Loss of UHRF2 Is Associated With Non-small Cell Lung Carcinoma Progression

Chun, Jennifer; Xiong, Dian; Li, Haoran; Jiang, Jiahao; Qi, Jianchao; Ding, Jianyong

doi:10.7150/jca.25876

Cited by 13 publications

(7 citation statements)

References 32 publications

(42 reference statements)

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“… 29 The abundance of UHRF2 in lung cancer was lower, and UHRF2 knockout in A549 and 95-D cell lines enhanced the proliferation, invasion and migration of NSCLC cells, while overexpression of UHRF2 inhibited the proliferation, invasion and migration of NSCLC cells. 30 Our results showed that this gene acted as an oncogene in colon cancer, which was consistent with other studies. 31 , 32 UHRF2 was upregulated at both transcription and translation levels in CRC tumor tissue.…”

Section: Discussionsupporting

confidence: 93%

The Role of RNA Methylation Modification Related Genes in Prognosis and Immunotherapy of Colorectal Cancer

Lin¹,

Chen²,

Ye³

et al. 2023

IJGM

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Background Researches showed RNA methylation genes can affect the prognosis of tumors. Thus, the study aimed to comprehensively analyze the effects of RNA methylation regulatory genes in prognosis and treatment of colorectal cancer (CRC). Methods Prognostic signature associated with CRCs were constructed by differential expression analysis, Cox and Least Absolute Shrinkage and Selection Operator (LASSO) analyses. Receiver operating characteristic (ROC) and Kaplan–Meier survival analyses were used to validate the reliability of the developed model. Gene Ontology (GO), Gene set variation analysis (GSVA), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed for functional annotation. Finally, normal and cancerous tissue were collected to validate gene by quantitative real-time PCR (qRT-PCR). Results A prognostic risk model based on leucine rich pentatricopeptide repeat containing (LRPPRC) and ubiquitin-like with PHD and ring finger domains 2 (UHRF2) was constructed and relevant to the overall survival (OS) of CRC. Functional enrichment analysis revealed that collagen fibrous tissue, ion channel complex and other pathways were significantly enriched, which might help explain the underlying molecular mechanisms. There were significant differences in ImmuneScore, StromalScore, ESTIMATEScore between high- and low-risk groups (p < 0.05). Ultimately, qRT-PCR validation showed that a significant upregulation in the expression of LRPPRC and UHRF2 in cancerous tissue, which verified the effectiveness of our signature. Conclusion In conclusion, 2 prognostic genes (LRPPRC and UHRF2) related to RNA methylation were identified by bioinformatics analysis, which might supply a new insight into the treatment and evaluation of CRC.

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Section: Discussionsupporting

confidence: 93%

The Role of RNA Methylation Modification Related Genes in Prognosis and Immunotherapy of Colorectal Cancer

Lin¹,

Chen²,

Ye³

et al. 2023

IJGM

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“…A total of 200 paraffin-embedded NSCLC tumours and matched peritumours were constructed tissue micro-array (TMA) as previously described 16. EI2BL antibody (Abcam, ab187894) were used to detect density of expression in the TMA.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of EI2BL promoted human non-small cell lung cancer progression by inducing cell EMT phenotype

Qiu

Gao

et al. 2019

J Clin Pathol

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AimsTo unveil the role of EI2BL in non-small cell lung cancer (NSCLC) and the relationship between expression of EI2BL and the prognosis of patients with NSCLC.MethodsImmunohistochemistry (IHC), western blot analysis, immunofluorescence and real-time quantitative PCR (RT-qPCR) were used to evaluate EI2BL protein and mRNA levels in NSCLC and corresponding peritumour tissues. Cell Counting Kit-8, transwell assay and wound healing assay were used to analyse the abilities of cell proliferation, invasion and migration. In addition, the analysis of epithelial-mesenchymal transition (EMT) markers was also assessed by western blot analysis, RT-qPCR and immunofluorescence. Tissue micro-array analysis of 200 NSCLC cases was used to assess the relationship between EI2BL expression and clinicopathological characteristics. Moreover, the prognostic role of EI2BL in 200 patients with NSCLC was evaluated by Cox regression models and Kaplan-Meier methods.ResultsElevated EI2BL expression was more common in NSCLC tissues than paired peritumour tissues in both mRNA and protein level. EI2BL promoted the proliferation, invasion and migration of NSCLC cells. In addition, aberrant EI2BL expression might modulate the expression of key molecules of EMT by ERK1/2 signal pathway. The expression of EI2BL was significantly associated with tumour stage, lymph node metastasis and tumour size. Moreover, higher expression of EI2BL in patients with NSCLC had a poor overall survival rate.ConclusionsOur study illustrated that elevated expression of EI2BL promoted NSCLC cell proliferation, migration and invasion and EI2BL overexpression may be a reliable biomarker of poor prognosis.

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“…For example, in the study of human esophageal squamous cell carcinoma, low abundance of UHRF2 was found to be associated with vascular invasion of squamous cell carcinoma and was an independent prognostic factor for poor prognosis [29]. The abundance of UHRF2 in lung cancer was lower, and UHRF2 knockout in A549 and 95-D cell lines enhanced the proliferation, invasion and migration of NSCLC cells, while overexpression of UHRF2 inhibited the proliferation, invasion and migration of NSCLC cells [30]. Our results show that this gene acts as an oncogene in colon cancer, which is consistent with other studies [31,32].…”

Section: Discussionmentioning

confidence: 99%

The Role of RNA Methylation Modification Related Genes in Prognosis and Immunotherapy of Colorectal Cancer

Lin

Chen

et al. 2022

Preprint

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This study aimed to analyze the effects of RNA methylation regulatory genes in prognosis and treatment of CRC. Prognostic signature associated with CRCs were constructed by differential expression analysis, Cox and LASSO analyses. ROC and Kaplan-Meier survival analyses were used to validate the reliability of the developed model. Finally, normal and cancerous tissue were collected to validate gene by qRT-PCR. A prognostic risk model based on LRPPRC and UHRF2 was constructed and relevant to the OS of CRC. Ultimately, PCR validation showed that a significant upregulation in the expression of LRPPRC and UHRF2 in cancerous tissue. A prognostic risk model based on LRPPRC and UHRF2 was successfully built, and the model could predict the immunotherapy efficacy of CRC.

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Loss of UHRF2 Is Associated With Non-small Cell Lung Carcinoma Progression

Cited by 13 publications

References 32 publications

The Role of RNA Methylation Modification Related Genes in Prognosis and Immunotherapy of Colorectal Cancer

The Role of RNA Methylation Modification Related Genes in Prognosis and Immunotherapy of Colorectal Cancer

Overexpression of EI2BL promoted human non-small cell lung cancer progression by inducing cell EMT phenotype

The Role of RNA Methylation Modification Related Genes in Prognosis and Immunotherapy of Colorectal Cancer

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