The Valsequillo paleontological area in Puebla has been widely explored and several fossil localities are studied with the presence of megafaunal and micromammal remains. It has been suggested the possible presence of humans by indirect evidence from Pleistocene outcrops. Despite being a site of great paleontological interest, the geology of the area is complex and poorly defined, mainly at a local scale. We present the new Late Pleistocene locality Las Tazas, Valsequillo, Puebla, Mexico, which is located in Barranca Caulapan, bearing megafaunal remains and fossil pollen material. Twenty-two megafaunal elements and 32 fossil pollen samples were recovered from the same stratigraphic layer. The collected fossil material was deposited in the Paleontological Collection of the Benemérita Universidad Autónoma de Puebla. The fossil mammal remains represent the orders Cingulata, Perissodactyla, Artiodactyla, and Proboscidea; the families Pampatheriidae, Equidae, Bovidae, Camelidae, and Elephantidae; the genera Pampatherium, Equus, Bison, Camelops, and Mammuthus; and the species Pampatherium mexicanum, Equus conversidens, Camelops hesternus, and Mammuthus columbi. The fossil pollen of the genera Pinus and Quercus is identified; as well as pollen related to grasses (e.g., Poaceae, Asteraceae, and Amaranthaceae). The presence of Bison allows us to infer a Late Pleistocene-Early Holocene age for the fossil association. Las Tazas is the fossil locality with the greatest paleodiversity in the state of Puebla. Palynological analyses indicate a paleoenvironment of grasslands with Pinus and Quercus open forests and the presence of water bodies.