Loss of Immunologic Reactivities of Australia Antigen After Incubation with Bacteria

Mazzur, Scott; Corbett, John J.; Blumberg, B. S.

doi:10.3181/00379727-142-37016

Cited by 16 publications

(10 citation statements)

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“…All seven sera were heavily contaminated with bacteria. It is possible that some determinants of HB,Ag were partially digested in these samples by exoenzymes of bacteria, as was shown by Mazzur et al [15]; they incubated sera containing HB,Ag at 37 "C for 4 and 5 days with filtrates of Pseudornonas aeruginosa and Escherichia coli and found a loss of antigenic reactvity of HB,Ag in ID. Guinea pigs, however, were still able to respond to the d specificity of the contaminated Bulgarian samples.…”

Section: Discussionmentioning

confidence: 88%

Subtypes of HBsAg in Eastern and South‐Eastern Europe

Madaliński¹,

Holland²,

Moraczewska³

et al. 1977

Vox Sanguinis

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A total of 481 serum samples was collected from asymptomatic carriers of HBSAg as well as some patients with acute and chronic hepatitis, type B. The sera were obtained mainly from blood banks in the following countries: Poland, Hungary, Romania, Bulgaria, Yugoslavia, and the European part of the Soviet Union. Asymptomatic carriers of HBsAg from Poland and Hungary showed a preponderance of adw subtype over ayw subtype (Poland -80.7% adw, Hungary -72.2% adw). Carriers from the remaining countries showed the reverse situation: Bulgaria -71.5% ayw, Yugoslavia -79.0% ayw, Romania -82.8% ayw, Soviet Union -87.5% ayw. No individuals were found among these indigenous populations to have the adr or ayr subtypes but two carriers appeared to have the unusual subtype adyw.

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Section: Discussionmentioning

confidence: 88%

Subtypes of HBsAg in Eastern and South‐Eastern Europe

Madaliński¹,

Holland²,

Moraczewska³

et al. 1977

Vox Sanguinis

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“…This determinant occurs only in the Far East, more often in connection with the determinant d than y [2], Holland et al [10] have found that the adw subtype occurs in 87% of cases of HBsAg carriership in the USA. In contrast, these authors noted that in acute hepatitis is possible that some determinants of HBsAg were partially digested in these samples by exoenzymes of bacteria, as was shown by Mazzur et al [15]; they incubated sera con taining HBsAg at 37 °C for 4 and 5 days with filtrates of Pseudomonas aeruginosa and Escherichia coli and found a loss of antigenic reactvity of HBsAg in ID. Guinea pigs, however, were still able to respond to the d specificity of the contaminated Bul garian samples.…”

Section: Discussionmentioning

confidence: 93%

Subtypes of HBsAg in Eastern and South-Eastern Europe

Madaliński¹,

Holland²,

Moraczewska³

et al. 1977

Vox Sanguinis

View full text Add to dashboard Cite

A total of 481 serum samples was collected from asymptomatic carriers of HBsAg as well as some patients with acute and chronic hepatitis, type B. The sera were obtained mainly from blood banks in the following countries; Poland, Hungary, Romania, Bulgaria, Yugoslavia, and the European part of the Soviet Union. Asymptomatic carriers of HBsAg from Poland and Hungary showed a preponderance of adw subtype over ayw subtype (Poland -80.7% adw, Hungary -72.2% adw). Carriers from the remaining countries showed the reverse situation: Bulgaria -71.5% ayw, Yugoslavia -79.0% ayw, Romania -82.8% ayw, Soviet Union -87.5% ayw. No individuals were found among these indigenous populations to have the adr or ayr subtypes but two carriers appeared to have the unusual subtype adyw.

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“…In general, a rough equivalence has been found between a and d, or a and y; but in some cases the subspecificity appears to be relatively weak, or even undetectable. However, it is known that HB Ag reactivity can be abolished in bacterially contaminated sera [9]; and Schober et al [10] have shown that reduction of disulfide bonds by 2-mercaptoethanol causes a preferential loss of d or y reactivity, as compared with a. Some preliminary experiments of our own, in collaboration with Dr. G o r d o n D reesman, indicate that incuba tion at 37° of apparently uncontaminated HB Ag+ sera results in a loss of d or y antigenic activity within 2-3 weeks, at a time when the a still seems to be fully functional.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Antigen with Both d and y Specificities on the Same Particles

Nordenfelt¹,

Bouvier²

1973

Intervirology

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A patient carrying hepatitis B antigen with both d and y specificities on the same particles (diplotypic HB Ag) has been followed over a period of two years, with examination of repeated blood samples.Titration studies on these sera consistently showed a stronger reaction and higher titer of specificities a and w, as compared with specificities d and y. These relationships differed from those seen in sera containing HB Ag of the usual ‘simple’ kind, whether of subtype D or subtype Y, in which the reactions of a and d, or a and y, paralleled each other closely. It is hypothesized that the most likely explanation for the appearance of this diplotypic HB Ag is a double infection with hepatitis B viruses of subtypes D and Y, resulting in a combination of the adw and ayw genomes, and the formation of a new viral genome possessing genes for both the d and the y specificities.

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Loss of Immunologic Reactivities of Australia Antigen After Incubation with Bacteria

Cited by 16 publications

References 0 publications

Subtypes of HBsAg in Eastern and South‐Eastern Europe

Subtypes of HBsAg in Eastern and South‐Eastern Europe

Subtypes of HBsAg in Eastern and South-Eastern Europe

Hepatitis B Antigen with Both d and y Specificities on the Same Particles

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