unrelated patients, despite the short duration of the incubation period (2–14 days). One is a gene coding for an outer membrane protein (OMP) belonging to the OmpP1/FadL family. The other is a gene coding for an EAL-domain-containing protein involved in cyclic-di-GMP regulation, which in turn modulates flagellar activity. The clinical strain, carrying the mutated EAL-domain-containing homologue, grows faster in macrophages than the wild-type strain, and thus appears to be better adapted to the human host. As human-to-human transmission is very rare, fixation of these mutations into the population and spread into the environment is unlikely. Therefore, parallel evolution – here mutations in the same genes observed in independent human infections – could point to adaptations to the accidental human host. These results suggest that despite the ability of L. pneumophila to infect, replicate in and exit from macrophages, its human-specific adaptations are unlikely to be fixed in the population.

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Section: Resultsmentioning

confidence: 99%

Rapid adaptations of Legionella pneumophila to the human host

et al. 2023

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“…We sequenced the genomes of 166 clinical and environmental samples belonging to L. pneumophila using the Illumina MiSeq platform. These strains were isolated from clinical and environmental sources sampled during the investigation of sporadic cases in Sweden and France, as well as from outbreaks in Madrid (1996) [29] and Murcia (2001) [30], Spain. Most strains corresponded to serogroup 1, but sequence types varied ( Supplementary Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Rapid, in-patient adaptations of Legionella pneumophila to the human host

Leenheer

Moreno

Murray

et al. 2022

Preprint

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Legionella pneumophila are host-adapted bacteria that infect and reproduce primarily in amoeboid protists. Using similar infection mechanisms, they infect human macrophages, and cause Legionnaires' disease, an atypical pneumonia, and the milder Pontiac fever. We hypothesized that, despite these similarities, the hosts are different enough so that there exist high-selective value mutations that would dramatically increase the fitness of Legionella inside the human host. By comparing a large number of isolates from independent infections, we identified two genes, mutated in three unrelated patients, despite the short duration of the incubation period (2-14 days). One is a gene coding for an outer membrane protein (OMP) belonging to the OmpP1/FadL family. The clinical strain, carrying the mutated OMP homolog, grows faster in macrophages than the wild type strain, and thus appears to be better adapted to the human host. The other is a gene coding for a protein involved in cyclic-di-GMP regulation, which in turn modulates flagellar activity. As human-to-human transmission is very rare, fixation of these mutations into the population and spread into the environment is unlikely. Therefore, convergent evolution - here mutations in the same genes observed in independent human infections - could point to adaptations to the accidental human host. These results suggest that despite its ability to infect, replicate, and disperse from amoebae, L. pneumophila is not well adapted to the human host.

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A small outbreak of Legionnaires' disease in a cargo ship under repair

et al. 2001

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It was reported that two mechanics working on a cargo ship under repair in the port of Barcelona had died after having fever.An investigation was made into the possibility of any additional cases and the presence ofLegionella pneumophilain the ship they were repairing and in their hotel. The contaminated water system was treated with sodium hypochlorite.Both patients died after having been repeatedly diagnosed as having influenza. The two cases occurred among those who had been working with the pump of the ship's water system, while no cases were observed among the other workers (p=0.02). Various serogroups ofL. pneumophilawere isolated from the ship's water pump and distribution system. However, organism of serogroup 1, subgroup Pontiac (Knoxville) were identified with identical deoxyribonucleic acid (DNA) patterns in the lung tissue of one patient and in the cooling water circuit valve of the ship's water pump. The first postintervention control water samples showed no further growth of legionella, but serogrups 4 and 8 were identified 8 months later.This legionellosis outbreak, although small, was highly lethal, probably due to the high levels of bacteria to which the patients were exposed and also because of the failure of correct diagnosis. International recommendations on prevention and control of legionellosis, which include ships under repair, are required.

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Los brotes de legionelosis en España

Cited by 3 publications

References 28 publications

Rapid adaptations of Legionella pneumophila to the human host

Rapid adaptations of Legionella pneumophila to the human host

Rapid, in-patient adaptations of Legionella pneumophila to the human host

A small outbreak of Legionnaires' disease in a cargo ship under repair

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