Loss of genetic integrity in artificially aged seed lots of rice (Oryza sativa L.) and common bean (Phaseolus vulgaris L.)

Dantas, Alisson F.; Fascineli, Maria Luiza; José, Solange Carvalho Barrios Roveri; Pádua, Juliano Gomes; Gimenes, Marcos Aparecido; Grisólia, César Koppe

doi:10.1016/j.mrgentox.2019.07.008

Cited by 15 publications

(8 citation statements)

References 39 publications

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“…During storage, seed vigour decreases either rapidly or slowly, which leads to dramatic economic losses and a loss of genetic diversity (Dantas et al . 2019). Safflower ( Carthamus tinctorius , Asteraceae) is an important commercial crop that is cultivated as oilseed livestock feed, dye source and medicinal purposes (Ekin, 2005).…”

Section: Introductionmentioning

confidence: 99%

Integrated proteome and lipidome analysis of naturally aged safflower seeds varying in vitality

et al. 2021

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Seed ageing has an important effect on germination and productivity. During natural ageing, seed vigour decreases rapidly but, to date, the molecular mechanisms underlying this decrease have not been fully elucidated. Using omics, some of the details regarding seed vigour decline during natural ageing might be elucidated through integrated analysis. Safflower seed germination and physio‐biochemical changes during natural ageing (stored for 4, 16 and 28 months) were determined. Proteome and lipidome profiling during natural seed ageing was performed, and the differentially expressed proteins and lipid metabolite species analysed. The surface and internal structures of cotyledons were observed. An integrating analysis of the proteome and lipidome was also carried out. Natural seed ageing significantly decreased safflower seed germination and vigour. 4,184 proteins and 1,193 lipids were quantified, both of which show huge differences among the different naturally aged seeds. The surface of the cotyledons collapsed and cracked, and the oil bodies become looser during natural ageing. The total content of DAG and PA increased, while the content of TAG and PL (PC, PE, PS, PI and PL) significantly decreased during seeds ageing. Two lipase genes (HH‐026818‐RA and HH‐025320) likely participated in this degradation of lipids. We conclude that the enzymes that participate in glycerolipid metabolism and fatty acid degradation probably lead to the degradation of oil bodies (TAG) and membrane lipids (PC, PE, PS, PI, PG) and, ultimately, destroy the structure, causing a decline in seed vigour during natural seed ageing.

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Section: Introductionmentioning

confidence: 99%

Integrated proteome and lipidome analysis of naturally aged safflower seeds varying in vitality

et al. 2021

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“…To induce artificial aging, seeds are exposed to a high temperature (approximately 41 • C) and high relative humidity (RH; 100%) for a designated duration. Then, a germination test is carried out to examine changes in seed vigor (Arc et al, 2011;Dantas et al, 2019). Aging affects several physiological and biochemical processes within the seed, including reactive oxygen species (ROS) metabolism, malondialdehyde (MDA) content, lipid peroxidation, membrane permeability (Mira et al, 2011;Chen et al, 2016), nucleic acid stability, and protein expression and modification (Rajjou and Debeaujon, 2008;Kranner et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice

et al. 2020

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MicroRNAs (miRNAs) play important roles in various aspects of plant physiology and metabolism. The expression level of miR164c is negatively correlated with seed vigor in rice ( Oryza sativa L.); however, the mechanism of seed vigor regulation by miR164c remains unknown. Anti-aging capacity is an important indicator of seed vigor. Here, we report an miR164c-guided gene/protein interaction network that regulates the anti-aging ability of rice seeds. Seeds of the wild-type (WT) rice cultivar “Kasalath” and its transgenic derivatives, miR164c-silenced line (MIM164c) and miR164c overexpression line (OE164c), with significant differences in anti-aging capacity, showed significant differences in gene and protein expression levels. The differentially expressed genes (DEGs) or proteins were significantly enriched in six metabolic functional categories related to seed vigor, including “stress response,” “protein processing in endoplasmic reticulum (ER),” “embryo development,” “serine-type endopeptidase inhibitor,” “energy metabolism,” and “other.” Differences in the expression levels of genes or proteins related to energy metabolism, serine endopeptidase, and stress response in seeds under normal storage conditions may be associated with anti-aging capacity. The results of gene/protein interaction analyses suggest that miR164c first targets PSK5 , and the PSK5 protein then interacts with the ubiquitin-associated gene RPS27AA , which simultaneously impacts the genes/proteins in the six above-mentioned functional categories. Expression levels of some of the key genes and proteins in the interaction network were verified by real-time fluorescence quantitative PCR (RT-qPCR) and multiple reaction monitoring mass spectrometry (MRM-MS), respectively. Thus, the present study provides new insights into the miRNA-mediated gene and protein interaction network that regulates seed vigor.

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“…The vagrant chromosomes were the most frequent damages followed by nuclear buds. Nuclear damage also has been seen in artificially aged seeds of A. angustifolia (Fontes et al, 2001), P. sativum (Khan et al, 2003), O. sativa and P. vulgaris (Dantas et al, 2019).…”

Section: Cytogenetic Characterizationmentioning

confidence: 99%

“…Despite the importance of acid nucleic integrity for seed germination that issue has received relatively little attention in the literature (Kranner et al, 2011). The DNA integrity loss due to aging has been in general evaluated using primary roots or seedlings and, in general, minor differences among different aged seed lots have been found (El-Maarouf-Bouteau et al 2011;Donà et al, 2013;Menezes et al, 2014;Dantas et al, 2019). Kranner et al (2011) evaluated DNA degradation among artificially aged pea (Pisum sativum) seed samples using DNA laddering and observed different levels of degradation and DNA repair system efficiency among samples.…”

Section: Introductionmentioning

confidence: 99%

Research Article Evaluation of DNA integrity as a potential marker to detect aging in soybean (<i>Glycine</i> <i>max</i>) seeds

Lopes¹,

Dantas²,

Pádua³

et al. 2021

Genet. Mol. Res.

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Loss of genetic integrity in artificially aged seed lots of rice (Oryza sativa L.) and common bean (Phaseolus vulgaris L.)

Cited by 15 publications

References 39 publications

Integrated proteome and lipidome analysis of naturally aged safflower seeds varying in vitality

Integrated proteome and lipidome analysis of naturally aged safflower seeds varying in vitality

Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice

Research Article Evaluation of DNA integrity as a potential marker to detect aging in soybean (<i>Glycine</i> <i>max</i>) seeds

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