Loss of ciliary activity in organ cultures of rat trachea treated with lipo-oligosaccharide from Haemophilus influenzae

Johnson, Alan P.; Inzana, Thomas J.

doi:10.1099/00222615-22-3-265

Cited by 74 publications

(36 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…may compromise the host defence system further; i.e. impair ciliary function, stimulate mucus production, break down local immunoglobulins and impair phagocytic function [24][25][26]. This consequently leads to an environment conducive to contiguous spread of the bacterium and a host-mediated, predominantly neutrophilic, counter effect, which results in further damage.…”

Section: The Putative Mechanisms Of Bacterial-induced Chronic Airway mentioning

confidence: 99%

Bacterial-induced release of inflammatory mediators by bronchial epithelial cells

Khair¹,

Davies²,

Devalia³

1996

Eur Respir J

165

118

View full text Add to dashboard Cite

show abstract

Section: The Putative Mechanisms Of Bacterial-induced Chronic Airway mentioning

confidence: 99%

Bacterial-induced release of inflammatory mediators by bronchial epithelial cells

Khair¹,

Davies²,

Devalia³

1996

Eur Respir J

165

118

View full text Add to dashboard Cite

show abstract

“…(LOS) [5,6] could also be having an effect. Protein D, an antigen expressed on NTHi, could also play a role in cilia slowing in our model [21,22].…”

Section: Discussionmentioning

confidence: 99%

Non-TypeableHaemophilus influenzaeDecreases Cilia Beating Via Protein Kinase C Epsilon.

Wyatt¹,

Sisson²,

DeVasure

et al. 2009

A27. Genetic and Acquired Cilia Disease

View full text Add to dashboard Cite

Background: Haemophilus influenzae infection of the nasal epithelium has long been associated with observations of decreased nasal ciliary beat frequency (CBF) and injury to the ciliated epithelium. Previously, we have reported that several agents that slow CBF also have the effect of activating protein kinase C epsilon (PKCε) activity in bronchial epithelial cells. The subsequent auto-downregulation of PKCε or the direct inhibition of PKCε leads to the specific detachment of the ciliated cells. METHODS: Primary cultures of ciliated bovine bronchial epithelial cells were exposed to filtered conditioned media supernatants from non-typeable H. influenzae (NTHi) cultures. CBF and motile points were measured and PKCε activity assayed. Results: NTHi supernatant exposure significantly and rapidly decreased CBF in a dose-dependent manner within 10 minutes of exposure. After 3 hours of exposure, the number of motile ciliated cells significantly decreased. Direct measurement of PKCε activity revealed a dose-dependent activation of PKCε in response to NTHi supernatant exposure. Both CBF and PKCε activity changes were only observed in fresh NTHi culture supernatant and not observed in exposures to heat-inactivated or frozen supernatants. Conclusions: Our results suggest that CBF slowing observed in response to NTHi is consistent with the stimulated activation of PKCε. Ciliated cell detachment is associated with PKCε autodownregulation.

show abstract

“…The lipid A portion, found as a heat-stable soluble product, inhibits the movement of cilia in rats (38,98). LOS also induces an inflammatory response from the host (226).…”

Section: Lipooligosaccharidementioning

confidence: 99%

“…Cellular damage in the host may occur as a result of numerous factors unrelated to the presence of bacteria. However, the presence of NTHi can cause stasis and loss of cilia, as well as damage and sloughing of epithelial cells due to the secretion of heat-stable substances such as LOS (38,98). LOS occurs in both cell-bound and secreted forms (76).…”

Section: Interaction With Mucinmentioning

confidence: 99%

Lower Respiratory Tract Infections

Oakes¹

2003

The Infectious Diseases Manual

View full text Add to dashboard Cite

Loss of ciliary activity in organ cultures of rat trachea treated with lipo-oligosaccharide from Haemophilus influenzae

Cited by 74 publications

References 6 publications

Bacterial-induced release of inflammatory mediators by bronchial epithelial cells

Bacterial-induced release of inflammatory mediators by bronchial epithelial cells

Non-TypeableHaemophilus influenzaeDecreases Cilia Beating Via Protein Kinase C Epsilon.

Lower Respiratory Tract Infections

Contact Info

Product

Resources

About