Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

Stecker, K.; Koschel, Annika; Wiedenmann, Bertram; Anders, Mario

doi:10.1038/sj.bjc.6605331

Cited by 14 publications

(14 citation statements)

References 19 publications

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“…Stecker et al . reported that knockdown of CXADR inhibits α-catenin expression13, but we could not obtain the same results in this study (data not shown). Thus, our results suggest that CXADR could not play a critical role in tumorigenesis.…”

Section: Discussioncontrasting

confidence: 79%

A novel monoclonal antibody targeting coxsackie virus and adenovirus receptor inhibits tumor growth in vivo

Kawada

Inoue

Kajikawa

et al. 2017

Sci Rep

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To create a new anti-tumor antibody, we conducted signal sequence trap by retrovirus-meditated expression method and identified coxsackie virus and adenovirus receptor (CXADR) as an appropriate target. We developed monoclonal antibodies against human CXADR and found that one antibody (6G10A) significantly inhibited the growth of subcutaneous as well as orthotopic xenografts of human prostate cancer cells in vivo. Furthermore, 6G10A also inhibited other cancer xenografts expressing CXADR, such as pancreatic and colorectal cancer cells. Knockdown and overexpression of CXADR confirmed the dependence of its anti-tumor activity on CXADR expression. Our studies of its action demonstrated that 6G10A exerted its anti-tumor activity primarily through both antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity. Moreover, 6G10A reacted with human tumor tissues, such as prostate, lung, and brain, each of which express CXADR. Although we need further evaluation of its reactivity and safety in human tissues, our results show that a novel anti-CXADR antibody may be a feasible candidate for cancer immunotherapy.

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Section: Discussioncontrasting

confidence: 79%

A novel monoclonal antibody targeting coxsackie virus and adenovirus receptor inhibits tumor growth in vivo

Kawada

Inoue

Kajikawa

et al. 2017

Sci Rep

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“…Since cell surface E-cadherin associates with the transcription factor β-catenin, the internalization of E-cadherin by CAR may dissociate the E-cadherin-β-catenin complex allowing for β-catenin translocation into the nucleus resulting in enhanced cell proliferation. In line with this hypothesis, knockdown of CAR has been reported to downregulate α-catenin in a human colon cancer cell line, suggesting that CAR may regulate adherens junction proteins to control cell proliferation [161]. …”

Section: Jam Family Members In Leukocyte Recruitment and Mucosal Inflmentioning

confidence: 97%

JAM-related proteins in mucosal homeostasis and inflammation

2014

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Mucosal surfaces are lined by epithelial cells that form a physical barrier protecting the body against external noxious substances and pathogens. At a molecular level, the mucosal barrier is regulated by tight junctions (TJs) that seal the paracellular space between adjacent epithelial cells. Transmembrane proteins within TJs include Junctional Adhesion Molecules (JAMs) that belong to the CTX (Cortical Thymocyte marker for Xenopus) family of proteins. JAM family encompasses three classical members (JAM-A, -B and –C) and related molecules including JAM4, JAM-Like protein (JAM-L), Coxsackie and Adenovirus Receptor (CAR), CAR-Like Membrane Protein (CLMP) and Endothelial cell-Selective Adhesion Molecule (ESAM). JAMs have multiple functions that include regulation of endothelial and epithelial paracellular permeability, leukocyte recruitment during inflammation, angiogenesis, cell migration and proliferation. In this review, we summarize the current knowledge regarding the roles of the JAM family members in the regulation of mucosal homeostasis and leukocyte trafficking with a particular emphasis on barrier function and its perturbation during pathological inflammation.

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“…The establishment of cell lines was confirmed by immunofluorescent (IF) staining, which was performed as described previously with slight modification [ 32 ]. Briefly, two samples were rinsed and fixed with precooled ethanol (10 min at −40 °C).…”

Section: Methodsmentioning

confidence: 99%

Angiostrongylus cantonensiscathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration

Long

Cao

et al. 2015

Parasite

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Although the global spread of the emerging zoonosis, human angiostrongyliasis, has attracted increasing attention, understanding of specific gene function has been impeded by the inaccessibility of genetic manipulation of the pathogen nematode causing this disease, Angiostrongylus cantonensis. Many parasitic proteases play key roles in host-parasite interactions, but those of A. cantonensis are always expressed as the inactive form in prokaryotic expression systems, thereby impeding functional studies. Hence, a lentiviral system that drives secreted expression of target genes fused to a Myc-His tag was used to obtain recombinant Ac-cathB-1 with biological activity. Although this class of proteases was always reported to function in nutrition and immune evasion in parasitic nematodes, recombinant Ac-cathB-1 was capable of hydrolysis of fibronectin and laminin as well as the extracellular matrix of IEC-6 monolayer, so that the intercellular space of the IEC-6 monolayer increased 5.15 times as compared to the control, while the shape of the adherent cells partly rounded up. This suggests a probable role for this protease in intestinal epithelial penetration. The inhibition of Ac-cathB-1 enzymatic activity with antiserum partly suppressed larval penetration ability in the isolated intestine. Thus, an effective system for heterologous expression of parasite proteases is presented for studying gene function in A. cantonensis; and Ac-cathB-1 was related to larval penetration ability in the host small intestine.

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Loss of Coxsackie and adenovirus receptor downregulates α-catenin expression

Cited by 14 publications

References 19 publications

A novel monoclonal antibody targeting coxsackie virus and adenovirus receptor inhibits tumor growth in vivo

A novel monoclonal antibody targeting coxsackie virus and adenovirus receptor inhibits tumor growth in vivo

JAM-related proteins in mucosal homeostasis and inflammation

Angiostrongylus cantonensiscathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration

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