Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells

Abstract

Abstract. Previous studies have investigated the mechanisms of immune evasion of tumor cells in numerous types of advanced solid malignant tumor, and several types of immune preparations have been administered as antitumor adjuvant therapies. However, in the majority of studies, the efficacy of therapies has been revealed to be limited. The present study aimed to investigate the immune evasion mechanisms employed by early colorectal cancer cells and the expression of the molecules associated with immune evasion during the malignant transformation process of normal colorectal epithelial cells to measure the effects of immune intervention for early colorectal cancer, and to improve the efficacy of immunotherapy. A total of 60 colorectal tissues, including 15 normal mucosa, 15 adenoma, 15 early cancer and 15 advanced cancer tissues, from patients undergoing endoscopic procedures in Huadong Hospital Affiliated to Fudan University (Shanghai, China) were collected. A comparison of baseline characteristics among these four groups was performed. The expression levels of human leukocyte antigen-A (HLA-A), apoptosis antigen 1 (Fas), c-c chemokine receptor type 5 (CCR5), Fas ligand (FasL) and HLA-E in each group were detected by immunohistochemical analysis. Furthermore, 15 patients with advanced colorectal cancer were enrolled into the present study. Advanced cancer and paracancer tissues (normal mucosal tissues 3 cm away from the margin of cancer tissues) were collected from each patient by colonoscopic biopsy. The expression levels of HLA-A, Fas, CCR5, FasL and HLA-E in each group were detected by western blot analysis. During the malignant transformation process of normal colorectal epithelial cells, the expression levels of CCR5, FasL and HLA-E increased significantly (P<0.001), whilst the expression levels of Fas reduced significantly (P=0.0271). In the early cancer group, the expression levels of Fas reduced significantly (P= 0.0239), whilst the expression levels of HLA-E increased significantly (P<0.001) compared with adenoma group. In conclusion, a loss of Fas expression and high expression levels of HLA-E may promote the immune evasion of early colorectal cancer cells.

HLA-E expression in diffuse glioma: relationship with clinicopathological features and patient survival

“…HLA-E is one of the most extensively investigated MHC class Ib molecules and plays a double role in both innate and adaptive immunity. Aberrant expression of HLA-E has been identified in various human malignancies, such as breast cancer, gastric cancer, rectal cancer, and colorectal cancer [22][23][24][25][26]. As for glioma, HLA-E has been reported to be elevated in human glioblastomas by immunohistochemistry, which was in consistent with what we demonstrated by microarray analysis in this study [19].…”

Targeting Checkpoint Receptors and Molecules for Therapeutic Modulation of Natural Killer Cells

“…As tumor cells exhibit variable expression of MHC I ligands, adoptive transfer of alloreactive NK cells has emerged as a promising strategy that overcomes this checkpoint and creates a condition of “missing-self” recognition. Some solid tumors and leukemias/lymphomas also use the upregulation of HLA-E to evade killing by NK and T cells ( 20 – 22 ). In this respect, another approach that mimics missing-self recognition is treatment with blocking antibodies against KIRs and/or NKG2A on autologous NK cells.…”

Control of NK Cell Activation by Immune Checkpoint Molecules

“…Lack of HLA-E/NKG2A interaction triggers NK cell “missing-self” response. HLA-E is frequently upregulated on cells from many hematological malignancies or solid cancers [ 144 , 145 , 146 ]. Similar to KIR blockade, anti-NKG2A antibody can remove HLA-E-mediated NK cell inhibition, tip the balance to favor stimulation through NK cell activating receptors, and augment HLA-E accessibility for CD94/NKG2C.…”