Loss of Circadian Behavioral Rhythms and
 per
 RNA Oscillations in the
 Drosophila
 Mutant
 timeless

Sehgal, Amita; Price, Jeffrey L.; Man, Bernice; Young, Michael W.

doi:10.1126/science.8128246

Cited by 561 publications

(409 citation statements)

References 33 publications

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“…However, we found an overt rhythm of mTim in the retina, a putative oscillatory tissue in vitro (Tosini & Menaker 1996), which was similar to the reported circadian phase and amplitude of mPer (Shearman et al 1997;Sun et al 1997)(peak/trough ratios in mPer1 and mPer2 are 2.4 and 3.5 in LD, respectively (Shearman et al 1997), and those of mTim are 3.0 in LD and 1.7 in DD). In Drosophila, the expression of timeless mRNA cycles with a large amplitude as does period, and oscillates synchronously to period (Hardin et al 1990;Sehgal et al 1994). Thus it seems that the mTim transcription in the retina is consistent with that of Drosophila, where timeless transcription is regulated by the PER/TIM complex (Dunlap 1998a,b;Schibler 1998), although the data from the SCN are not consistent with Drosophila.…”

Section: Discussionmentioning

confidence: 93%

A mammalian ortholog of Drosophila timeless, highly expressed in SCN and retina, forms a complex with mPER1

et al. 1999

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Background: It is now becoming clear that the circadian rhythm of behaviours and hormones arises from a rhythm at the level of gene expression, and that mammals and Drosophila essentially use homologous genes as molecular gears in the control of circadian oscillation. In Drosophila, the period and timeless genes form a functional unit of the clock and its autoregulatory feedback loop for circadian rhythm. However, in mammals, the counterpart of timeless has not been found.

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Section: Discussionmentioning

confidence: 93%

A mammalian ortholog of Drosophila timeless, highly expressed in SCN and retina, forms a complex with mPER1

et al. 1999

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“…Hsp90 mutant genotypes w 1118 , w*;Hsp90 e6D /TM6B-Tb 1 and Hsp90 08445 ,ry 506 /TM3-ry RK -Sb 1 -Ser 1 were obtained from the Bloomington Stock Center. y w; tim 01 , Tim 4, y w tim-luc, y w BG-luc and y per 01 w; per P -Gal4 flies have all been described previously [17,25,30,38,39].…”

Section: Materials and Methods (A) Fly Strainsmentioning

confidence: 99%

Temperature-dependent resetting of the molecular circadian oscillator inDrosophila

2014

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Circadian clocks responsible for daily time keeping in a wide range of organisms synchronize to daily temperature cycles via pathways that remain poorly understood. To address this problem from the perspective of the molecular oscillator, we monitored temperature-dependent resetting of four of its core components in the fruitfly Drosophila melanogaster: the transcripts and proteins for the clock genes period ( per) and timeless (tim). The molecular circadian cycle in adult heads exhibited parallel responses to temperature-mediated resetting at the levels of per transcript, tim transcript and TIM protein. Early phase adjustment specific to per transcript rhythms was explained by clockindependent temperature-driven transcription of per. The cold-induced expression of Drosophila per contrasts with the previously reported heatinduced regulation of mammalian Period 2. An altered and more readily reentrainable temperature-synchronized circadian oscillator that featured temperature-driven per transcript rhythms and phase-shifted TIM and PER protein rhythms was found for flies of the 'Tim 4' genotype, which lacked daily tim transcript oscillations but maintained post-transcriptional temperature entrainment of tim expression. The accelerated molecular and behavioural temperature entrainment observed for Tim 4 flies indicates that clock-controlled tim expression constrains the rate of temperature cycle-mediated circadian resetting.

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“…Three mutations called long (per l ), short (per s ) and arrhythmic (per 0 ) alter eclosion rhythms and circadian patterns of locomotor activity. A second clock gene called timeless (tim) affects circadian rhythmicity and per expression [32][33][34]. Genes per and tim are transcriptionally regulated in a cyclic manner.…”

Section: Case Studies In the Conservation Of Gene Function In Behaviourmentioning

confidence: 99%

Conservation of gene function in behaviour

Reaume

Sokolowski

2011

Phil. Trans. R. Soc. B

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Behaviour genetic research has shown that a given gene or gene pathway can influence categorically similar behaviours in different species. Questions about the conservation of gene function in behaviour are increasingly tractable. This is owing to the surge of DNA and 'omics data, bioinformatic tools, as well as advances in technologies for behavioural phenotyping. Here, we discuss how gene function, as a hierarchical biological phenomenon, can be used to examine behavioural homology across species. The question can be addressed independently using different levels of investigation including the DNA sequence, the gene's position in a genetic pathway, spatial-temporal tissue expression and neural circuitry. Selected examples from the literature are used to illustrate this point. We will also discuss how qualitative and quantitative comparisons of the behavioural phenotype, its function and the importance of environmental and social context should be used in cross-species comparisons. We conclude that (i) there are homologous behaviours, (ii) they are hard to define and (iii) neurogenetics and genomics investigations should help in this endeavour.

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Loss of Circadian Behavioral Rhythms and per RNA Oscillations in the Drosophila Mutant timeless

Cited by 561 publications

References 33 publications

A mammalian ortholog of Drosophila timeless, highly expressed in SCN and retina, forms a complex with mPER1

A mammalian ortholog of Drosophila timeless, highly expressed in SCN and retina, forms a complex with mPER1

Temperature-dependent resetting of the molecular circadian oscillator inDrosophila

Conservation of gene function in behaviour

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