Loss of viability in rye embryos at different levels of hydration: senescence with apoptotic nucleosome cleavage or death with random DNA fragmentation.

Boubriak, Ivan; Naumenko, V.; Lyne, Linden; Osborne, Daphne J.; Black, Max; Bradford, Kent J.; Vázquez‐Ramos, Jorge M.

doi:10.1079/9780851994048.0205

Cited by 22 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even the physiological and biochemical changes have shown variations in the patterns of deterioration among these two age groups both in soybean and safflower (Vijay and Dadlani 2003). Boubriak et al (1999) observed that rye embryos held at 9% moisture content show a progressive loss of viability during 4 years of storage, whereas those exposed to accelerated aging conditions (at a moisture content of about 14%) showed an accumulation of DNA nucleosome multimers rather than random fragmentation. They concluded that specific nucleases are activated at different levels of hydration.…”

Section: Discussionmentioning

confidence: 94%

“…In addition, one of the first events to occur in a viable embryo upon hydration is an active repair of such breaks and lesions, which restores genomic integrity (Boubriak et al 1999). Schoen et al (1998) and Chwedorzewska et al (2002a, b) have also suggested that mutation accumulations occur in long-term storage of germplasm and that such mutations have the potential to reduce the viability as well as alter the genetic makeup of the conserved material.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

et al. 2009

View full text Add to dashboard Cite

Storage of seeds for extended periods causes a number of degradative changes related to the aging process such as decreased seedling vigor and reduced germination. In this study, molecular markers were used to study the aging process in seeds of two different plants species. Seeds of three differentially aged seed groups, including control (un-aged), naturally aged, and accelerated aging, from soybean (Glycine max) and safflower (Carthamus tinctorius) were evaluated for genetic variability using random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. For both plant species, naturally aged and accelerated aged groups clustered together with RAPD markers, whereas control and naturally aged seeds showed similarity in both AFLP and SSR profiles. Based on these findings, it can be concluded that observed changes in DNA profiles of seeds from different aged groups did not contribute to accumulation of genetic variations of the same magnitude. Therefore, seed of similar viability must be selected for molecular marker analysis for plant variety protection, among other comparative studies.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

et al. 2009

View full text Add to dashboard Cite

show abstract

“…In a study of rye seeds, DNA was progressively cleaved as seeds dried, deteriorated and lost their ability to germinate (Cheah & Osborne 1978; Osborne, Sharon & Ben‐Ishai 1981). DNases operating at different levels of water activity were the cause of this DNA cleavage (Boubriak et al . 2000).…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration

Wang

et al. 2012

Plant Cell & Environment

View full text Add to dashboard Cite

Seed deterioration is poorly understood and remains an active area for research. Seeds of elm (Ulmus pumila L.) were aged at 37°C above water [controlled deterioration treatment (CDT)] for various lengths of time to assess programmed cell death (PCD) and reactive oxygen species (ROS) product in embryonic tissues during a 5 d period. The hallmarks of PCD were identified in the elm seeds during CDT including TUNEL experiments, DNA laddering, cytochrome c (cyt c) leakage and enzymatic activities. These analyses indicated that PCD occurred systematically and progressively in deteriorated elm seeds. Cyt c release and increase in caspase-3-like/DEVDase activity occurred during CDT, which could be suppressed by ascorbic acid (AsA) and caspase-3 inhibitor Ac-DEVD-CHO, respectively. In situ localization of ROS production indicated that the distinct spatial-temporal signature of ROS during CDT coincided with the changes in PCD hallmark features. Multiple antioxidant elements were activated during the first few days of CDT, but were subsequently depleted as PCD progressed. Taken together, our findings identify PCD as a key mechanism that occurs asymmetrically during elm seeds CDT and suggest an important role for PCD in seeds deterioration.

show abstract

“…Some studies have suggested that seed deterioration may belong to PCD. During seed deterioration, DNA fragmentation was cut off at the linking region of the nucleosome (Boubriak et al, 2000). Nucleo-cytoplasimic cleavage of lichee and longan were observed during the aging of seeds (Peng and Fu, 1996).…”

Section: Discussionmentioning

confidence: 97%

“…Nuclear and cytoplasm separation and DNA fragmentation were found in seed cells of lichee and longan during deterioration (Peng and Fu, 1996). During seed deterioration, DNA was cut from nucleosomes (Boubriak et al, 2000). These phenomena are similar to some characteristics of PCD, although systematic research into PCD during seed aging has not been reported until now.…”

Section: Introductionmentioning

confidence: 97%

Programmed cell death of Ulmus pumila L. seeds during aging

Zhang

et al. 2008

Front. For. China

View full text Add to dashboard Cite

The programmed cell death (PCD) characteristics of Ulmus pumila L. seeds were investigated. The seeds were treated at a high temperature of 37uC and 100% relative humidity for six days. DAPI (4'6-diamidino-2-phenylindole) staining revealed that the aging treatment induced condensation and margination of chromatin, as well as the formation of apoptotic bodies. DNA electrophoresis results of U. pumila seeds on an agarose gel showed a characteristic ''ladder'' pattern. Levels of electrolyte leakage of seed cells showed that membranes retained their integral form during almost the entire aging time. There was an immediate increase in the production rate of superoxide anion (O 2 2 ) and in the amount of hydrogen peroxide (H 2 O 2 ), which remained at a mmol level. All of these common characteristics indicate that seed aging can be classified as PCD.

show abstract

Loss of viability in rye embryos at different levels of hydration: senescence with apoptotic nucleosome cleavage or death with random DNA fragmentation.

Cited by 22 publications

References 0 publications

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower

Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration

Programmed cell death of Ulmus pumila L. seeds during aging

Contact Info

Product

Resources

About