Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes

Moore, Alex; Chinnaiya, Kavitha; Kim, Dong Won; Brown, Sarah Jane; Stewart, Iain; Robins, Sarah C.; Dowsett, Georgina; Muir, Charlotte; Travaglio, Marco; Lewis, Jo; Ebling, Fran; Blackshaw, Seth; Furley, Andrew J.; Placzek, Marysia

doi:10.3389/fnins.2022.832961

Cited by 9 publications

(6 citation statements)

References 69 publications

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“…GFAP‐immunoreactive cells were also detected in the parenchyma from P4 and with increasing density at P10 and P20 (Figure 5E–P and inset in Figure 5I, yellow arrows). The expression profile observed at P20 was similar to that previously described in adult mice 5,24,33,34 …”

Section: Resultssupporting

confidence: 83%

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Cell proliferation and glial cell marker expression in the wall of the third ventricle in the tuberal region of the male mouse hypothalamus during postnatal development

Coutteau‐Robles

Prévot

Sharif

2023

J Neuroendocrinology

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The third ventricle (3 V) wall of the tuberal hypothalamus is composed of two types of cells; specialized ependymoglial cells called tanycytes located ventrally and ependymocytes dorsally, which control the exchanges between the cerebrospinal fluid and the hypothalamic parenchyma. By regulating the dialogue between the brain and the periphery, tanycytes are now recognized as central players in the control of major hypothalamic functions such as energy metabolism and reproduction. While our knowledge of the biology of adult tanycytes is progressing rapidly, our understanding of their development remains very incomplete. To gain insight into the postnatal maturation of the 3 V ependymal lining, we conducted a comprehensive immunofluorescent study of the mouse tuberal region at four postnatal ages (postnatal day (P) 0, P4, P10, and P20). We analyzed the expression profile of a panel of tanycyte and ependymocyte markers (vimentin, S100, connexin‐43 [Cx43], and glial fibrillary acidic protein [GFAP]) and characterized cell proliferation in the 3 V wall using the thymidine analog bromodeoxyuridine. Our results show that most changes in marker expression occur between P4 and P10, with a switch from a 3 V mostly lined by radial cells to the emergence of a tanycytic domain ventrally and an ependymocytic domain dorsally, a drop in cell proliferation and increased expression of S100, Cx43, and GFAP that acquire a mature profile at P20. Our study thus identifies the transition between the first and the second postnatal week as a critical time window for the postnatal maturation of the 3 V wall ependymal lining.

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Section: Resultssupporting

confidence: 83%

“…The expression profile observed at P20 was similar to that previously described in adult mice. 5,24,33,34…”

Section: Gfapmentioning

confidence: 99%

Cell proliferation and glial cell marker expression in the wall of the third ventricle in the tuberal region of the male mouse hypothalamus during postnatal development

Coutteau‐Robles

Prévot

Sharif

2023

J Neuroendocrinology

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“…Much less is currently known about the extrinsic factors that influence the formation of tanycytes. scRNA-seq data suggests that various morphogenic and growth factor signaling components such as Wnt7b, Ptch1, and Igfbp2 are enriched in early stages of their developmental trajectory (Kim et al, 2020), and a knockout mouse model indicates that at later timespoints, the cell adhesion molecule NrCAM plays a role in regulating postnatal tanycyte number (Moore et al, 2022). In addition, microglia may modulate tanycyte development, as these phagocytic cells have emerged as key modulators of diverse neurodevelopmental processes in many areas 10.3389/fnins.2022.1129414 of the central nervous system, including the hypothalamus (Bilimoria and Stevens, 2015;.…”

Section: Development Of Tanycytesmentioning

confidence: 99%

Developmental and functional relationships between hypothalamic tanycytes and embryonic radial glia

Fong

Kurrasch²

2023

Front. Neurosci.

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The hypothalamus is a key regulator of several homeostatic processes, such as circadian rhythms, energy balance, thirst, and thermoregulation. Recently, the hypothalamic third ventricle has emerged as a site of postnatal neurogenesis and gliogenesis. This hypothalamic neural stem potential resides in a heterogeneous population of cells known as tanycytes, which, not unlike radial glia, line the floor and ventrolateral walls of the third ventricle and extend a long process into the hypothalamic parenchyma. Here, we will review historical and recent data regarding tanycyte biology across the lifespan, focusing on the developmental emergence of these diverse cells from embryonic radial glia and their eventual role contributing to a fascinating, but relatively poorly characterized, adult neural stem cell niche.

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“…NCAM is expressed in adult mouse tanycytes (Moore et al., 2022). It is detected in all four tanycyte subsets, particularly strong expression is seen in α2 tanycyte cell bodies.…”

Section: Pars Tuberalismentioning

confidence: 99%

“…NCAM loss of function leads to both a reduced number of tanycytes and reduced expression of tanycyte‐specific cell markers (Moore et al., 2022).…”

Section: Pars Tuberalismentioning

confidence: 99%

Regulation of circulating thyroid hormone levels by hypothalamic tanycytes and hypophysial pars tuberalis‐specific cells and their morphological and gene‐ and protein‐expression changes under different photoperiods

Kameda

2023

J of Comparative Neurology

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Thyroid hormone in the hypothalamus acts as a key determinant of seasonal transitions. Thyroid hormone‐levels in the brain are mainly regulated by the hypothalamic tanycytes and pituitary pars tuberalis (PT)‐specific cells. TSHβ produced by the PT‐specific cells stimulates Dio2 expression and decreases Dio3 expression of the tanycytes. Both tanycytes and PT‐specific cells in photosensitive animals exhibit remarkable changes of morphological appearance and expressions of genes and proteins under different photoperiods. Long photoperiods induce increased gene‐ and protein‐expressions and active features. Short photoperiods cause the decreased gene‐ and protein‐expressions and inactive features. In the PT, expressions of TSHβ, common α‐subunit of glycoprotein hormones (α‐GSU), and MT1 receptor of melatonin receptors and eyes absent 3 change under different photoperiods. Diurnal rhythms of α‐GSU mRNA expression are observed in the PT of Djungarian hamsters. Hes1, Nkx2.1, and LIM homeodomain gene 2 (Lhx2) are involved in the differentiation of PT. In the hypothalamic tanycytes, expressions of Dio2, Dio3, vimentin, serine/threonine kinase 33, GPR50, Nestin, Retinoid signaling genes (retinaldehyde dehydrogenase 1, cellular retinol binding protein 1, and Stra6), monocarboxylate transporter 8, and neural cell adhesion molecule change under different photoperiods. Rax, Lhx2, Nfia/b/x, and fibroblast growth factor 10 are involved in the differentiation of tanycytes.

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Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes

Cited by 9 publications

References 69 publications

Cell proliferation and glial cell marker expression in the wall of the third ventricle in the tuberal region of the male mouse hypothalamus during postnatal development

Cell proliferation and glial cell marker expression in the wall of the third ventricle in the tuberal region of the male mouse hypothalamus during postnatal development

Developmental and functional relationships between hypothalamic tanycytes and embryonic radial glia

Regulation of circulating thyroid hormone levels by hypothalamic tanycytes and hypophysial pars tuberalis‐specific cells and their morphological and gene‐ and protein‐expression changes under different photoperiods

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