Loss of function of Arabidopsis NADP‐malic enzyme 1 results in enhanced tolerance to aluminum stress

Badia, Mariana Beatriz; Maurino, Verónica G.; Pavlovic, Tatiana; Arias, Cintia L.; Pagani, María A.; Andreo, Carlos S.; Saigo, Mariana; Drincovich, Marı́a F.; Wheeler, Mariel Claudia Gerrard

doi:10.1111/tpj.14571

Cited by 15 publications

(5 citation statements)

References 66 publications

(73 reference statements)

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“…Cellular malate synthesis and degradation are important for the regulation of the cytosolic pH (Hurth et al., 2005; Smith & Raven, 1979). The absence of NADP‐ME1 encoding ME causes an increase in the amount of malate (Badia et al., 2020). In this research, GO term and KEGG enrichment analysis showed that many genes involved in ME activity were down‐regulated in the A9N3‐treated root compared to the AA‐treated root (Figures S2 and S9; Table S1).…”

Section: Discussionmentioning

confidence: 99%

Nitrate alleviates ammonium toxicity in Brassica napus by coordinating rhizosphere and cell pH and ammonium assimilation

Li,

Yan,

Zhang

et al. 2023

The Plant Journal

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SUMMARYIn natural and agricultural situations, ammonium () is a preferred nitrogen (N) source for plants, but excessive amounts can be hazardous to them, known as toxicity. Nitrate () has long been recognized to reduce toxicity. However, little is known about Brassica napus, a major oil crop that is sensitive to high . Here, we found that can mitigate toxicity by balancing rhizosphere and intracellular pH and accelerating ammonium assimilation in B. napus. increased the uptake of and under high circumstances by triggering the expression of and transporters, while and H+ efflux from the cytoplasm to the apoplast was enhanced by promoting the expression of efflux transporters and genes encoding plasma membrane H+‐ATPase. In addition, increased pH in the cytosol, vacuole, and rhizosphere, and down‐regulated genes induced by acid stress. Root glutamine synthetase (GS) activity was elevated by under high conditions to enhance the assimilation of into amino acids, thereby reducing accumulation and translocation to shoot in rapeseed. In addition, root GS activity was highly dependent on the environmental pH. might induce metabolites involved in amino acid biosynthesis and malate metabolism in the tricarboxylic acid cycle, and inhibit phenylpropanoid metabolism to mitigate toxicity. Collectively, our results indicate that balances both rhizosphere and intracellular pH via effective transmembrane cycling, accelerates assimilation, and up‐regulates malate metabolism to mitigate toxicity in oilseed rape.

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Section: Discussionmentioning

confidence: 99%

Nitrate alleviates ammonium toxicity in Brassica napus by coordinating rhizosphere and cell pH and ammonium assimilation

Li,

Yan,

Zhang

et al. 2023

The Plant Journal

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“…In particular, NADP-ME1 is involved in the regulation of malic acid content in the root apex, and the absence of NADP-ME1 results in an increase in malic acid content. Furthermore, NADP-ME1 also affects the signal transmission process [36]. However, NADP-ME2 plays an important role in the defense mechanism of plants and seems to be involved in the production of reactive oxygen species [37].…”

Section: Iaa Mediated Amf-induced Nadp-mes Expression and Nadp-me Activitymentioning

confidence: 99%

Auxin is involved in arbuscular mycorrhizal fungi-promoted tomato growth and NADP-malic enzymes expression in continuous cropping substrates

et al. 2021

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Background Despite significant limitations of growth medium reuse, a large amount of organic substrate is reused in soilless cultivation of horticultural crops in China. Arbuscular mycorrhizal fungi (AMF) can promote nutrient absorption and improve plant tolerance to biotic and abiotic stresses. However, the mechanisms governing the effects of AMF on crop growth in organic continuous cropping substrates have not been elucidated. Results In this study, we showed that the inoculation of AMF in continuous cropping substrates promoted growth and root development, and increased the root and NADP-malic enzyme (NADP-ME) activity of tomato seedlings. Root transcriptome analysis demonstrated that the plant hormone signal transduction pathway was highly enriched, and 109 genes that positively correlated with the AMF-inoculated plant phenotype were obtained by gene set enrichment analysis (GSEA), which identified 9 genes related to indole acetic acid (IAA). Importantly, the levels of endogenous IAA in tomato seedlings significantly increased after AMF inoculation. Furthermore, the application of AMF significantly increased the expression levels of NADP-ME1 and NADP-ME2, as well as the activity of NADP-ME, and enhanced the root activity of tomato seedlings in comparison to that observed without inoculation of AMF. However, these effects were blocked in plants treated with 2,3,5-triiodobenzoic acid (TIBA), a polar transport inhibitor of IAA. Conclusions These results suggest that IAA mediates the AMF-promoted tomato growth and expression of NADP-MEs in continuous cropping substrates. The study provides convincing evidence for the reuse of continuous cropping substrates by adding AMF as an amendment.

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“…The inhibition of the root length was an initial detection of Al toxicity in plants (Matsumoto and Motoda, 2012). Plants with strong Al tolerance usually exhibited high relative root growth (Ma et al, 2018;Badia et al, 2020). In this study, the RRG of OE-ZmAT6 transgenic maize was more than 20% higher than those in the WT.…”

Section: Overexpression Of Zmat6 Improved the Al Tolerance Of Transgementioning

confidence: 56%

A Maize ZmAT6 Gene Confers Aluminum Tolerance via Reactive Oxygen Species Scavenging

Huang

et al. 2020

Front. Plant Sci.

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Aluminum (Al) toxicity is the primary limiting factor that affects crop yields in acid soil. However, the genes that contribute to the Al tolerance process in maize are still poorly understood. Previous studies have predicted that ZmAT6 is a novel protein which could be upregulated under Al stress condition. Here, we found that ZmAT6 is expressed in many tissues and organs and can be dramatically induced by Al in both the roots and shoots but particularly in the shoots. The overexpression of ZmAT6 in maize and Arabidopsis plants increased their root growth and reduced the accumulation of Al, suggesting the contribution of ZmAT6 to Al tolerance. Moreover, the ZmAT6 transgenic maize plants had lower contents of malondialdehyde and reactive oxygen species (ROS), but much higher proline content and even lower Evans blue absorption in the roots compared with the wild type. Furthermore, the activity of several enzymes of the antioxidant system, such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), increased in ZmAT6 transgenic maize plants, particularly SOD. Consistently, the expression of ZmSOD in transgenic maize was predominant upregulated by Al stress. Taken together, these findings revealed that ZmAT6 could at least partially confer enhanced tolerance to Al toxicity by scavenging ROS in maize.

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Loss of function of Arabidopsis NADP‐malic enzyme 1 results in enhanced tolerance to aluminum stress

Cited by 15 publications

References 66 publications

Nitrate alleviates ammonium toxicity in Brassica napus by coordinating rhizosphere and cell pH and ammonium assimilation

Nitrate alleviates ammonium toxicity in Brassica napus by coordinating rhizosphere and cell pH and ammonium assimilation

Auxin is involved in arbuscular mycorrhizal fungi-promoted tomato growth and NADP-malic enzymes expression in continuous cropping substrates

A Maize ZmAT6 Gene Confers Aluminum Tolerance via Reactive Oxygen Species Scavenging

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