Loss of heterozygosity and immunoexpression of PTEN in oral epithelial dysplasia and squamous cell carcinoma

Chaves, Filipe Nobre; Bezerra, Thâmara Manoela Marinho; Moraes, Débora Chaves; Costa, Sara Ferreira dos Santos; Silva, Paulo Goberlanio Barros; Alves, Ana Paula Negreiros Nunes; Costa, Fábio Wildson Gurgel; Bernardes, Vanessa Fátima

doi:10.1016/j.yexmp.2019.104341

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…PTEN, a multifunctional tumor suppressor with protein and lipid phosphatase activity, is localized at 10q23.3 and composed of nine exons. It is closely associated with the development of many tumors, and it is also very important in colorectal cancer (12,13,14).…”

Section: Discussionmentioning

confidence: 99%

“…There is also evidence that PTEN plays an important role in the in ammation-dysplasia-cancer sequence and may be an important regulator of ulcerative colitis-associated colorectal cancer. PTEN may inhibit tumor development by negatively regulating the PI3K/Akt pathway, decreasing the expression of Akt and Ki67, and increasing the expression of Caspase3, thereby inhibiting colonic in ammation and cell proliferation and promoting apoptosis (12,15,16). Therefore, PTEN may be a new target for UCassociated colorectal cancer prevention and treatment.…”

Section: Discussionmentioning

confidence: 99%

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Genetic relationship between colorectal cancer and ulcerative colitis revealed by bioinformatics

Huang

Lan

Wang

et al. 2023

Preprint

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Objective To investigate the bioinformatics analysis methods of genes associated with colorectal cancer in ulcerative colitis. Methods We employed the intersection of the differential genes between UC and healthy controls, differential genes between UC dysplasia and UC, and the differential genes between UC dysplasia and healthy controls in GSE47908 to obtain overlapping genes and validated their accuracy in the TCGA dataset of COAD and GSE40967 to screen risk genes. The GSE110224/GSE113513 dataset of CODA, and the UC and COAD-related dataset GSE3629 were integrated for WGCNA analysis after normalizing the data. NOMO plot analysis was performed using the expression of overlapping genes of modular and risk genes in GSE47908 with UC dysplasia and UC. Results 1576 overlapping genes were detected after screening for differential genes, which were validated in the TCGA and GSE datasets of colorectal cancer to construct a prognostic model. It was found that all P-values were less than 0.05 after survival analysis and less than 0.05 for progression-free survival, and the area under the risk score curve of the ROC curve was 0.894, which could be more accurate as a predictor of patient prognostic indicators. Then, WGCNA analysis was performed on UC, COAD and healthy controls to obtain five modular genes and intersected with overlapping genes to obtain 490 overlapping genes, and NOMO plotting by the LASSO algorithm to obtain seven key genes to predict the risk score of UC progression to COAD. Conclusion We screened seven gene indicators that could be used as key biomarkers of colorectal cancer susceptibility in patients with ulcerative colitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic relationship between colorectal cancer and ulcerative colitis revealed by bioinformatics

Huang

Lan

Wang

et al. 2023

Preprint

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“…DNA aneuploidy 29 and chromosome aberrations30 are commonly used to detect field cancerization at the DNA level. Several markers (p53, Ki-67, 31 cytokeratin fragments 21-1, 28 variations in nucleolar organizer regions, 32 phosphatases and tensin homolog deleted on chromosome 10 allelic loss, 33 DEK overexpression, 34 micro RNA [hsa-miR-221, hsa-miR-21, hsa-miR-135b, and hsa-miR-29c] detection, 35 ATP-binding cassette subfamily G member 2, 36 MutL protein homolog 1, methylguanine-methyltransferase methylation, 37 interferonstimulated gene 15, 38 aldehyde dehydrogenase, Notch1, 39 and Bmi1 40 ) have been identified in pre-cancerization transformation into oral cancer, stimulating the cell cycle and promoting DNA replication (Figure 3).…”

Section: Markers Of Field Cancerizationmentioning

confidence: 99%

Oral field cancerization: Genetic profiling for a prevention strategy for oral potentially malignant disorders

et al. 2023

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Background: Oral cancer therapy, such as radiation or surgical treatment, has pernicious long-term effects that patients suffer throughout their life, the disability being considerable with delayed diagnosis. It is well known that many oral cancers develop from oral potentially malignant disorders (OPMDs). Patients diagnosed with OPMDs may have an increased risk of developing cancer anywhere in the oral cavity. Early detection and intervention could be essential prevention strategies to inhibit oral cancer progression. OPMDs may not immediately develop into carcinoma. However, this condition provides a “field” of specific abnormalities wherein evolving altered genetic cells can be explained with the “field cancerization” concept. Purpose: This review aims to describe the “field cancerization” concept in oral cancer and OPMD, which is expected to contribute to a better clinical management strategy for oral cancer prevention. Review: “Oral field cancerization” describes oral cancers that develop in multifocal areas of pre-cancerous changes. It can be found as histologically abnormal tissue surrounding the tumor, suggesting that oral cancer often consists of multiple independent lesions. Conclusion: The oral field cancerization concept should prompt healthcare professionals to remind their patients that frequent oral examination with histological studies and molecular testing is mandatory for those at high risk of developing malignancies.

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“…The presence of a brown color in the cytoplasm and membrane was considered positive labeling. According to IHC evaluation methods adapted from previous studies (22), the presence and degree of RNF215 staining were divided into three categories: Negative expression, weak expression, and overexpression. The chi-square test or Fisher's test was conducted to identify the association between RNF215 expression and clinicopathological characteristics.…”

Section: Immunohistochemistr Y (Ihc)mentioning

confidence: 99%

Ring finger protein 215 is a potential prognostic biomarker involved in immune infiltration and angiogenesis in colorectal cancer

Liu

et al. 2023

Biomed Rep

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The prognostic value of ring finger protein 215 (RNF215) in colorectal cancer (CRC) is unclear. Herein, the present study aimed to investigate the precise value of RNF215 based on CRC datasets from The Cancer Genome Atlas (TCGA) and clinical cases. CRC patient data was collected from TCGA and clinical samples from the Department of Pathology, Shanghai Fifth People's Hospital, Fudan University (Shanghai, China). Logistic regression analysis was used to investigate the correlations between RNF215 and clinicopathological characteristics. The predictive value of RNF215 for the clinical outcome of CRC was determined using Kaplan-Meier curves and Cox regression. Gene set enrichment analysis (GSEA), single-sample GSEA (ssGSEA), and angiogenesis analysis were also conducted to investigate the biological role of RNF215. Immunohistochemistry was conducted to validate the results. The results of the present study confirmed that RNF215 protein expression was significantly associated with age, lymphatic invasion, and overall survival (OS). Univariate analysis showed that upregulation of RNF215 in CRC was significantly associated with age and lymphatic invasion. Kaplan-Meier survival analysis revealed that high RNF215 expression predicted poorer OS and disease-specific survival. A total of nine experimentally detected RNF215-binding proteins were identified with the STRING tool and Cytoscape software. GSEA suggested that RNF215 was associated with several important pathways involved in tumor occurrence, including the Kyoto Encyclopedia of Genes and Genomes MAPK signaling pathway and the WikiPathway RAS signaling pathway. ssGSEA confirmed that RNF215 was significantly expressed in natural killer cells, CD8 T cells and T helper cells. Angiogenesis analysis revealed that numerous angiogenesis-related genes had the same expression trend as RNF215 in CRC. The immunostaining results indicated that RNF215 expression was significantly higher in CRC tissues than in corresponding normal tissues. In conclusion, increased RNF215 expression may be a potential molecular marker predictive of poor survival and a treatment target in CRC. In addition, RNF215 may participate in the formation of CRC through a variety of signaling pathways.

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Loss of heterozygosity and immunoexpression of PTEN in oral epithelial dysplasia and squamous cell carcinoma

Cited by 10 publications

References 29 publications

Genetic relationship between colorectal cancer and ulcerative colitis revealed by bioinformatics

Genetic relationship between colorectal cancer and ulcerative colitis revealed by bioinformatics

Oral field cancerization: Genetic profiling for a prevention strategy for oral potentially malignant disorders

Ring finger protein 215 is a potential prognostic biomarker involved in immune infiltration and angiogenesis in colorectal cancer

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