“…Immunocomplexes were revealed with luminol LiteAblot PLUS Enhanced Chemiluminescent Substrate (EuroClone, Pero, Italy). In control experiments, anti-RNASET2 [13, 14] and anti- Hm AIF-1 (specific for medicinal leech macrophage [4, 11]) antibodies were substituted with rabbit preimmune serum (1: 20,000). Bands were normalized by using the ImageJ software package (http://rsbweb.nih.gov/ij/download.html), and the housekeeping protein D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), used as an internal reference, was detected with a rabbit polyclonal anti-human GAPDH IgG (Proteintech, Chicago, IL, USA), diluted at 1: 2,000.…”