Louis Pasteur: the first experimental aerobiologist

Ariatti, Annalisa; Comtois, Paul

doi:10.1007/bf02311365

Cited by 18 publications

(13 citation statements)

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“…Investigations on the ambient bacteria experienced a recent growth in interest with the aerosol community. To our knowledge, the first study on airborne microorganisms was reported in the mid-19th century 15 .…”

Section: Introductionmentioning

confidence: 99%

Profile of inhalable bacteria in PM2.5 at Mt. Tai, China: Abundance, community, and influence of air mass trajectories

Wei

Chen

et al. 2019

Ecotoxicology and Environmental Safety

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Highlights 1. PM 2.5 samples exhibited a high microbial biodiversity and complex chemical composition 2. Relationships between bacterial populations and PM composition were investigated. 3. The highest bacterial concentration and more potential pathogens were recorded under the influence of local air pollutants. 4. The long-range transported air mass exhibited highest richness and diversity with an increased abundance of dust-borne bacteria.

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Section: Introductionmentioning

confidence: 99%

Profile of inhalable bacteria in PM2.5 at Mt. Tai, China: Abundance, community, and influence of air mass trajectories

Wei

Chen

et al. 2019

Ecotoxicology and Environmental Safety

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“…The low concentrations of interest lead to detection limits and sensitivity problems in subsequent analysis. The second issue is the fact that bioaerosols are highly dynamic in time and space [ 13 , 20 – 23 ]. This large variability makes it difficult to establish and compare protocols from various studies.…”

Section: Introductionmentioning

confidence: 99%

“…Culture-based and microscopic methods dominated early bioaerosol research [ 2 , 20 , 24 ] and are still used today [ 25 – 28 ] because of their practicality and specificity. However, microscopic methods are generally labour intensive, while the culture based methods might carry a certain level of bias particularly when a broader range of microorganisms are targeted.…”

Section: Introductionmentioning

confidence: 99%

Protocol Improvements for Low Concentration DNA-Based Bioaerosol Sampling and Analysis

Luhung

et al. 2015

PLoS ONE

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IntroductionAs bioaerosol research attracts increasing attention, there is a need for additional efforts that focus on method development to deal with different environmental samples. Bioaerosol environmental samples typically have very low biomass concentrations in the air, which often leaves researchers with limited options in choosing the downstream analysis steps, especially when culture-independent methods are intended.ObjectivesThis study investigates the impacts of three important factors that can influence the performance of culture-independent DNA-based analysis in dealing with bioaerosol environmental samples engaged in this study. The factors are: 1) enhanced high temperature sonication during DNA extraction; 2) effect of sampling duration on DNA recoverability; and 3) an alternative method for concentrating composite samples. In this study, DNA extracted from samples was analysed using the Qubit fluorometer (for direct total DNA measurement) and quantitative polymerase chain reaction (qPCR).Results and FindingsThe findings suggest that additional lysis from high temperature sonication is crucial: DNA yields from both high and low biomass samples increased up to 600% when the protocol included 30-min sonication at 65°C. Long air sampling duration on a filter media was shown to have a negative impact on DNA recoverability with up to 98% of DNA lost over a 20-h sampling period. Pooling DNA from separate samples during extraction was proven to be feasible with margins of error below 30%.

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“…However, the erroneous “miasma” theory of pathogen transmission continued to dominate, and Fracastoro's “seed of disease” contagion theory was not accepted until the development of germ theory by Louis Pasteur (1822–1895) and Robert Koch (1843–1910). Pasteur also discovered that microbes were present in air 8 …”

Section: Figurementioning

confidence: 99%

Basic routes of transmission of respiratory pathogens—A new proposal for transmission categorization based on respiratory spray, inhalation, and touch

2021

Indoor Air

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Louis Pasteur: the first experimental aerobiologist

Cited by 18 publications

References 0 publications

Profile of inhalable bacteria in PM2.5 at Mt. Tai, China: Abundance, community, and influence of air mass trajectories

Profile of inhalable bacteria in PM2.5 at Mt. Tai, China: Abundance, community, and influence of air mass trajectories

Protocol Improvements for Low Concentration DNA-Based Bioaerosol Sampling and Analysis

Basic routes of transmission of respiratory pathogens—A new proposal for transmission categorization based on respiratory spray, inhalation, and touch

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