Loss of catalase 2 activity affects the ascorbate metabolism in Arabidopsis upon heavy metal stress

Buzduga, I. M.; Волков, Р. А.; Панчук, І. І.

doi:10.15407/frg2020.04.306

Cited by 4 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Photorespiration is a side reaction of photosynthesis which produces hydrogen peroxide through glycolate pathway [ 33 ]. We found that 62 DEGs were significantly enriched in the KEGG pathways of glyoxalic acid and diacetic acid metabolism and peroxisome in AE8 ( Figure 4 A,B).…”

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis Points to the Biological Processes of Hybrid Incompatibility between Brassica napus and B. oleracea

Yue

Zheng

et al. 2023

Plants

View full text Add to dashboard Cite

Improving Brassica napus via introgression of the genome components from its parental species, B. oleracea and B. rapa, is an important breeding strategy. Interspecific hybridization between B. napus and B. rapa is compatible with high rate of survival ovules, while the hybridization between B. napus and B. oleracea is incompatible with the high occurrence of embryo abortion. To understand the diverse embryo fate in the two interspecific hybridizations, here, the siliques of B. napus pollinated with B. oleracea (AE) and B. rapa (NE) were employed for transcriptome sequencing at 8 and 16 days after pollination. Compared to NE and the parental line of B. napus, more specific differentially expressed genes (DEGs) (1274 and 1698) were obtained in AE and the parental line of B. napus at 8 and 16 days after pollination (DAP). These numbers were 51 and 5.8 times higher than the number of specific DEGs in NE and parental line of B. napus at 8 and 16 DAP, respectively, suggesting more complex transcriptional changes in AE. Most of DEGs in the terms of cell growth and cell wall formation exhibited down-regulated expression patterns (96(down)/131(all) in AE8, 174(down)/235(all) in AE16), while most of DEGs in the processes of photosynthesis, photorespiration, peroxisome, oxidative stress, and systemic acquired resistance exhibited up-regulated expression patterns (222(up)/304(all) in AE8, 214(up)/287(all) in AE16). This is in accordance with a high level of reactive oxygen species (ROS) in the siliques of B. napus pollinated with B. oleracea. Our data suggest that the disorder of plant hormone metabolism, retardation of cell morphogenesis, and the accumulation of ROS may be associated with hybrid incompatibility between B. napus and B. oleracea.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis Points to the Biological Processes of Hybrid Incompatibility between Brassica napus and B. oleracea

Yue

Zheng

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Photorespiration is a side reaction of photosynthesis, which produces hydrogen peroxide through glycolate pathway (Buzduga et al 2020). We found that 62 DEGs were signi cantly enriched in the KEGG pathways of glyoxalic acid and diacetic acid metabolism, and peroxisome in AE8 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis reveals the biological processes of hybridization incompatibility between Brassica napus and B. oleracea

Yue

Zheng

et al. 2022

Preprint

View full text Add to dashboard Cite

It is an important breeding strategy to improve Brassica napus by crossing with its parental species, B. oleracea and B. rapa. Interspecific hybridization between B. napus and B. rapa is compatible with high rate of survival embryo, while the hybridization between B. napus and B. oleracea is incompatible with high occurrence of embryo abortion. To understand the diverse embryo fate in the two interspecific hybridizations, here the siliques of B. napus pollinated with B. oleracea (AE) and B. rapa (NE) were employed for transcriptome sequencing at 8 and 16 days after pollination. Compared with NE and female, there were 1274 and 1698 differentially expressed genes (DEGs) in AE at 8 and 16 days after pollination (AE8 and AE16), which were significantly enriched into 67 and 72 GO terms, respectively. Of which 9 and 15 GO terms in cell growth, cell wall formation harbored high ratio of down-regulated expression DEGs (96/131 in AE8, 174/235 in AE16), while 12 and 17 GO terms in the processes of photosynthesis, photorespiration, peroxisome, oxidative stress and systemic acquired resistance harbored high ratio of up-regulated expression DEGs (222/304 in AE8, 214/287 in AE16). A high level of O2−, H2O2 content and antioxidation activity (SOD, CAT) was tested in the siliques of B. napus pollinated with B. oleracea. Our data suggest that disorder of plant hormone metabolism, retardation of cell morphogenesis and the accumulation of reactive oxygen species (ROS) may associate with hybridization incompatibility between B. napus and B. oleracea.

show abstract

Rapid Accumulation of Cadmium and Antioxidative Response in Tobacco Leaves

Buzduga¹,

Саламон²,

Волков³

et al. 2022

TOASJ

View full text Add to dashboard Cite

Background: Cadmium (Cd) is one of the most dangerous environmental pollutants. Plant damage caused by oxidative stress during long-term Cd accumulation is well documented, while the primary response to Cd uptake is poorly understood. Objective: We assess the short-term injury and the primary reaction of the antioxidant system to the rapid accumulation of Cd in tobacco leaves. Methods: Leaf rosettes of the 5-week-old plants without roots were exposed to 100, 500 and 5000 μM Cd chloride for 2 and 12 hours. Cd accumulation, oxidation levels of proteins and lipids, content of reduced (Asc) and oxidized (DHA) ascorbate and activity of CAT, APX and POD were determined. Results: An accumulation of Cd in high concentration, but only a relatively small increase in the oxidation of proteins and lipids was found in the leaves after 2 hours of treatment. These effects were transient and disappeared after 12 hours. No visible damage to plants was observed. After 12 hours, the total ascorbate content (Asc + DHA) decreased by 18%, remained unchanged or increased by 85% after the application of 100, 500 and 5000 µM Cd chloride. The increase in the ascorbate pool, which should be considered as a component of the protective response, was caused by the accumulation of DHA. The activity of APX and POD remained unchanged, while that of CAT decreased, indicating that antioxidant enzymes activation is not involved in the primary response to Cd. Conclusions: The primary generation of ROS induced by Cd does not appear to be a deleterious manifestation of Cd toxicity, but rather a component of stress signaling that causes activation of the protective response. Uptake of Cd caused severe damage to the plant after long-term, but not after short-term treatment, suggesting that the damage is the result of secondary effects of Cd toxicity.

show abstract

Loss of catalase 2 activity affects the ascorbate metabolism in Arabidopsis upon heavy metal stress

Cited by 4 publications

References 28 publications

Comparative Transcriptome Analysis Points to the Biological Processes of Hybrid Incompatibility between Brassica napus and B. oleracea

Comparative Transcriptome Analysis Points to the Biological Processes of Hybrid Incompatibility between Brassica napus and B. oleracea

Comparative transcriptome analysis reveals the biological processes of hybridization incompatibility between Brassica napus and B. oleracea

Rapid Accumulation of Cadmium and Antioxidative Response in Tobacco Leaves

Contact Info

Product

Resources

About