Loss of <scp>PPM</scp>1F expression predicts tumour recurrence and is negatively regulated by miR‐590‐3p in gastric cancer

Zhang, Jing; Jin, Ming; Chen, Xiaoyu; Zhang, Rui; Huang, Yanxia; Liu, Hui; Zhu, Jingde

doi:10.1111/cpr.12444

Cited by 27 publications

(28 citation statements)

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“…PPM1F, expressed in a variety of tumor cell lines, facilitates cell motility and invasiveness [ 4 ] and breast carcinogenesis by inactivating p53 signaling [ 5 ], promotes tumor metastasis via MAPK signaling and exosome cytokine secretion [ 6 ], and represses cell apoptosis through the TAK1-IKK-NF-κB pathway [ 7 ]. However, our previous study showed that PPM1F is downregulated in gastric cancer (GC), and low expression of PPM1F is associated with poor survival in patients with GC [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

CircSLC3A2 functions as an oncogenic factor in hepatocellular carcinoma by sponging miR-490-3p and regulating PPM1F expression

et al. 2018

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BackgroundNon-coding RNAs (ncRNAs) have been reported to participate in tumor progression by regulating gene expression. Previous studies showed that protein phosphatase Mg2+/Mn2+ dependent 1F (PPM1F) acts a dual role in cancer growth and metastasis. But, the underlying mechanisms by which ncRNAs regulate PPM1F expression in hepatocellular carcinoma (HCC) are poorly understood.MethodsThe association between PPM1F or miR-490-3p expression and clinicopathological features and prognosis in patients with HCC was analyzed by TCGA RNA-sequencing data. CircSLC3A2 was identified to bind with miR-490-3p by bioinformatic analysis, and the binding sites between miR-490-3p and PPM1F or circSLC3A2 were confirmed by dual luciferase report and RNA immunoprecipitation (RIP) assays. The localization and clinical significance of miR-490-3p and circSLC3A2 in patients with HCC were investigated by fluorescence in situ hybridization (FISH). MTT, Agar, and Transwell assays were conducted to evaluate the effects of miR-490-3p or circSLC3A2 on cell proliferation and invasive potential.ResultsThe expression of PPM1F or miR-490-3p was associated with poor survival and tumor recurrence, and acted as an independent prognostic factor in patients with HCC. Re-expression of miR-490-3p inhibited HCC cell proliferation and invasion by targeting PPM1F, but its inhibitor reversed these effects. Moreover, circSLC3A2, predominantly localized in the cytoplasm, exhibited an oncogenic role by sponging miR-490-3p and regulating PPM1F expression, and harbored a positive correlation with poor survival in patients with HCC.ConclusionCircSLC3A2 acts as an oncogenic factor in HCC by sponging miR-490-3p and regulating PPM1F expression.Electronic supplementary materialThe online version of this article (10.1186/s12943-018-0909-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

CircSLC3A2 functions as an oncogenic factor in hepatocellular carcinoma by sponging miR-490-3p and regulating PPM1F expression

et al. 2018

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“…However, the function of miR-590-3p in different tumors remains controversial. MiR-590-3p relatively high expressed in gastric carcinoma (GC) samples and was associated with tumor relapse in GC patients by direct regulating PPM1F [16] . MiR-590-3p motivated colon cancer cell growth and metastasis via Wnt/β-catenin signaling pathway and Hippo pathway [6; 17] and enhanced ovarian cancer proliferation and metastasis via targeting Cyclin G2, FOXA2 and FOXO3 [18; 19] .…”

Section: Disscussionmentioning

confidence: 99%

Hsa-MiR-590-3p promotes the malignancy progression of pancreatic ductal carcinoma cell by inhibiting the expression of p27 and PPP2R2A via G1/S cell cycle pathway

Shi

Sheng

Chen

et al. 2020

Preprint

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Background: miR-590-3p plays an important role in the occurrence and development of many cancers, but its role in pancreatic cancer has not been reported. Objective: Investigate the effect and target genes of miR-590-3p in pancreatic cancer, so as to provide new diagnostic and therapeutic ideas for pancreatic cancer. Methods: qRT-PCR, MTT, clonal formation assay, flow cytometry and transwell assay were used to detect malignant cell behaviors. TargetScan (http://www.targetscan.org) was used to predict the binding region of miR-590-3p to target genes. Immunohistochemistry and Western blot were used to detect protein expression.Results: hsa-miR-590-3p expressed significantly higher in PC tissues than paired normal pancreas and its expression level was associated with PC tumor size (P=0.042) and preoperative CA19-9 level (P=0.046). Its overexpression promoted PC cell proliferation, invasion and migration following with the p27 and PPP2R2A protein down-regulation, and vice versa. Dual-luciferase reporter assay confirmed that p27 and PPP2R2A were direct target genes of miR-590-3p. Overexpression of P27 and PPP2R2A reversed the promoting effect of miR-590-3p on cell proliferation, migration and invasion. Conclusion: MiR-590-3p promote the development of pancreatic cancer by directly downregulated p27 and PPP2R2A via the G1/S cell cycle pathway.

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“…Every effort has been made to improve the pathogenesis and therapeutic strategies of GC [2], such as eradication of Helicobacter pylori [3], but GC is generally diagnosed at an advanced stage and its prognosis is poor due to tumor invasiveness [4]. Substantial evidence shows that deregulated expression of non-coding RNAs (ncRNAs) is linked to the progression of GC [5,6]. Thus, it is indispensable to identify novel biomarkers for early detection of GC.…”

Section: Introductionmentioning

confidence: 99%

CircDUSP16 promotes the tumorigenesis and invasion of gastric cancer by sponging miR-145-5p

et al. 2019

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Background Circular RNAs (circRNAs) as a novel subgroup of non-coding RNAs act a critical role in the pathogenesis of gastric cancer (GC). However, the underlying mechanisms by which hsa_circ_0003855 (circDUSP16) contributes to GC are still undocumented. Materials The differentially expressed circRNAs were identified by GEO database. The association of circDUSP16 or miR-145-5p expression with clinicopathological features and prognosis in GC patients was analyzed by FISH and TCGA-seq data set. Loss-and gain-of-function experiments as well as a xenograft tumor model were performed to assess the role of circDUSP16 in GC cells. circDUSP16-specific binding with miR-145-5p was confirmed by bioinformatic analysis, luciferase reporter, and RNA immunoprecipitation assays. Results The expression levels of circDUSP16 were markedly increased in GC tissue samples and acted as an independent prognostic factor of poor survival in patients with GC. Knockdown of circDUSP16 repressed the cell viability, colony formation, and invasive potential in vitro and in vivo, but ectopic expression of circDUSP16 reversed these effects. Moreover, circDUSP16 possessed a co-localization with miR-145-5p in the cytoplasm, and acted as a sponge of miR-145-5p, which attenuated circDUSP16-induced tumor-promoting effects and IVNS1ABP expression in GC cells. MiR-145-5p had a negative correlation with circDUSP16 expression and its low expression was associated with poor survival in GC patients. Conclusions CircDUSP16 facilitates the tumorigenesis and invasion of GC cells by sponging miR-145-5p, and may provide a novel therapeutic target for GC.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Loss of PPM1F expression predicts tumour recurrence and is negatively regulated by miR‐590‐3p in gastric cancer

Abstract: PPM1F may function as a suppressive factor and is negatively regulated by miR-590 in GC.

Cited by 27 publications

References 31 publications

CircSLC3A2 functions as an oncogenic factor in hepatocellular carcinoma by sponging miR-490-3p and regulating PPM1F expression

CircSLC3A2 functions as an oncogenic factor in hepatocellular carcinoma by sponging miR-490-3p and regulating PPM1F expression

Hsa-MiR-590-3p promotes the malignancy progression of pancreatic ductal carcinoma cell by inhibiting the expression of p27 and PPP2R2A via G1/S cell cycle pathway

CircDUSP16 promotes the tumorigenesis and invasion of gastric cancer by sponging miR-145-5p

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