Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung

Ikezawa, Maiko; Tajika, Yuki; Ueno, Hitoshi; Murakami, Tohru; Inoue, Naokazu; Yorifuji, Hiroshi

doi:10.1002/dvdy.24618

Cited by 12 publications

(13 citation statements)

References 28 publications

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“…Ubiquitin conjugating enzyme E2 L6 (UBE2L6) serves as an E2 enzyme for post-translational addition of an ubiquitin-like protein ISG15 which is vital for antiviral immunity ( Skaug and Chen, 2010 ) and is involved in the type-I interferon response in active TB disease ( Ottenhoff et al, 2012 ). Vesicle-associated membrane protein 5 (VAMP5) is a member of the SNARE protein family, which regulates the docking and fusion of intracellular membrane vesicles ( Hong, 2005 ) and is involved in the development or function of the respiratory system ( Ikezawa et al, 2018 ). VAMP5 controls intracellular transport events, including endocytosis, exocytosis, and internal recycling ( Tajika et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

The Evaluation and Validation of Blood-Derived Novel Biomarkers for Precise and Rapid Diagnosis of Tuberculosis in Areas With High-TB Burden

Gong

Xiong

et al. 2021

Front. Microbiol.

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Tuberculosis (TB) remains a highly contagious public health threat. Precise and prompt diagnosis and monitoring of treatment responses are urgently needed for clinics. To pursue novel and satisfied host blood-derived biomarkers, we streamlined a bioinformatic pipeline by integrating differentially expressed genes, a gene co-expression network, and short time-series analysis to mine the published transcriptomes derived from whole blood of TB patients in the GEO database, followed by validating the diagnostic performance of biomarkers in both independent datasets and blood samples of Chinese patients using quantitative real-time PCR (qRT-PCR). We found that four genes, namely UBE2L6 (Ubiquitin/ISG15-conjugating enzyme E2 L6), BATF2 (Basic leucine zipper transcriptional factor ATF-like), SERPING1 (Plasma protease C1 inhibitor), and VAMP5 (Vesicle-associated membrane protein 5), had high diagnostic value for active TB. The transcription levels of these four gene combinations can reach up to 88% sensitivity and 78% specificity (average) for the diagnosis of active TB; the highest sensitivity can achieve 100% by parallel of BATF2 and VAMP5, and the highest specificity can reach 89.5% through a combination of SERPIG1, UBE2L6, and VAMP5, which were significantly higher than 75.3% sensitivity and 69.1% specificity by T-SPOT.TB in the same patients. Quite unexpectedly, the gene set can assess the efficacy of anti-TB response and differentiate active TB from Latent TB infection. The data demonstrated these four biomarkers might have great potency and advantage over IGRAs in the diagnosis of TB.

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Section: Discussionmentioning

confidence: 99%

The Evaluation and Validation of Blood-Derived Novel Biomarkers for Precise and Rapid Diagnosis of Tuberculosis in Areas With High-TB Burden

Gong

Xiong

et al. 2021

Front. Microbiol.

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“…1d). Validity of the VAMP5 antibody (Supplementary Table 1) was indicated by the observation that VAMP5-deficient mice show strongly reduced signals in immunoblots and immunohistochemical staining performed with the same antibody (Ikezawa et al, 2018). Moreover, VAMP5 appeared as a top enriched protein in immunoprecipitated retinal lysates analysed by mass spectrometry (Supporting Data 1).…”

Section: Resultsmentioning

confidence: 99%

“…VAMP5 has also been shown to be part of the machinery that mediates EV-dependent cytokine release from 26 mesenchymal stem cells in vitro (Kou et al, 2018). VAMP5 did not mediate fusion of vesicular and plasma membrane containing specific tSNAREs in an artificial exocytosis assay (Hasan et al, 2010), but deletion of VAMP5 from mice causes developmental defects in the respiratory and urinary systems and perinatal death (Ikezawa et al, 2018). The observed upregulation of VAMP5 and the changes in CD9 distribution under ischemic conditions suggest that VAMP5 is part of the gliotic reaction to injury and disease and that ischemia affects EV secretion in the retina.…”

Section: Discussionmentioning

confidence: 99%

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Release of VAMP5-positive extracellular vesicles by retinal Müller gliain vivo

Demais

Pohl

Wunderlich

et al. 2022

Preprint

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Cell-cell interactions in the central nervous system (CNS) are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles (EVs). The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Müller cells, a type of glial cells in the retina, which forms part of the CNS. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct EVs from highly specialized domains of Müller cells in retinae from adult mice in vivo. We identify VAMP5 as a Müller cell-specific SNARE component that is part of EVs and responsive to ischemia, and we reveal differences between the secretomes of affinity-purified Müller cells and neurons in vitro. Our findings suggest EV-based communication as an important mediator of cellular interactions in the retina.

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“…This correlation can add molecular data to the 3D morphological data, although the molecular data are limited to 2D distribution. To date, the CoMBI-C system has been used in various research fields, such as 3D analysis of primordia of beetle horn 16 , 3D distribution analysis of myelinated fibers of human facial nerves 17 , 3D morphological analysis of polycystic kidney of knockout mice 18 , and 3D visualization of regenerating bones and cartilage of the axolotl jaw 19 . Through these collaborative studies and ongoing collaborations, we found that users of the conventional CoMBI-C system desired compatibility with not only frozen specimens but also paraffin-embedded specimens.…”

Section: Introductionmentioning

confidence: 99%

Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens

Ishii

Tajika

Murakami

et al. 2021

Sci Rep

Self Cite

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Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the morphological analyses of various biological specimens, and its use is expanding. However, the conventional CoMBI system utilizes a cryostat, which limits its compatibility to only frozen blocks and the resolution of the block-face image. We developed a new CoMBI system that can be applied to not only frozen blocks but also paraffin blocks, and it has an improved magnification for block-face imaging. The new system, called CoMBI-S, comprises sliding-type sectioning devices and imaging devices, and it conducts block slicing and block-face imaging automatically. Sections can also be collected and processed for microscopy as required. We also developed sample preparation methods for improving the qualities of the block-face images and 3D rendered volumes. We successfully obtained correlative 3D datasets and 2D microscopic images of zebrafish, mice, and fruit flies, which were paraffin-embedded or frozen. In addition, the 3D datasets at the highest magnification could depict a single neuron and bile canaliculus.

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Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung

Cited by 12 publications

References 28 publications

The Evaluation and Validation of Blood-Derived Novel Biomarkers for Precise and Rapid Diagnosis of Tuberculosis in Areas With High-TB Burden

The Evaluation and Validation of Blood-Derived Novel Biomarkers for Precise and Rapid Diagnosis of Tuberculosis in Areas With High-TB Burden

Release of VAMP5-positive extracellular vesicles by retinal Müller gliain vivo

Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens

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