Loss of chromosome 9p is an independent prognostic factor in patients with clear cell renal cell carcinoma

Abstract

Loss of chromosome 9p has been implicated in the progression of renal cell carcinoma. We evaluated the clinical utility of fluorescence in situ hybridization analysis of loss of chromosome 9p in 73 patients with clear cell renal cell carcinomas with varied stage, size, grade, necrosis (SSIGN) scores. Loss of chromosome 9p was observed in 13 tumors (18%). The 5-year cancer-specific survival of patients without loss of chromosome 9p was 88% and was 43% in those with loss of chromosome 9p (Po0.001). Local extension of the primary tumor according to the 2002 TNM staging system, lymph node involvement, the presence of distant metastases, and the SSIGN score were the other variables that predicted cancer-specific survival in univariate analysis. Loss of chromosome 9p was an independent prognostic factor in multivariate analysis. Our data indicate that the detection of chromosome 9p loss by fluorescence in situ hybridization analysis of clear cell renal cell carcinoma adds prognostic information beyond the pathological factors included in the current predictive models for renal cell carcinoma, such as SSIGN score.

Multilocular cystic renal cell carcinoma is a subtype of clear cell renal cell carcinoma

“…The method of analysis was partially described previously. [12][13][14][15][16][17][18][19][20] In brief, for each slide, 100-150 nuclei from tumor tissue were scored for signals from probes under the fluorescence microscope with x1000 magnification. Nonneoplastic renal cortex was used as control tissue.…”

Acquired cystic disease-associated renal cell carcinoma: an immunohistochemical and fluorescence in situ hybridization study

“…This chromosomal change may refl ect the poor prognosis of ACD-associated RCC, as loss of chromosome 9p or chromosome 9 has been previously observed in clear cell RCC or sarcomatoid ACD-associated RCC. 6,[21][22][23] Finally, we conclude that the numerical abnormalities of chromosomes 3 and 16, irrespective of gain or loss, may be characteristic of ACD-associated RCC. A close relationship between ACD-associated RCC and papillary RCC may exist in some cases.…”

Renal cell carcinoma with smooth muscle stroma lacks chromosome 3p and VHL alterations

“…[4][5][6] Several other recurrent copy number changes, such as gains of 5q, have been reported in clear cell renal cell carcinomas. [7][8][9] In 2009, Shannon et al 10 described three more of these tumors and found that they were extensively positive for cytokeratin 7 by immunohistochemistry. They also performed fluorescence in situ hybridization (FISH) analysis of chromosome 3 and found loss of the entire chromosome in two tumors and loss of 3p in the third case.…”