Loss of MBD2 ameliorates LPS‐induced alveolar epithelial cell apoptosis and ALI in mice via modulating intracellular zinc homeostasis

Liu, Jiqiang; Yao, Shuo; Jia, Jingsi; Chen, Zhifeng; Yuan, Yu; He, Yi; Wasti, Binaya; Duan, Wentao; Li, Danhong; Wang, Guyi; Jia, Aijun; Sun, Wen-Jin; Qiu, Shuangfa; Ma, Liwei; Li, Jianmin; Liu, Yi; Zheng, Jianfei; Xiang, Xudong; Zhang, Xiufeng; Liu, Shaokun; He, Zhijun; Peng, Zhenyu; Zhang, Hongliang; Zhang, Dongshan; Xiao, Bing

doi:10.1096/fj.202100924rr

Cited by 10 publications

(10 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BALF was collected, and BALF protein levels were measured in accordance with methods used in our previous study (Liu et al. 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Lung edoema was assessed using the wet/dry weight ratio (Liu et al. 2022 ). Briefly, the upper lobe of the left lung was separated and weighed (wet weight, W).…”

Section: Methodsmentioning

confidence: 99%

“…LPS ( Escherichia coli LPS O55:B5; Sigma–Aldrich, St. Louis, MO) was first dissolved in a stock solution (5 mg/mL), diluted to a final concentration of 500 ng/mL, and treated for 24 h (Liu et al. 2022 ). In certain groups, cells were treated with FA (0.1 μM) or Fe (ferroptotic inducer, Fe-citrate, 5 M, Selleck Chemicals, Houston, TX) (Wang et al.…”

Section: Methodsmentioning

confidence: 99%

“…qPCR experiments were carried out as previously described (Liu et al. 2022 ). In brief, total RNA extraction was implemented employing the TRIzol ® kit (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

“…Cell death is a critical cause of alveolar epithelial injury, and our recent work revealed that apoptosis of alveolar epithelial cells is a major reason for alveolar epithelial cell loss (Liu et al. 2022 ). However, ferroptosis, another form of programmed cell death, was recently reported to be of great significance in the process of sepsis and ALI (Zhu et al.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Ferulic acid alleviates alveolar epithelial barrier dysfunction in sepsis-induced acute lung injury by activating the Nrf2/HO-1 pathway and inhibiting ferroptosis

Tang

Liu

Yao

et al. 2022

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Ferulic acid (FA) has antioxidative and anti-inflammatory effects, and is a promising drug to treat sepsis. Objective To study the therapeutic effect of FA in sepsis-induced acute lung injury (ALI) and its underlying mechanisms. Materials and methods The caecal ligation and puncture (CLP) manoeuvre was applied to establish a murine model of sepsis-induced ALI, and female BALB/c mice (6 mice per group) were subjected to 100 mg/kg FA or 0.8 mg/kg ferrostatin-1 (Fer-1, ferroptosis inhibitor) treatment to clarify the role of FA in preserving alveolar epithelial barrier function and inhibiting ferroptosis. Lipopolysaccharide (LPS; 500 ng/mL)-induced cell models were prepared and subjected to FA (0.1 μM), sh-Nrf2, and Fe (Fe-citrate, ferroptosis inducer; 5 M) treatment to study the in vitro effect of FA on LPS-induced alveolar epithelial cell injury and the role of the Nrf2/HO-1 pathway. Results We found that FA decreased the lung injury score (48% reduction), lung wet/dry weight ratio (33% reduction), and myeloperoxidase activity (58% reduction) in sepsis-induced ALI. Moreover, FA inhibited ferroptosis of alveolar epithelial cells and improved alveolar epithelial barrier dysfunction. The protective role of FA against alveolar epithelial barrier dysfunction could be reversed by the ferroptosis inducer Fe-citrate, suggesting that FA alleviates alveolar epithelial barrier dysfunction by inhibiting ferroptosis. Mechanistically, we found that FA inhibited ferroptosis of alveolar epithelial cells by activating the Nrf2/HO-1 pathway. Conclusion Collectively, our data highlighted the alleviatory role of ferulic acid in sepsis-induced ALI by activating the Nrf2/HO-1 pathway and inhibiting ferroptosis, offering a new basis for sepsis treatment.

show abstract

“…BALF was collected, and BALF protein levels were measured in accordance with methods used in our previous study (Liu et al. 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Lung edoema was assessed using the wet/dry weight ratio (Liu et al. 2022 ). Briefly, the upper lobe of the left lung was separated and weighed (wet weight, W).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…qPCR experiments were carried out as previously described (Liu et al. 2022 ). In brief, total RNA extraction was implemented employing the TRIzol ® kit (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations