Loss and Gain of Elicitor Function of
            <i>Soybean Mosaic Virus</i>
            G7 Provoking
            <i>Rsv1</i>
            -Mediated Lethal Systemic Hypersensitive Response Maps to P3

Hajimorad, M. R.; Eggenberger, Alan L.; Hill, John H.

doi:10.1128/jvi.79.2.1215-1222.2005

Cited by 81 publications

(48 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The precise biochemical function of SMV P3, and indeed the potyviral P3 protein in general, is largely uncharacterized due to the lack of structural or functional motifs in its sequence. Roles in viral replication, movement, virulence activity, and in one case, avirulence, have been suggested (Rodriguez-Cerezo et al, 1993;Sáenz et al, 2000;Desbiez et al, 2003;Jenner et al, 2003;Choi et al, 2005;Hajimorad et al, 2005Hajimorad et al, , 2006Hjulsager et al, 2006;Eggenberger et al, 2008;). Some potyviral P3 proteins were detected in the nucleoli of early-infected cells, whereas others were detected exclusively in the cylindrical inclusions of infected cells (Rodriguez-Cerezo et al, 1993;Langenberg and Zhang, 1997).…”

mentioning

confidence: 99%

The Potyviral P3 Protein Targets Eukaryotic Elongation Factor 1A to Promote the Unfolded Protein Response and Viral Pathogenesis

et al. 2016

View full text Add to dashboard Cite

The biochemical function of the potyviral P3 protein is not known, although it is known to regulate virus replication, movement, and pathogenesis. We show that P3, the putative virulence determinant of soybean mosaic virus (SMV), targets a component of the translation elongation complex in soybean. Eukaryotic elongation factor 1A (eEF1A), a well-known host factor in viral pathogenesis, is essential for SMV virulence and the associated unfolded protein response (UPR). Silencing GmEF1A inhibits accumulation of SMV and another ER-associated virus in soybean. Conversely, endoplasmic reticulum (ER) stress-inducing chemicals promote SMV accumulation in wild-type, but not GmEF1A-knockdown, plants. Knockdown of genes encoding the eEF1B isoform, which is important for eEF1A function in translation elongation, has similar effects on UPR and SMV resistance, suggesting a link to translation elongation. P3 and GmEF1A promote each other's nuclear localization, similar to the nuclearcytoplasmic transport of eEF1A by the Human immunodeficiency virus 1 Nef protein. Our results suggest that P3 targets host elongation factors resulting in UPR, which in turn facilitates SMV replication and place eEF1A upstream of BiP in the ER stress response during pathogen infection.

show abstract

mentioning

confidence: 99%

The Potyviral P3 Protein Targets Eukaryotic Elongation Factor 1A to Promote the Unfolded Protein Response and Viral Pathogenesis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Interestingly, reciprocal substitution at the corresponding position to R787 in both virG7 and virG7d (i.e., Ile to Arg at position 788) did not compromise virulence of these viruses on Rsv1 soybeans (Table 1). Furthermore, a Lysto-Glu substitution at position 953 of virG7 and virG7d genomes, corresponding to codon 952 in SMV-N, did not compromise virulence of the two viruses on PI 96983 (Rsv1) (12,13). Hence, it is likely that the role of this group of mutations is secondary in nature.…”

Section: Resultsmentioning

confidence: 99%

“…The polyprotein amino acid positions encoded by the restriction sites in the SMV genome were 493, 736, 1036, and 1218, respectively. A PCR-based mutagenesis protocol (9,13,22) was used to reconstruct mutations associated with the adaptation of progeny viruses into the cloned SMV-N (pSMV-N) for assessment of their direct roles in adaptation. PrimeSTAR HS DNA polymerase (Takara Bio) was used during the synthesis of all point mutants (see Table S1 in the supplemental material for the list of all primers utilized).…”

Section: Methodsmentioning

confidence: 99%

Experimental Adaptation of an RNA Virus Mimics Natural Evolution

Hajimorad

Wen

Eggenberger

et al. 2011

J Virol

Self Cite

View full text Add to dashboard Cite

Identification of virulence determinants of viruses is of critical importance in virology.In search of such determinants, virologists traditionally utilize comparative genomics between a virulent and an avirulent virus strain and construct chimeras to map their locations. Subsequent comparison reveals sequence differences, and through analyses of site-directed mutants, key residues are identified. In the absence of a naturally occurring virulent strain, an avirulent strain can be functionally converted to a virulent variant via an experimental evolutionary approach. However, the concern remains whether experimentally evolved virulence determinants mimic those that have evolved naturally. To provide a direct comparison, we exploited a plant RNA virus, soybean mosaic virus (SMV), and its natural host, soybean. Through a serial in vivo passage experiment, the molecularly cloned genome of an avirulent SMV strain was converted to virulent variants on functionally immune soybean genotypes harboring resistance factor(s) from the complex Rsv1 locus. Several of the experimentally evolved virulence determinants were identical to those discovered through a comparative genomic approach with a naturally evolved virulent strain. Thus, our observations validate an experimental evolutionary approach to identify relevant virulence determinants of an RNA virus.

show abstract

“…Interestingly, a TNL gene family has been associated with the I locus (33). Similarly, resistance to Soybean mosaic virus mediated by the Rsv1 gene is represented by a range of phenotypes, including systemic necrosis (34). Furthermore, in N gene-mediated resistance to TMV, a local lesion phenotype typically develops; however, expression of various N gene mutants, in transgenic N. tabacum, triggers a systemic hypersensitive response upon infection with TMV (35).…”

Section: Discussionmentioning

confidence: 99%

A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner

Seo

Rojas

Lee

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

103

View full text Add to dashboard Cite

Genes involved in a viral resistance response in common bean (Phaseolus vulgaris cv. Othello) were identified by inoculating a geminivirus reporter (Bean dwarf mosaic virus expressing the green fluorescent protein), extracting RNA from tissue undergoing the defense response, and amplifying sequences with degenerate R gene primers. One such gene (a TIR-NBS-LRR gene, RT4-4) was selected for functional analysis in which transgenic Nicotiana benthamiana were generated and screened for resistance to a range of viruses. This analysis revealed that RT4-4 did not confer resistance to the reporter geminivirus; however, it did activate a resistance-related response (systemic necrosis) to seven strains of Cucumber mosaic virus (CMV) from pepper or tomato, but not to a CMV strain from common bean. Of these eight CMV strains, only the strain from common bean systemically infected common bean cv. Othello. Additional evidence that RT4-4 is a CMV R gene came from the detection of resistance response markers in CMV-challenged leaves of RT4-4 transgenic plants, and the identification of the CMV 2a gene product as the elicitor of the necrosis response. These findings indicate that RT4-4 functions across two plant families and is up-regulated in a non-virus-specific manner. This experimental approach holds promise for providing insights into the mechanisms by which plants activate resistance responses against pathogens.avirulence gene ͉ Cucumber mosaic virus ͉ geminivirus ͉ host defense response ͉ systemic necrosis

show abstract

Loss and Gain of Elicitor Function of Soybean Mosaic Virus G7 Provoking Rsv1 -Mediated Lethal Systemic Hypersensitive Response Maps to P3

Cited by 81 publications

References 71 publications

The Potyviral P3 Protein Targets Eukaryotic Elongation Factor 1A to Promote the Unfolded Protein Response and Viral Pathogenesis

The Potyviral P3 Protein Targets Eukaryotic Elongation Factor 1A to Promote the Unfolded Protein Response and Viral Pathogenesis

Experimental Adaptation of an RNA Virus Mimics Natural Evolution

A viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner

Contact Info

Product

Resources

About