Loss of CLE40, a protein functionally equivalent to the stem cell restricting signal CLV3, enhances root waving in Arabidopsis

Hobe, Martin; Müller, Ralf; Grünewald, Margit; Brand, Ulrike; Simon, Rüdiger

doi:10.1007/s00427-003-0329-5

Cited by 214 publications

(244 citation statements)

References 35 publications

Supporting

Mentioning

229

Contrasting

Unclassified

Order By: Relevance

“…Specifically, CLV3 and CLE40, which are both members of the CLE family, can function in both the root and shoot meristems (Hobe et al, 2003;Fiers et al, 2005;Ito et al, 2006). Also, WUS and WOX5, which are evolutionarily related homeodomain transcription factors, can functionally replace each other (Mayer et al, 1998;Haecker et al, 2004;Sarkar et al, 2007;Nardmann et al, 2009;Stahl et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

TheArabidopsisStem Cell Factor POLTERGEIST Is Membrane Localized and Phospholipid Stimulated

Gagne

Clark

2010

The Plant Cell

View full text Add to dashboard Cite

Stem cell maintenance and differentiation are tightly regulated in multicellular organisms. In plants, proper control of the stem cell populations is critical for extensive postembryonic organogenesis. The Arabidopsis thaliana protein phosphatase type 2C proteins POLTERGEIST (POL) and PLL1 are essential for maintenance of both the root and shoot stem cells. Specifically, POL and PLL1 are required for proper specification of key asymmetric cell divisions during stem cell initiation and maintenance. POL and PLL1 are known to be integral components of the CLE/WOX signaling pathways, but the location and mechanisms by which POL and PLL1 are regulated within these pathways are unclear. Here, we show that POL and PLL1 are dual-acylated plasma membrane proteins whose membrane localization is required for proper function. Furthermore, this localization places POL and PLL1 in proximity of the upstream plasma membrane receptors that regulate their activity. Additionally, we find that POL and PLL1 directly bind to multiple lipids and that POL is catalytically activated by phosphatidylinositol (4) phosphate [PI(4)P] in vitro. Based on these results, we propose that the upstream receptors in the CLE/WOX signaling pathways may function to either limit PI(4)P availability or antagonize PI(4)P stimulation of POL/PLL1. Significantly, the findings presented here suggest that phospholipids play an important role in promoting stem cell specification.

show abstract

Section: Introductionmentioning

confidence: 99%

TheArabidopsisStem Cell Factor POLTERGEIST Is Membrane Localized and Phospholipid Stimulated

Gagne

Clark

2010

The Plant Cell

View full text Add to dashboard Cite

show abstract

“…Due to redundancies and difficulties in identifying mutants of these small genes, studies of CLE members are challenging. Only a few CLE genes have been genetically characterized, in particular, CLV3, CLE8, CLE40, and CLE41 in Arabidopsis and FLORAL ORGAN NUMBER4 (FON4), FON2-LIKE CLE PROTEIN1 (FCP1), and FON2 SPARE1 in rice (Fletcher et al, 1999;Hobe et al, 2003;Chu et al, 2006;Suzaki et al, 2008Suzaki et al, , 2009Etchells and Turner, 2010;Fiume and Fletcher, 2012), while functions of other CLE members remain unknown.…”

mentioning

confidence: 99%

Antagonistic Peptide Technology for Functional Dissection ofCLV3/ESRGenes in Arabidopsis

et al. 2013

View full text Add to dashboard Cite

In recent years, peptide hormones have been recognized as important signal molecules in plants. Genetic characterization of such peptides is challenging since they are usually encoded by small genes. As a proof of concept, we used the wellcharacterized stem cell-restricting CLAVATA3 (CLV3) to develop an antagonistic peptide technology by transformations of wild-type Arabidopsis (Arabidopsis thaliana) with constructs carrying the full-length CLV3 with every residue in the peptidecoding region replaced, one at a time, by alanine. Analyses of transgenic plants allowed us to identify one line exhibiting a dominant-negative clv3-like phenotype, with enlarged shoot apical meristems and increased numbers of floral organs. We then performed second dimensional amino acid substitutions to replace the glycine residue individually with the other 18 possible proteinaceous amino acids. Examination of transgenic plants showed that a glycine-to-threonine substitution gave the strongest antagonistic effect in the wild type, in which over 70% of transgenic lines showed the clv3-like phenotype. Among these substitutions, a negative correlation was observed between the antagonistic effects in the wild type and the complementation efficiencies in clv3. We also demonstrated that such an antagonistic peptide technology is applicable to other CLV3/EMBRYO SURROUNDING REGION (CLE) genes, CLE8 and CLE22, as well as in vitro treatments. We believe this technology provides a powerful tool for functional dissection of widely occurring CLE genes in plants.

show abstract

“…According to this model, increased levels of CLE40 prevents WOX5 to reach the neighboring cells that ultimately lose their stem cell nature and differentiate. In cle40 and acr4 mutants, the inhibition is lost and the WOX5 expression domain is expanded resulting in the overproliferation of stem cells [56][57][58] (Figure 3). The role of plant hormones, transcription factors and microRNAs signals is well established in vasculature development.…”

Section: Clavata3/esr-related (Cle)mentioning

confidence: 99%

Signaling Peptides in Plants

Ghorbani¹

2014

Cell Dev Biol

View full text Add to dashboard Cite

In multicellular organisms, growth and development need to be precisely coordinated and are strongly relying on positional information. Positional control is achieved through exchanges of molecular messages between cells and tissues by means of cell-to-cell communication mechanisms. Especially in plants, accurate and well-controlled cell-to-cell communication networks are essential because of the complete absence of cell mobility and the presence of rigid cell walls. For many years, phytohormones were thought to be the main messengers exchanged between cells. Nevertheless, identification of systemin as the first plant signaling peptide in tomato hinted that peptide hormones were acting in plants as they were in animals. During the last decade, our knowledge of plant signaling peptides has progressed considerably and a number of signaling peptide families have been discovered and partially characterized. Here, we provide an overview of the current knowledge in signaling peptides in the model species Arabidopsis thaliana and discuss their proposed functions during plant growth and development.

show abstract

Loss of CLE40, a protein functionally equivalent to the stem cell restricting signal CLV3, enhances root waving in Arabidopsis

Cited by 214 publications

References 35 publications

TheArabidopsisStem Cell Factor POLTERGEIST Is Membrane Localized and Phospholipid Stimulated

TheArabidopsisStem Cell Factor POLTERGEIST Is Membrane Localized and Phospholipid Stimulated

Antagonistic Peptide Technology for Functional Dissection ofCLV3/ESRGenes in Arabidopsis

Signaling Peptides in Plants

Contact Info

Product

Resources

About